Evaluation of immunochromatographic test (ICT) strips for the serological detection of Babesia bovis and Babesia bigemina infection in cattle from Western Java, Indonesia

Guswanto, Azirwan; Allamanda, Puttik; Mariamah, Euis Siti; Munkjargal, Tserendorf; Tuvshintulga, Bumduuren; Takemae, Hitoshi; Sivakumar, Thillaiampalam; AbouLaila, Mahmoud; Terkawi, Mohamad Alaa; Ichikawa‐Seki, Madoka; Nishikawa, Yoshifumi; Yokoyama, Naoki; Igarashi, Ikuo

doi:10.1016/j.vetpar.2017.04.010

Cited by 15 publications

(14 citation statements)

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“…We chose recombinant proteins of B. bovis SBP-4 and B. bigemina RAP-1/CT17 as detection antigens in our ELISA and ICT methods. Both proteins showed a high sensitivity and specificity in the detections of specific antibodies against B. bovis and B. bigemina [ 6 , 24 , 25 ]. For nPCR, we chose B. bovis SBP-4 and B. bigemina RAP-1a genes as detection targets.…”

Section: Discussionmentioning

confidence: 99%

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Molecular and serological detection of bovine babesiosis in Indonesia

Guswanto

Allamanda²,

Mariamah³

et al. 2017

Parasites Vectors

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BackgroundBovine babesiosis, mainly caused by Babesia bovis and B. bigemina, is a huge threat to the livestock industry. In Indonesia, the current distribution of the disease is unknown due to a lack of scientific study.MethodsIn the present study, 487 blood samples were collected from cattle with different breeding and age groups in a broad geographical area across the archipelago. The presence of antibodies and current infections of B. bovis and B. bigemina were determined using enzyme-linked immunosorbent assay (ELISA), immunochromatographic test (ICT), and nested PCR (nPCR) targeting B. bovis SBP-4 and B. bigemina RAP-1a genes. Sequence analysis was performed to the amplicon of B. bovis SBP-4, B. bigemina RAP-1a, and internal transcribed spacer (ITS) region of ribosomal RNA of both Babesia species.ResultsIn total, B. bovis positives were detected by ELISA, single-ICT, dual-ICT and nPCR in 340 (69.8%), 317 (65.1%), 307 (63.0%) and 247 (50.7%) samples, respectively. For B. bigemina, the positive samples were detected in 134 (27.5%), 130 (26.7%), 127 (26.1%) and 93 (19.1%), respectively. Furthermore, mixed infections were found in 125 (25.7%), 113 (23.2%), 109 (22.4%) and 52 (10.7%) samples, respectively, which occurred only by chance and were not influenced by additional factors. The obtained nucleotide sequences of B. bovis SBP-4 and B. bigemina RAP-1a genes showed a high homology with other isolates from different countries. Further nucleotide sequence analysis using ITS region showed a great genetic diversity of B. bovis isolates among sampling locations; a lower diversity was found in B. bigemina ITS isolates.ConclusionsThese data revealed the current distribution of B. bovis and B. bigemina infection in cattle in Indonesia. The rate of infection varied among sampling locations, cattle breeds and age groups. Furthermore, B. bovis ITS isolates from Indonesia were found to be more genetically diverse than B. bigemina ITS isolates. The data presented in this study are necessary to develop an effective strategy for controlling the disease in the country.Electronic supplementary materialThe online version of this article (10.1186/s13071-017-2502-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

“…Three types of ICT strips were prepared, bov ICT, big ICT and dual-ICT. The ICTs were based on the recombinant proteins B. bovis SBP-4, B. bigemina RAP-1/CT17, and a combination of both proteins as detection antigens [ 24 , 25 ]. ICT examinations were performed twice on each serum sample.…”

Section: Methodsmentioning

confidence: 99%

Molecular and serological detection of bovine babesiosis in Indonesia

Guswanto

Allamanda²,

Mariamah³

et al. 2017

Parasites Vectors

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“…However, this method requires several steps, including coating, blocking, and results are obtained using an ELISA plate reader. In addition, performing the ELISA experiment requires a laboratory setup and trained personnel, which makes it costly [ 38 , 39 ]. Advances in diagnosis led to the introduction of lateral flow-based immunochromatographic test (ICT) strips, which have gained worldwide popularity because they are cheap, give results in 5–30 min, are applicable in both laboratory and field conditions, and do not require a technical person for interpretation of the outcome [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, performing the ELISA experiment requires a laboratory setup and trained personnel, which makes it costly [ 38 , 39 ]. Advances in diagnosis led to the introduction of lateral flow-based immunochromatographic test (ICT) strips, which have gained worldwide popularity because they are cheap, give results in 5–30 min, are applicable in both laboratory and field conditions, and do not require a technical person for interpretation of the outcome [ 39 , 40 ]. Since their discovery in the 1970s, the strips have been used for various diagnostic purposes including the detection of human chorionic gonadotropin to diagnose pregnancy in humans [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda

et al. 2020

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In Uganda, bovine babesiosis continues to cause losses to the livestock industry because of shortages of cheap, quick, and reliable diagnostic tools to guide prescription measures. In this study, the presence of antibodies to Babesia bigemina and Babesia bovis in 401 bovine blood samples obtained from eastern and central areas of Uganda were detected using enzyme-linked immunosorbent assays (ELISAs) and immunochromatographic test strips (ICTs). The ELISA and ICT test used targeted the B. bigemina C-terminal rhoptry-associated protein (RAP-1/CT17) and B. bovis spherical body protein-4 (SPB-4). Using ELISA, single-ICT and dual-ICT, positive samples for B. bovis were detected in 25 (6.2%), 17 (4.3%), and 14 (3.7%) samples respectively, and positive samples for B. bigemina were detected in 34 (8.4%), 27 (6.7%), and 25 (6.2%), respectively. Additionally, a total of 13 animals (3.2%) had a mixed infection. The correlation between ELISA and single-ICT strips results revealed slight agreement with kappa values ranging from 0.088 to 0.191 between both methods, while the comparison between dual-ICT and single-ICT results showed very good agreement with kappa values >0.80. This study documented the seroprevalence of bovine babesiosis in central and eastern Uganda, and showed that ICT could, after further optimization, be a useful rapid diagnostic test for the diagnosis of bovine babesiosis in field settings.

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“…An evident advantage is that results are provided in less than 15 min. [13] Another study, using an ICT to detect antibodies of B. bovis and B. bigemina both individually and in a dual ICT using serum from bovine samples collected in the field, showed a kappa coefficient > 0.7 when compared to the ELISA [14].…”

Section: Immunochromatographymentioning

confidence: 99%

A Review of Human Babesiosis and the Laboratory Diagnosis

Shen¹,

Liu²

2019

OALib

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Babesiosis is a new worldwide parasitic disease caused by hemoprotozoan parasites of the genus Babesia, which is parasitic on erythrocytes of humans, and other vertebrates, and transmitted via ixodid ticks. Babesiamicroti is the main pathogenic agent of human babesiosis and is prevalent all over the world, including Europe, Asia, Africa, South America, North America and Australia. The United States has the highest number of cases. Babesiosis is mainly distributed in Northeast China, followed by southwest China. As a new insect-borne infectious disease, the disease poses a serious threat to human health, but the research foundation is relatively weak and should be paid more attention to. In this paper, the prevalence, transmission, distribution of babesiosis at home and abroad, and laboratory diagnosis are reviewed. The related research focus of babesiosis mainly includes the screening of diagnostic antigens of babesiosis and the interaction mechanism between Babesia and host and other pathogens.

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Evaluation of immunochromatographic test (ICT) strips for the serological detection of Babesia bovis and Babesia bigemina infection in cattle from Western Java, Indonesia

Cited by 15 publications

References 11 publications

Molecular and serological detection of bovine babesiosis in Indonesia

Molecular and serological detection of bovine babesiosis in Indonesia

Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda

A Review of Human Babesiosis and the Laboratory Diagnosis

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