Evaluation of in vitro antioxidant activity and HPTLC finger printing analysis of Physalis peruviana fruits

Abstract

In this research work to evaluate in vitro antioxidant activity and HPTLC finger printing analysis of Physalis peruviana fruits. The chemical fingerprinting was carried out by high performance thin layer chromatography. It was carried out by the CAMAG HPTLC system equipped with Linomat V sample applicator, twin through plate development chamber, TLC scanner III and integration software WIN CATS-4.02. Physalis peruviana fruit extract was tested for phytochemical screening and in vitro anti-oxidant enzymes like 2, 2-diphenyl-1-picryl hydrazyl radical (DPPH), total antioxidant activity and reducing ability. Physalis peruviana fruit extract effectively scavenged free radicals at all different concentrations and showed its potent antioxidant activity. Phytochemical analysis revealed the presence of various major phytoconstituents like alkaloids, flavonoids, saponins, phenolics, tannins and anthraquinones. The HPTLC fingerprint qualitatively revealed predominant amount of quercetin. Physalis peruviana fruit extract will be subjected to further extensive studies to isolate and identify their active constituents which are useful for understanding their mechanism of action as antioxidants.

Potential hepatic-protective effect of Physalis peruviana against lead-induced toxicity in albino rats

“…The ethanol extract had the highest scavenging activity (67.60%), followed by the methanol extract (54.21%), and the water extract had the lowest (34.14%) as presented in Figure 4. In a study conducted by Saratha et al [12], the inhibition percentages of the ethanolic extract of Physalis peruviana fruit from 14.5 at 50 μg/Ml to 31.7 at 250 μg/mL were shown to be lower compared to the results of the current study. The DPPH assay results show that the extract of Physalis peruviana fruit possesses free radical scavenging activity.…”

“…However, some studies have used different methods to determine the antioxidant potency of Physalis peruviana fruit, implying that the fruit has good antioxidant activity. Differences in technique, ripeness level, or extraction method could account for the disparity in scavenging values across studies [12].…”