Evaluation of in-vitro Anti-Inflammatory Activity of Gallic Acid

Abstract

Gallic acid's anti-inflammatory effect was studied at various concentrations, including 50,100,150,200, and 250 ug/ml. Gallic acid's anti-inflammatory effect was assessed using two in-vitro assays: proteinase inhibition and albumin denaturation. The greatest proteinase inhibition activity of 52.83 percent was achieved at a concentration of 250ug/ml, according to the results. It also revealed that at 250ug/ml, the maximum percentage inhibition in albumin denaturation was 74.79 percent. Gallic acid's antiproteinase and albumin denaturation activities both increase with increasing concentrations, according to this research. Keywords: Gallic acid, Anti-Inflammatory, In-Vitro Assays, Albumin Denaturation, Proteinase activity.

The Development and Validation of Analytical Method for Evaluating Gallic Acid in Ethyl Acetate Fraction (Eaf) of Snedds Formulation: Quantitative Analysis With in Vitro Assay

“…Yang et al., 2016 investigated the potential of this compound as a wound healer, with good antioxidant activity, found to stimulate fibroblast cell migration and activate kinase enzymes. Gallic acid can promote wound healing in animal models of both type 1 and type 2 diabetes, particularly in chronic cases [1][2][3].…”

“…Gallic acid can be naturally obtained from plants such as Allan blackia floribunda, Garcinia densivenia, Caesalpinia sappan, Diospyros cinnabarina, Paratecoma peroba, Psidium guajava, Phyllanthus emblica, Terminalia bellirica, Toona sinensis, Oenothera bienni, Rubus suavissimus, Punica granatum, T. bellerica, and Melastoma malabathricum [3][4][5][6][7]. Apridamayanti et al, 2022 conducted fractionation of Melastoma malabathricum leaves and reported gallic acid content of 49.29±0.49 mg/gram when using ethyl acetate as the solvent.…”