Evaluation of immune efficacy of Omp2 protein against Lawsonia intracellularis in mice

Li, Minxue; Xiao, Ni; Li, Jiannan; Lin, Huixing; Fan, Hongjie

doi:10.1016/j.vetmic.2021.109274

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Moreover, the enormous progress in the computational design of epitope-based vaccines could improve vaccine efficacy by selecting the accurate antigen composition, linkers and tags, and B and T epitopes, and such an improvement, in turn, facilitates antigen processing, increases the specificity of the vaccine candidate, and supports a long-lasting immune response [ 22 , 23 ]. A great breakthrough in the research of a recombinant vaccine against L. intracellularis was the sequencing of the bacterial genome [ 24 , 25 ], encouraging researchers to identify potential antigens against the pathogen to design and develop recombinant vaccine candidates [ 26 , 27 , 28 ]. In a previous study, our group produced a recombinant vaccine candidate to prevent PPE based on three antigenic proteins from L. intracellularis [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

New Formulation of a Subunit Vaccine Candidate against Lawsonia intracellularis Increases Humoral and Cellular Immune Responses

Salazar,

Starck,

Villegas

et al. 2023

Vaccines

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Previously, we designed a subunit vaccine candidate based on three L. intracellularis antigens with promising results in pigs. In this study, antigens were produced individually to achieve an even antigen ratio in the formulation. The emulsion characterization included the drop size and the mechanical and thermal stability. Immune response was evaluated by indirect and sandwich ELISAs, qPCR, and flow cytometry. The vaccine candidate’s safety was assessed by histopathology and monitoring the clinical behavior of animals. The average production yielded for the chimeric antigen as inclusion bodies was around 75 mg/L. The formulation showed mechanical and thermal stability, with a ratio Hu/Ho > 0.85 and a drop size under 0.15 nm. Antigens formulated at a ratio of 1:1:1 induced a significant immune response in inoculated pigs that persisted until the end of the experiment (week 14). The dose of 200 μg significantly activated cellular response measured by transcriptional and translational levels of cytokines. The cell proliferation assay revealed an increment of lymphocytes T CD4+ at the same dose. Animals gained weight constantly and showed proper clinical behavior during immunization assays. This research demonstrated the immunological robustness of the new subunit vaccine candidate against Porcine Proliferative Enteropathy evenly formulated with three chimeric antigens of L. intracellularis.

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