Evaluation of immunoassays for detection of antibodies to human herpesvirus 7

Black, Jodi B.; Schwarz, Tino F.; Patton, Joanne L.; Kite-Powell, Kathleen; Pellett, Philip E.; Wiersbitzky, S; Bruns, R; Müller, Cornelia; Jäger, Gundula; Stewart, John A.

doi:10.1128/cdli.3.1.79-83.1996

Cited by 59 publications

(15 citation statements)

References 23 publications

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“…Immunofluorescent assay was used to evaluate HHV-6 antibody response in this study. However, HHV-6 and human herpesvirus-7 antibodies can cross-react Tanaka et al, 1994;Black et al, 1996], which may have affected the results. It will be necessary to use a more specific serological assay [Yamamoto et al, 1990] in future studies.…”

Section: Discussionmentioning

confidence: 99%

Human herpesvirus 6 infection after living related liver transplantation

et al. 2000

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The aim of the study was to investigate human herpesvirus-6 (HHV-6) infection after liver transplantation from living related donors, and to evaluate the reliability of the presence of HHV-6 DNA in plasma by the polymerase chain reaction (PCR) for monitoring active HHV-6 infection. EDTA peripheral blood was collected from 47 donor and recipient (16 males and 31 females, age 1-320 months) pairs at the time of transplantation and biweekly from these recipients after transplantation until 2 months after operation. Isolation of HHV-6 and serological assays were carried out to evaluate active HHV-6 infection in this study. The presence of the viral DNA in plasma was tested by nested PCR. Four clinical events, such as unexplained fever, thrombocytopenia, rejection, and central nervous system (CNS) involvement, were evaluated for clinical features of the virus infection. Risk factors for the virus activity after liver transplantation were also examined. HHV-6 activity was detected in 23 (49%) of the 47 recipients approximately 2-4 weeks after transplantation. All 9 isolates were HHV-6 variant B. The presence of the viral DNA in plasma correlated well with virus isolation and serology (P < 0.01). Only unexplained fever was associated statistically with HHV-6 activity after liver transplantation (P < 0. 01). If the recipient was seronegative to HHV-6 before transplantation, the recipient was more likely to develop the active virus infection after liver transplantation (P = 0.11). HHV-6 activity occurred in one-half of the recipients approximately 2-4 weeks after liver transplantation, and there was a close association between HHV-6 activity and unexplained fever following transplantation. Detection of the viral DNA in plasma by PCR is useful for monitoring active HHV-6 infection in these patients. Seronegative recipients were more likely to have evidence of active HHV-6 infection after liver transplantation.

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Section: Discussionmentioning

confidence: 99%

Human herpesvirus 6 infection after living related liver transplantation

et al. 2000

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“…Further, a significant association between HHV-6 seroconversion and HCMV disease has been observed in liver transplant recipients [Dockrell et al, 1996]. There is extensive serological cross-reaction between HHV-6 and HHV-7 [Black et al, 1996]. It is therefore possible that the serological response to HHV-6 seen in the liver transplant recipients could reflect either HHV-6 or HHV-7 infection.…”

Section: Discussionmentioning

confidence: 99%

Human herpesvirus-6 and human herpesvirus-7 infections in bone marrow transplant recipients

et al. 1997

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Human cytomegalovirus (HCMV), human herpesvirus-6 (HHV-6), and human herpesvirus-7 (HHV-7) DNA in peripheral blood leukocytes (PBL) of 61 bone marrow transplant recipients was monitored weekly during the first 12 weeks post-transplantation by a nested polymerase chain reaction (PCR). Thirty-seven (61%), 17 (28%), and 32 (53%) of patients had one or more PBL specimens positive for HCMV, HHV-6 or HHV-7 DNA, respectively. HHV-7 DNA in PBL during the early post-transplant period was associated with a longer time to neutrophil engraftment (mean 28.8 days vs 19.8 days; P = 0.01). In two patients who failed to engraft, HHV-6 DNA and HHV-7 DNA was detected in plasma and PBL, respectively, early in their post-transplant period. Patients with HCMV disease were more likely to have concurrent HHV-7 DNA in PBL prior to onset of disease than were patients with asymptomatic HCMV infection, suggesting that HHV-7 may be a cofactor in the progression from HCMV infection to HCMV disease. In the 17 patients (179 specimens) in whom viral DNA in plasma was studied (in addition to PBL), a positive result was found only in 3. In each, viral DNA in plasma appeared to correlate with clinically significant disease. HHV-7 DNA in plasma was associated with encephalitis in an allograft recipient.

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“…Western blot was used to determine serologic status for HHV-6. The method was adapted from one performed by Pellett and colleagues at the Centers for Disease Control [Yamamoto et al, 1990;Black et al, 1996]. Brie¯y, puri®ed HHV-6 antigen (ABi; Columbia, MD) was separated on a sodium dodecyl sulfate-9% polyacrylamide gel by electrophoresis and then transferred to nitrocellulose sheets.…”

Section: Hhv-6 Western Blotmentioning

confidence: 99%

Primary human herpesvirus‐6 associated with an afebrile seizure in a 3‐week‐old infant

Zerr

Yeung

Obrigewitch

et al. 2002

Journal of Medical Virology

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We describe a 3-week-old male infant with an afebrile seizure in whom serologic and polymerase chain reaction (PCR) findings support concomitant primary human herpesvirus 6 (HHV-6) infection. Although HHV-6 infection has been associated with first-time febrile seizures and encephalitis in both immunocompetent and immunocompromised hosts, it has not been associated previously with afebrile seizures in healthy infants. This report provides additional evidence of the neuropathogenic potential of HHV-6.

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Evaluation of immunoassays for detection of antibodies to human herpesvirus 7

Cited by 59 publications

References 23 publications

Human herpesvirus 6 infection after living related liver transplantation

Human herpesvirus 6 infection after living related liver transplantation

Human herpesvirus-6 and human herpesvirus-7 infections in bone marrow transplant recipients

Primary human herpesvirus‐6 associated with an afebrile seizure in a 3‐week‐old infant

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