Evaluation of In Vitro Genotoxicity of Polystyrene Nanoparticles in Human Peripheral Blood Mononuclear Cells

Babonaitė, Milda; Čepulis, Matas; Kazlauskaitė, Jūratė; Lazutka, Juozas Rimantas

doi:10.3390/toxics11070627

Cited by 6 publications

(1 citation statement)

References 44 publications

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“…Both the in vivo and in vitro experiments indicate that NPs can penetrate cell membranes and can be internalized into cells, inducing intracellular biological effects. It has been shown that the cellular granularity (measured by flow cytometry side scatter) can be predictive of NP accumulation in cells [ 34 , 35 ]. The PET NPs of different stages of purity were tested for uptake by the CD14+ monocytes of human PBMCs.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Djapovic,

Apostolovic,

Postic

et al. 2023

Polymers

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Manufactured nanoplastic particles (NPs) are indispensable for in vitro and in vivo testing and a health risk assessment of this emerging environmental contaminant is needed. The high surface area and inherent hydrophobicity of plastic materials makes the production of NPs devoid of any contaminants very challenging. In this study, we produced nanoprecipitated polyethylene terephthalate (PET) NPs (300 nm hydrodynamic diameter) with an overall yield of 0.76%. The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. For a wide range of surfactant/NP ratios (17:100 to 1.2:100), the measured zeta potential changed from −42.10 to −34.93 mV, but with an NP concentration up to 100 μg/mL, no clear differences were observed in the cellular assays performed in protein-rich media on primary human cells. The remaining impurities contributed to the outcome of the biological assays applied in protein-free buffers, such as human red blood cell hemolysis. The presence of SDS increased the NP-induced hemolysis by 1.5% in protein-rich buffer and by 7.5% in protein-free buffer. As the size, shape, zeta potential, and contaminants of NPs may all be relevant parameters for the biological effects of NPs, the relative quantification of impurities exemplified in our work by the application of 1H NMR for PET NPs and the ionic surfactant SDS could be a valuable auxiliary method in the quality control of manufactured NPs.

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Section: Resultsmentioning

confidence: 99%