Evaluation of in vitro cytotoxic activity of mono-PEGylated StAP3 (Solanum tuberosum aspartic protease 3) forms

Muñoz, Fernando Felipe; Caracciolo, Pablo C.; Daleo, Gustavo Raúl; Abraham, Gustavo Abel; Guevara, María Gabriela

doi:10.1016/j.btre.2014.05.007

Cited by 2 publications

(1 citation statement)

References 78 publications

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“…The proteases isolated from Solanum tuberosum is an example, since, some participate in plant-microbe interactions to mediate defense mechanisms (Frey et al, 2018). Therefore some of them have the antimicrobial effect (Muñoz et al, 2014). Another example is the dioscorine since it regulates the expression of some cytokines (Hsu et al, 2013), while lunasin from Solanum nigrum is effective against cancer (Alaswad and Krishnan, 2016).…”

Section: Introductionmentioning

confidence: 99%

CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.

et al. 2019

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Solanum marginatum L. f. (Solanaceae) is used in traditional Mexican medicine to treat infectious diseases and cancer, although lacks of the scientific validation. Consequently, we proposed a study of the enzymatic, antioxidant, antibacterial and toxic activities of an S. marginatum L. f. protein fraction previously characterized. For this, a protein extract from leaves of S. marginatum L. f. obtained and the protein fraction separated by FPLC. The protein content was quantified by the bicinchoninic acid (BCA) method and the enzymatic activity measured by the Kunitz method. Subsequently, protein subfractions were identified by SDS-PAGE. Additionally, the ability to capture radicals by the DPPH and ABTS methods was determined. The toxic effect was assessed with the A. salina test while cell viability in peripheral blood mononuclear cells (PBMC) tested by MTT method. Isolation of PF-FPLC was carried out at tr = 2.13 min, further, separated and identified seven subfractions with molecular weights ranging from 18-112 kDa, while the optimal conditions of enzymatic activity were 37°C, pH 7 and 120 min. The ABTS and DPPH methods showed a radical capture percentage of 20 and 62% respectively. The PF-FPLC proteins showed no toxicity in A. salina. However, the PF-FPLC showed a low antimicrobial effect (inhibition ≤ 35%), and decreases PBMC viability.

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Section: Introductionmentioning

confidence: 99%

CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.

et al. 2019

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Discriminative study of a potato (Solanum tuberosum L.) cultivation region by measuring the stable isotope ratios of bio-elements

Chung

Kim

Jin³

et al. 2016

Food Chemistry

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Evaluation of in vitro cytotoxic activity of mono-PEGylated StAP3 (Solanum tuberosum aspartic protease 3) forms

Abstract: HighlightsSelective StAP3 cytotoxic activity is not affected by PEGylation.mono-PEGylated StAP3 forms exhibit higher antifungal activity than the native protein.PEGylation improves StAP3 cytotoxicity against Gram-positive bacteria.

Cited by 2 publications

References 78 publications

CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.

CARACTERIZACIÒN PARCIAL DE UNA FRACCIÓN PROTEICA POTENCIALMENTE BIOACTIVA DE Solanum marginatum L. f.

Discriminative study of a potato (Solanum tuberosum L.) cultivation region by measuring the stable isotope ratios of bio-elements

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