Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Iglesias, Juan Manuel Pérez; Arcaute, Celeste Ruiz de; Natale, Guillermo Sebastián; Soloneski, Sonia; Larramendy, Marcelo L.

doi:10.1016/j.ecoenv.2017.04.054

Cited by 32 publications

(12 citation statements)

References 34 publications

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“…For instance, it has been found to cause oxidative stress and endocrine disruption in rats (Yang et al 2018) and developmental toxicity associated to thyroid dysfunction in zebrafish (Li et al 2020). Finally, imazethapyr has also been reported by causing metabolic alterations in Cyprinus carpio (Moraes et al 2011), genotoxicity in tadpoles of Rhinella arenarum and Hypsiboas pulchellus species (Carvalho et al 2019;Perez-Iglesias et al 2017;Perez-Iglesias et al 2015), and also in mammalian cells (Soloneski et al 2017); however, the toxicity of herbicide is poorly characterized and needs to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Kuhn

Jacques

Teixeira

et al. 2021

Environ Sci Pollut Res

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Uruguay River is the most important river in western Rio Grande do Sul, separating Brazil from Argentina and Uruguay. However, its pollution is of great concern due to agricultural activities in the region and the extensive use of pesticides. In a long term, this practice leads to environmental pollution, especially to the aquatic system. The objective of this study was to analyze the physicochemical characteristics, metals and pesticides levels in water samples obtained before and after the planting and pesticides' application season from three sites: Uruguay River and two minor affluents, Mezomo Dam and Salso Stream. For biomonitoring, the free-living nematode Caenorhabditis elegans was used, which were exposed for 24 h. We did not find any significant alteration in physicochemical parameters. In the pre-and post-pesticides' samples we observed a residual presence of three pesticides (tebuconazole, imazethapyr, and clomazone) and metals which levels were above the recommended (As, Hg, Fe, and Mn). Exposure to both pre-and post-pesticides' samples impaired C. elegans reproduction and post-pesticides samples reduced worms' survival rate and lifespan. PCA analysis indicated that the presence of metals and pesticides are important variables that impacted C. elegans biological endpoints. Our data demonstrates that Uruguay River and two affluents are contaminated independent whether before or after pesticides' application season. In addition, it reinforces the usefulness of biological indicators, since simple physicochemical analyses are not sufficient to attest water quality and ecological safety.

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Section: Discussionmentioning

confidence: 99%

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Kuhn

Jacques

Teixeira

et al. 2021

Environ Sci Pollut Res

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“…The conducted studies mainly focused on the evaluation of environmental stressors such as agrochemicals and heavy metals in vivo. [223,[227][228][229][230][231][232][233][234][235][236][237], fungicides (captan) [238], pesticides [239,240], antiparasitic agents [239], persistent organic pollutants (POPs) [219] as well as other agrochemicals [241] and poultry litter [242]. These studies indicated the DNA damaging effects of selected chemicals as well as using amphibians as sentinel organisms for the evaluation of environmental pollution.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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“…The SCGE assay is a sensitive, rapid, and reliable method to determine DNA damage and can be considered as a nonspecific biomarker for genotoxicity of aquatic organisms (Vera‐Candioti et al 2013; Pérez‐Iglesias et al 2017). In our study, SCGE analysis revealed obvious DNA damage in all the fish samples and indicated that there were significant differences in DNA damage between fish samples from the Helong Reservoir and the controls from the upper reaches of the Liuxihe Reservoir ( p < 0.05); L. rohita was also the species most sensitive to DNA damage among the collected fish.…”

Section: Discussionmentioning

confidence: 99%

Indigenous Fish–Based Assessment of Genotoxic Potentials of the Helong Reservoir in Guangzhou, China

Xian

Tang

Chen

et al. 2021

Environmental Toxicology and Chemistry

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The present study was conducted to assess the genotoxic potential of water from the Helong Reservoir, which was designated as a strategic drinking water source by the Guangdong Provincial Government of China in October 2016. Four kinds of common indigenous fish samples (Labeo rohita, Cirrhinus molitorella, red tilapia, and Oreochromis niloticus) were collected at 6 sampling sites during the period from July to November 2020. Fish from the clean drinking water source of the upper reaches of the Liuxihe Reservoir in Guangzhou were collected as the control. Both the alkaline single cell gel electrophoresis assay and the micronucleus test were used to detect DNA damage and the micronucleus rate in erythrocytes of fish samples, respectively. The results indicated that there was a significant increase in comet tail length, Olive tail moment, and micronucleus rates of all fish samples compared with those of the control (p < 0.05). The order of sensitivity to DNA damage and micronucleus formation was Labeo rohita > Cirrhinus molitorella > red tilapia > Oreochromis niloticus. The results of the 2 kinds of experiments were in perfect agreement with each other. We conclude that there are obvious genotoxic effects from the water in the Helong Reservoir. As a strategic drinking water source, the safety of the Reservoir water quality should be considered. The local government should put the restoration of the Helong Reservoir water quality on the agenda as soon as possible.

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Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Cited by 32 publications

References 34 publications

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

Ecotoxicological assessment of Uruguay River and affluents pre- and post-pesticides’ application using Caenorhabditis elegans for biomonitoring

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Indigenous Fish–Based Assessment of Genotoxic Potentials of the Helong Reservoir in Guangzhou, China

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