Evaluation of immune responses in HIV infected patients with pleural tuberculosis by the QuantiFERON®TB-Gold interferon-gamma assay

Baba, Kamaldeen; Sørnes, Steinar; Hoosen, Anwar A.; Lekabe, Jacob M.; Mpe, Mathew J.; Langeland, Nina; Dyrhol‐Riise, Anne Ma

doi:10.1186/1471-2334-8-35

Cited by 52 publications

(59 citation statements)

References 31 publications

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“…NPV in patients with active tuberculosis 18 articles satisfied our inclusion criteria for evaluating the NPV; 13 assessed the NPV among confirmed tuberculosis cases [22,26,[34][35][36][37][38][39][40][41][42][43][44] and six assessed the prospective outcome in IGRA-negative individuals after an average of 2 yrs [19-22, 45, 46]. Among the studies in which the NPV was evaluated in patients with confirmed tuberculosis (387 tuberculosis cases with a valid T-SPOT1.TB result and 304 with valid QFT-G-IT result), the NPV varied greatly, irrespective of the IGRA used.…”

Section: Specificitymentioning

confidence: 99%

Interferon-γ release assays for the diagnosis of latentMycobacterium tuberculosisinfection: a systematic review and meta-analysis

Diel¹,

Goletti²,

Ferrara³

et al. 2010

Eur Respir J

492

315

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We conducted a systematic review and meta-analysis to compare the accuracy of the QuantiFERON-TB1 Gold In-Tube (QFT-G-IT) and the T-SPOT1.TB assays with the tuberculin skin test (TST) for the diagnosis of latent Mycobacterium tuberculosis infection (LTBI).The Medline, Embase and Cochrane databases were explored for relevant articles in November 2009. Specificities, and negative (NPV) and positive (PPV) predictive values of interferon-c release assays (IGRAs) and the TST, and the exposure gradient influences on test results among bacille Calmette-Guérin (BCG) vaccinees were evaluated.Specificity of IGRAs varied 98-100%. In immunocompetent adults, NPV for progression to tuberculosis within 2 yrs were 97.8% for T-SPOT1.TB and 99.8% for QFT-G-IT. When test performance of an immunodiagnostic test was not restricted to prior positivity of another test, progression rates to tuberculosis among IGRA-positive individuals followed for 19-24 months varied 8-15%, exceeding those reported for the TST (2-3%). In multivariate analyses, the odd ratios for TST positivity following BCG vaccination varied 3-25, whereas IGRA results remained uninfluenced and IGRA positivity was clearly associated with exposure to contagious tuberculosis cases.IGRAs may have a relative advantage over the TST in detecting LTBI and allow the exclusion of M. tuberculosis infection with higher reliability.

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Section: Specificitymentioning

confidence: 99%

Interferon-γ release assays for the diagnosis of latentMycobacterium tuberculosisinfection: a systematic review and meta-analysis

Diel¹,

Goletti²,

Ferrara³

et al. 2010

Eur Respir J

492

315

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show abstract

“…In the last several months, two case-control studies from high-burden countries (South Africa) and using QFT-TB-GIT have been published [15,16]. Both showed that the QFT-TB-GIT assay had suboptimal sensitivity (27 and 57%, respectively).…”

Section: Pleural Hbha and Ppd Elispot Responsesmentioning

confidence: 99%

“…We therefore hypothesised that TB antigen-specific responses could distinguish active pleural TB from alternative diagnoses and should have better discriminatory value than unstimulated IFN-c, a nonspecific biomarker of inflammation. A recent case-control study from Europe showed a high diagnostic accuracy with the T-SPOT.1TB assay (Oxford Immunotec, Abingdon, UK) [13] but overall data on the utility of two known IFN-c release assays (IGRAs), T-SPOT.1TB and QuantiFERON1-TB Gold In-tube (QFT-TB-GIT; Cellestis, Carnegie, Australia), including a head-to-head comparison, for the diagnosis of TB-PE are limited [13][14][15][16]. There are several other unresolved questions, including the optimal cell number and pleural fluid volume required, optimal cut-off point and utility in high HIV-prevalence settings [17].…”

mentioning

confidence: 99%

Utility of quantitative T-cell responsesversusunstimulated interferon-γ for the diagnosis of pleural tuberculosis

Dheda

Zyl-Smit²,

Sechi³

et al. 2009

Eur Respir J

110

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The clinical utility of antigen-specific interferon (IFN)-c release assays (IGRAs) using pleural mononuclear cells, for the diagnosis of tuberculosis (TB), requires clarification.We compared the diagnostic utility of unstimulated pleural IFN-c levels with several pleural antigen-specific T-cell IGRAs (early secretory antigenic target-6 and culture filtrate protein-10 (T-SPOT.1TB, QuantiFERON1-TB Gold In-tube), purified protein derivative (PPD) and heparinbinding haemagglutinin (HBHA)) in 78 South African TB suspects. Test results were compared against a clinical score and a reference standard.Out of 74 evaluable subjects 48, seven and 19 had definite, probable and no TB, respectively. 11 (15%) out of 74 pleural samples (nine (19%) out of 48 of the definite TB cases) had total cell counts that were inadequate for T-cell processing. In the remaining 63 samples, the sensitivity, specificity, positive predictive value and negative predictive value of different diagnostic methods were as follows. Maximal bioclinical score: 54, 89, 92 and 43%, respectively; T-SPOT.1TB: 86, 60, 84 and 64%, respectively; QuantiFERON1-TB Gold In-tube: 57, 80, 87 and 44%, respectively; HBHA-specific IGRA: 59, 31, 64 and 27%, respectively; PPD-specific IGRA: 81, 40, 76 and 46%, respectively; and pleural fluid unstimulated IFN-c: 97, 100, 100 and 94%, respectively. Unstimulated IFN-c was the most accurate test for distinguishing TB from non-TB effusions in a high-burden setting. The antigen-specific T-cell IGRAs were limited by suboptimal accuracy and the inability to isolate sufficient mononuclear cells to perform the assay.

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“…In another study of 28 patients with pleural TB, results in pleural fluid were inconclusive in 52% of cases, due to high background IFN-γ production. 79 Commercial IFN-γ tests, T-SPOT.TB and QFT-IT in pleural fluid were compared to unstimulated IFN-γ for the diagnosis of pleural TB in 74 patients. 80 In 11 (15%) cases, the cell counts were not large enough to perform the tests.…”

Section: Pleural Tuberculosismentioning

confidence: 99%

Diagnosis of tuberculosis infection using interferon-γ-based assays

Cerezales

Benítez

2011

Enfermedades Infecciosas y Microbiología Clínica

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Evaluation of immune responses in HIV infected patients with pleural tuberculosis by the QuantiFERON®TB-Gold interferon-gamma assay

Cited by 52 publications

References 31 publications

Interferon-γ release assays for the diagnosis of latentMycobacterium tuberculosisinfection: a systematic review and meta-analysis

Interferon-γ release assays for the diagnosis of latentMycobacterium tuberculosisinfection: a systematic review and meta-analysis

Utility of quantitative T-cell responsesversusunstimulated interferon-γ for the diagnosis of pleural tuberculosis

Diagnosis of tuberculosis infection using interferon-γ-based assays

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