Evaluation of In Vitro Chromosome-doubled Regenerates with Resistance to Potato Tuber Moth (Phthorimaea opercullella (Zeler)).

Watanabe, Junko; Orrillo, Matilde; Watanabe, Kazuo

doi:10.5511/plantbiotechnology.16.225

Cited by 6 publications

(5 citation statements)

References 27 publications

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“…The second phase is that shoots form, the callus was changed into the MSA medium (CIP-Centro Internacional de la Papa-International Potato Center): Murashige Minimal Organic Medium with a low concentration of GA3 and sucrose. Interestingly, these tetraploid potatoes are more likely to be resistant to potato tuber moths [12,37,38] . In micropropagation of tomato (Solanum lycopersicum) induced by IAA and zeatin, the frequency of polyploid found ranging 9%-14% is an apparent example [39] .…”

Section: Achievements Of Polyploidization Through Plant Growth Regula...mentioning

confidence: 99%

“…However, using antimitotic chemicals might make a high ratio of chimera plants which reached 50%-100% by treating oryzalin [8,10] . In contrast, the method of phytohormone induction that can make euploidy plants with chromosomes doubling by phytohormone-based induction to be substantially successful [11,12] will be deeply discussed here. Although studies showed that residues of phytohormones when using in production or in the field can affect environmental pollution, the development of animal, human health problems, and food poisoning if using them was not corrected the producer's instruction [13,14] .…”

Section: Introductionmentioning

confidence: 99%

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Utilizing method of plant growth regulators induction in processing of polyploidization—A perspective crop development

Hieu

2023

Trends Geneti. Evolu.

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Nowadays, it seems like human beings are worried about how the world will meet its food security demand urgently when they have faced on rapidly increasing population and combat climate change. Scientists and researchers are indispensably investigating to enhance food sources such developing novel cops with high yield and good quality and even coping with the adverse environment in terms of biotic and abiotic stresses. Thus, there is one valuable method it is believed that should be developed further. In fact, it is believed that human beings should continue using it as soon as possible because it can provide the world with a source of food. Polyploidization introduced by plant growth regulator induction is a good method because it is safety and easy to develop new crops with potential agronomic traits, these polyploidy plants are rare aneuploid and it contains intriguing characteristics of polyploidy plants in adapting to ecological variability. This review sheds light on 1) summarizing molecular mechanism of plant growth regulator induction for plant ploidy manipulation; 2) achieving of polyploidization through plant growth regulator induction; 3) enumerating the perspectives of polyploidization in crop development to cope with climate change. Although the role of phytohormones is underestimated, the effectiveness on physiological level of plants to make polyploidy plants is worth considering and the effects and bio-safety of that on human are also concerned.

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Section: Achievements Of Polyploidization Through Plant Growth Regula...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Utilizing method of plant growth regulators induction in processing of polyploidization—A perspective crop development

Hieu

2023

Trends Geneti. Evolu.

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“…The ploidy level of the original diploid plant and tetraploid plants was confirmed by chromosome count. Root tips were excised and collected from in vitro plants to chromosome counting according to a method previously described by Watanabe et al (1999). The samples were then immersed in 0.5% (w/v) colchicine solution for 6 h. After washing with distilled water, the root tips were treated with 1 N hydrochloric acid (HCl), incubated at 60 °C for 8 min.…”

Section: Chromosome Countingmentioning

confidence: 99%

Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Mohammadmehdi

Shukurova

Watanabe

2021

In Vitro Cell.Dev.Biol.-Plant

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Polyploidization can be a way to produce new varieties in vegetatively propagated species where options on increasing genetic variability are limited compared with sexual reproduction. While there are hundreds of publications with in vitro methods in somatic doubling, it is cardinal to custom-test for a target species of interest on choosing specific reagents and optimizing conditions. This research was performed to provide a reference process for Zingiberaceae species of which the majority is reproduced with vegetative propagation. Ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig, were employed to optimize chromosome doubling for tetraploid production as they are typically used by vegetative propagation. However, they have the popularity as ornamentals globally due to their horticultural aspects. Tetraploid induction was optimized by in vitro somatic chromosome doubling on H. coronarium and H. gardnerianum through callogenesis. The explant segments of young leaf blades or leaf sheaths were treated with different concentrations of either with colchicine or with oryzalin. The regenerated shoots from callus cultures were transferred to basal MS medium for 2mo to confirm somatic stability. The ploidy was assessed by flow cytometry, measuring the size and density of stomata, counting chromosomes, and chloroplasts in guard cells. The highest percentage of tetraploid regenerated plants was observed with 1250 µM colchicine treatment for Hedychium gardnerianum when compared to the other treatments tested, while no tetraploid plants were obtained from the oryzalin treatment. In H. coronarium, four mixoploid regenerated plants treated with 15 µM oryzalin were confirmed and no shoots were obtained from the colchicine treatment. The results indicated that the in vitro polyploid induction is optimized with the two Hedychium species, which could reference other Zingiberaceae species.

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“…Among them, Mexican S. bulbocastanum and S. cardiophyllum may represent a useful source of noteworthy genes. Resistance to a number of pests and diseases has been reported by Watanabe et al (1999), Bamberg et al (1994), andChen et al (2003). Due to post-zygotic incompatibility isolation mechanisms, these species have not been used extensively in potato breeding.…”

Section: Introductionmentioning

confidence: 95%

Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes

Aversano

Ercolano

Frusciante

et al. 2007

Genet Resour Crop Evol

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Worldwide, a variety of pathogens negatively affect potato production, resulting in an estimated 22% annual yield reduction. Wild Solanum species represent a unique gene pool where all the traits necessary to improve the cultivated potato can be found. Therefore, breeding efforts for improved disease resistance and research aimed at characterizing wild germplasm have been extensively made. In this paper, sources of resistance to Phytophthora infestans, Erwinia carotovora subsp. carotovora, Fusarium solani and Globodera spp. have been investigated in several clones of two Solanum species originating from Central Mexico (S. bulbocastanum and S. cardiophyllum). Interestingly, we found sources of combined resistance to late blight and bacterial soft rot. This is an important finding considering that the development of resistant potato varieties has been hindered by the scarcity of resistant germplasm. In addition, we explored molecular differences within and between the two species generating AFLP fingerprints. By means of six primer pair combinations, we found 13 and 16 putative species-specific AFLP markers for S. bulbocastanum and S. cardiophyllum, respectively, and a bounty of markers useful for mapping, MAS, and cloning purposes. The phenotypic and molecular information associated to S. bulbocastanum and S. cardiophyllum for designing strategies of assisted selection are discussed.

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Evaluation of In Vitro Chromosome-doubled Regenerates with Resistance to Potato Tuber Moth (Phthorimaea opercullella (Zeler)).

Cited by 6 publications

References 27 publications

Utilizing method of plant growth regulators induction in processing of polyploidization—A perspective crop development

Utilizing method of plant growth regulators induction in processing of polyploidization—A perspective crop development

Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes

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