Evaluation of In Vitro Cytotoxicity of Heat-cure Denture Base Resin Processed with a Dual-reactive Cycloaliphatic Monomer

Abstract

Aim:The aim of this study is to evaluate cytotoxicity of tricyclodecane dimethanol diacrylate (TCDDMDA) when added to conventional heatcure methyl methacrylate (MMA) monomer at 10% and 20% (v/v) concentrations. Materials and methods: Twenty seven disk-shaped processed specimens were divided into control group (n = 9; comprises specimens made without substituting TCDDMDA in MMA) and two experimental groups (n = 9 each; specimens prepared by substituting TCDDMDA in MMA at 10% and 20% (v/v) concentration). Eluates were prepared by placing three specimens of each group into 9 mL of culture medium and then incubated at 37ºC for 24 hours. Continuous cells lines of L929 mouse fibroblast cells were used and MTT assay was employed to assess cytotoxicity. One-way analysis of variance (ANOVA) with post hoc Tukey's honestly significant difference (HSD) test was used to compare the mean optical density (OD) values and cell viability among the groups. Results: A statistically significant difference was obtained (p = 0.000) when the mean and standard deviation of OD and cell viability (%) of the groups were compared. Highest OD value and cell viability was obtained with E20 group followed by E10 group. Conclusion: Addition of TCDDMDA in MMA of heat-cure denture base resin has no cytotoxic effect on L929 mouse fibroblasts. Clinical significance: Dual-reactive TCDDMDA is a crosslinking monomer which has no cytotoxic effects on mammalian cell cultures. Hence, incorporation of TCDDMDA to MMA can be extrapolated and projected for fabricating dentures without compromising biocompatibility.