Evaluation of <i>Trichoderma harzianum</i> for controlling root rot caused by <i>Phytophthora capsici</i> in pepper plants

Ahmed, Aneesh; Pérez-Sánchez, Cristina; Egea, Catalina; Castillo, María Emilia Candela

doi:10.1046/j.1365-3059.1999.00317.x

Cited by 65 publications

(34 citation statements)

References 21 publications

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“…Several species of Trichoderma have been shown to be particularly effective in the control of P. capsici, and are used as biocontrol agents for controlling pepper blight (Sid Ahmed et al, 1999;Ezziyyani et al, 2007;Osorio-Hernandez et al, 2011). However, it is unclear whether these fungi also have antagonistic activities against the oospores of P. capsici.…”

Section: Discussionmentioning

confidence: 99%

“…against P. capsici, has also been examined and the antagonistic isolates were screened. The Trichoderma species, such as T. harzianum, have excellent biocontrol potential (Sid Ahmed et al, 1999;Ezziyyani et al, 2007;Osorio-Hernandez et al, 2011). Despite ongoing trials to identify the significant biocontrol effects of using the Trichoderma species on hyphae of P. capsici, no study has proven that the Trichoderma species are able to infect the oospores of P. capsici.…”

Section: Introductionmentioning

confidence: 99%

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Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

Jiang

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Phytophthora capsici is a phytopathogen that causes a destructive pepper blight that is extremely difficult to control. Using a fungicide application against the disease is costly and relatively ineffective and there is also a huge environmental concern about the use of such chemicals. The genus Trichoderma has been known to have a potential biocontrol issue. In this paper we investigate the mechanism for causing the infection of T. asperellum against P. capsici. Trichoderma sp. (isolate CGMCC 6422) was developed to have a strong antagonistic action against hyphae of P. capsici through screening tests. The strain was identified as T. asperellum through using a combination of morphological characteristics and molecular data. T. asperellum was able to collapse the mycelium of the colonies of the pathogen through dual culture tests by breaking down the pathogenic hyphae into fragments. The scanning electron microscope showed that the hyphae of T. asperellum surrounded and penetrated the pathogens hyphae, resulting in hyphal collapse. The results show that seven days after inoculation, the hyphae of the pathogen were completely degraded in a dual culture. T. asperellum was also able to enter the P. capsici oospores through using oogonia and then developed hyphae and produced conidia, leading to the disintegration of the oogonia and oospores. Seven days after inoculation, an average 10.8% of the oospores were infected, but at this stage, the structures of oospores were still intact. Subsequently, the number of infected oospores increased and the oospores started to collapse. Forty-two days after inoculation, almost all the oospores were infected, with 9.3% of the structures of the oospores being intact and 90.7% of the oospores having collapsed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

Jiang

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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“…[26], F.oxysporum f.sp. lycopersici [27], Rhizoctonia solani [28], Scerotium rolfsii [29], Phytopthora capsici [17], Gaeumannomyces graminis var. tritici [30].…”

Section: Resultsmentioning

confidence: 99%

“…Plates were incubated for 2 days at 15 o C and one 5-mm diameter agar disc of F.oxysporum from a 1-week-old PDA culture was placed in the centre of the plate. After 7 days of incubation at 28±2 o C, the width of the inhibition zone was measured for each P.fluorescens isolate and the inhibition was calculated according to the formula given by [17].…”

Section: Collection and Screening Of Soil Samples For Biocontrol Agentsmentioning

confidence: 99%

BIOLOGICAL CONTROL OF F. OXYSPORUM f. sp. LYCOPERSICI CAUSING WILT OF TOMATO BY PSEUDOMONAS FLUORESCENS

BB¹,

S²,

AC³

et al. 2011

Int J of Micr Res

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Abstract-Pseudomonas fluorescens is one of the major fungal biocontrol agents found in the soil and the rhizosphere of various crop systems. Ten isolates of P.fluorescens were isolated from rhizosphere soil samples collected from various tomato-growing fields and evaluated for their efficacy in increasing seed quality variables of tomato and in inhibiting the mycelial growth of Fusarium oxysporum. Pseudomonas isolate 2 produced effective results and was selected and mass multiplied. Talc and sodium alginate formulations of mass multiplied using different agents were prepared and evaluated for their effects against fusarium wilt under greenhouse conditions. Fresh cultures of Pf2 isolate was found to increase seedling emergence and reduce fusarium wilt disease incidence when compared to the control and the formulations.

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“…suppress phytopathogens are basically three, i.e. direct competition for space or nutrients [28][29][30], the production of antibiotic metabolites, whether volatile or not [31,32] and direct parasitism on phytopathogenic fungi [33]. Furthermore, the genus Trichoderma possesses good qualities for controlling diseases in plants caused by soil borne pathogens, especially those of the genera Phytophthora, Rhizoctonia [34,35], Pythium [36,37], Fusarium [34,38,39] and Macrophomina [34].…”

Section: Discussionmentioning

confidence: 99%

Rhizoctonia Root Rot of Pepper (Capsicum annuum): Comparative Pathogenicity of Causal Agent and Biocontrol Attempt using Fungal and Bacterial Agents

Mannai¹,

Jabnoun‐Khiareddine²,

Nasraoui³

et al. 2018

J Plant Pathol Microbiol

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Rhizoctonia root rot of pepper (Capsicum annuum L.) is becoming serious in Tunisia. Comparative pathogenicity tests performed for Rhizoctonia solani isolates recovered from pepper and potato showed that Rhiz.7 and Rhiz.4 were the most aggressive. They reduced by 53.5%-91.4% the aerial part fresh weight of inoculated cv. Baklouti plants relative to control. Rhiz.7 decreased by 81%-88% the root fresh weight on cvs. Beldi and Baklouti. Various fungal and bacterial agents were tested against R. solani. Dual culture trials showed that Trichoderma harzianum, T. viride and Glicladium virens grew and sporulated profusely over R. solani colonies and altered its hyphae. Pseudomonas huttiensis 69, P. aureofaciens 31 and Burkholderia glathei 35 reduced pathogen growth by 9.71-12.87%. These bio-agents were tested for their effects on rhizoctonia root rot disease and pepper growth. On cv. Beldi, pre-emergence damping-off, noted after 15 days, was suppressed by 55 (for G. virens), 45 (for T. viride) and 50% (for T. harzianum). This inhibition reached 57.14% using Bacillus pumilus 420 and P. putida 227. Tested on pepper cv. Altar, all tested fungi decreased by 40% post-emergence damping-off, and significantly increased the plant height of R. solani-inoculated and treated plants by 21.13 (for T. viride) to 36.34% (for T. harzianum) relative to control. P. aureofaciens 314 and P. putida 227 completely suppressed R. solani post-emergence expression. Treatments with P. aureofaciens 314, P. aureofaciens 31, Bacillus pumilus 420, P. fluorescens Pf and P. putida 227 induced a significant increase in their height compared to control. An improvement of the aerial part fresh weight by 54.54, 48.09 and 47.74%, as compared to control, was induced by P. aureofaciens 314, B. glathei 35 and P. huttiensis 69, respectively. Indeed, strains of B. thuringensis were found to be efficient for the biocontrol of R. solani of chili pepper based on in vitro assays [20]. Moreover, B. cepacia was shown able to reduce the severity of Rhizoctonia diseases associated to pepper and tomato [19]. Antibiosis seems to be their principal mode of action [21].Pseudomonas species were shown capable of markedly inhibiting the growth of R. solani in vitro and in vivo. Indeed, tomato plants were also highly protected against R. solani infestations using this bacterium suspended in water [19]. Moreover, fluorescent Pseudomonas species were found to induce systemic resistance in plants as a result of root colonization [18].Recently, several rhizobacterial isolates and mainly B. thuringiensis B2 (KU158884), B. subtilis B10 (KT921327) and Enterobacter cloacae B16 (KT921429) were found to be efficient for the suppression of R. solani radial growth and disease severity and for the enhancement of tomato growth [10].In Tunisia, R. solani is still being a destructive pathogen of pepper and investigations for its biocontrol are lacking. Therefore, the objectives of the current study were: (i) to evaluate the aggressiveness of different R. solani isolates involved in dam...

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Evaluation of Trichoderma harzianum for controlling root rot caused by Phytophthora capsici in pepper plants

Cited by 65 publications

References 21 publications

Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

BIOLOGICAL CONTROL OF F. OXYSPORUM f. sp. LYCOPERSICI CAUSING WILT OF TOMATO BY PSEUDOMONAS FLUORESCENS

Rhizoctonia Root Rot of Pepper (Capsicum annuum): Comparative Pathogenicity of Causal Agent and Biocontrol Attempt using Fungal and Bacterial Agents

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