Evaluation of in vivo and in vitro anti-inflammatory activity of Ajuga bracteosa Wall ex Benth

Singh, Raghunath; Patil, SK .; Pal, Gagandeep; Ahmad, Mumtaz

doi:10.1016/s2222-1808(12)60191-1

Cited by 13 publications

(8 citation statements)

References 12 publications

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“…(Family Labiateae) is a perennial plant and is distributed widely in Kashmir and sub-Himalayan tract. It is recommended in Ayurveda to treat numerous ailments [ 17 – 19 ]. Medicinal properties of A. bracteosa , such as astringent, anthelmintic, anti-inflammatory and anti-microbial led its use into folk medicine [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Ajuga bracteosa for antioxidant, anti-inflammatory, analgesic, antidepressant and anticoagulant activities

Kayani¹,

Dilshad

Ahmed

et al. 2016

BMC Complement Altern Med

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BackgroundAjuga bracteosa has been extensively used traditionally for the treatment of a variety of diseases. The aim of the study was to scientifically validate the wide-scale exploitation of A. bracteosa in folk medicine various in vitro and in vivo assays. Moreover, these activities were related to the intrinsic biologically active phytoecdysteroids of A. bracteosa.MethodsAerial and root parts of A. bracteosa were first extracted separately with chloroform (AbCA and AbCR) and the residue was again extracted with methanol (AbMA and AbMR). Total flavonoid and phenolic contents were assayed as quercetin (QE) and gallic acid equivalents (GAE), respectively. These extracts were analyzed for in vitro antioxidant assessment including DPPH and H2O2 (% inhibition of free radicals), and reducing power and phosphomolybdenum methods (ascorbic acid equivalents AAE mg/g DW). Further, these extracts were assayed in vivo in separate groups of Sprague–Dawley rats for carrageenan induced rat paw edema inhibition, hotplate antinociception, forced swim antidepression and anticoagulation. Dose of each crude extract and standard drug given to rats was 200 mg/Kg- and 10 mg/10 mL/Kg body weight respectively. Plant extracts and standard drugs were administered orally, 60 min prior to the conduction of assays. Moreover, biologically active phytoecdysteroids were screened in A. bracteosa with the help of RP-HPLC.ResultsAbMA represented highest values of flavonoids (QE 1.98 % DW) and phenolic contents (GAE 5.94 % DW), significantly scavenged DPPH radicles (IC50 36.9) and reduced ferric ions with 718.4 mg ascorbic acid equivalent/g (AAE). Highest total antioxidant capacity was expressed by AbMR (927 mg AAE) with an IC50 value 19.1 μg/mL. The extracts which were found potent anti-oxidants, were also good at in vivo activities. AbMA significantly reduced edema in all the three hours of treatment (67.9, 70.3 and 74.3 %). AbMA also showed maximum nociceptor suppression in analgesic assay by delaying the time to start licking of paws in rats (57.7 ± 4.9 s). In addition, maximum anti-coagulation was also exhibited by AbMA (89.3 s), while all extracts were found strong antidepressants (≤15.66 s immobility time). Screening of biologically active phytoecdysteroids revealed the presence of 20-hydroxyecdysone (20-HE), makisterone (MKA), cyasterone (CYP) and ajujalactone (AJL). Total phytoecdysteroid content found in A. bracteosa was 1232.5 μg/g DW and 20-HE was most abundant (1232.5 μg/g DW) as compared to other phytoecdysteroids.ConclusionBased on the tested in vitro and in vivo activities, AbMA was found to be a promising bioactive extract. These activities can be attributed to the intrinsic polyphenols and phytoecdysteroids contents of A. bracteosa.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Ajuga bracteosa for antioxidant, anti-inflammatory, analgesic, antidepressant and anticoagulant activities

Kayani¹,

Dilshad

Ahmed

et al. 2016

BMC Complement Altern Med

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“…They were previously reported to be present in few genera of Lamiaceae such as Scutellaria, Stachy and Ajuga. Regarding the flavonoids, the O-glycosides of apigenin and luteolin account for the major flavonoidal constituents of the genus Ajuga, while C-flavonoid glycosides such as isovitexin and orientin were rarely encountered (Israili and Lyoussi 2009;Singh et al 2012). It is noteworthy that isoorientin was found as one of the major constituents of A. laxmannii in this study.…”

Section: Discussionmentioning

confidence: 58%

“…Some Ajuga species, such as A. remota, is traditionally utilized as an herbal remedy for the treatment of fever, infections, malaria as well as tuberculosis (Israili and Lyoussi 2009;Cocquyt et al 2011). Hypoglycaemic, anti-arthritic, antioxidant, antimicrobial, anti-fungal and anti-inflammatory effects of Ajuga species have also been reported in previous studies (Kariba 2001;El-Hilaly and Lyoussi 2002;El-Hilaly et al 2006;Kaithwas et al 2012;Singh et al 2012;Ben Mansour et al 2013;Hsieh et al 2014). The genus Ajuga is represented by 11 species in the flora of Turkey (Davis 1982).…”

Section: Introductionmentioning

confidence: 85%

Evaluation of in vitro antiprotozoal activity of Ajuga laxmannii and its secondary metabolites

Atay

Kırmızıbekmez

Kaiser

et al. 2016

Pharmaceutical Biology

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Context Some Ajuga L. (Lamiaceae) species are traditionally used for the treatment of malaria, as well as fever, which is a common symptom of many parasitic diseases. Objective In the continuation of our studies on the identification of antiprotozoal secondary metabolites of Turkish Lamiaceae species, we have investigated the aerial parts of Ajuga laxmannii. Materials and methods The aerial parts of A. laxmannii were extracted with MeOH. The H 2 O subextract was subjected to polyamide, C 18 -MPLC and SiO 2 CCs to yield eight metabolites. The structures of the isolates were elucidated by NMR spectroscopy and MS analyses. The extract, subextracts as well as the isolates were tested for their in vitro antiprotozoal activities against Plasmodium falciparum, Trypanasoma brucei rhodesiense, T. cruzi and Leishmania donovani at concentrations of 90-0.123 mg/mL. Results Two iridoid glycosides harpagide (1) and 8-O-acetylharpagide (2), three o-coumaric acid derivatives cis-melilotoside (3), trans-melilotoside (4) and dihydromelilotoside (5), two phenylethanoid glycosides verbascoside (6) and galactosylmartynoside (7) and a flavone-C-glycoside, isoorientin (8) were isolated. Many compounds showed moderate to good antiparasitic activity, with isoorientin (8) displaying the most significant antimalarial potential (an IC 50 value of 9.7 mg/mL). Discussion and conclusion This is the first report on the antiprotozoal evaluation of A. laxmannii extracts and isolates. Furthermore, isoorientin and dihydromelilotoside are being reported for the first time from the genus Ajuga.ARTICLE HISTORY

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“…Some of these drugs such as aspirin, diclofenac, ketorolac, naproxen, and piroxicam have toxic effects such as risk of gastrointestinal bleeding. 7,8 Moreover, the generation of oxygen free radicals is known to be involved in the development of the inflammatory process. These radicals are highly reactive molecules with an unpaired electron that can initiate radical chain reactions, leading to damage or destruction of the normal function of a living cell, and consequently causing many different diseases such as neurodegenerative disorders, cancer, cardiovascular diseases, atherosclerosis, diabetes, cataracts, and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Anti-Inflammatory Activity of Some <i>Lathyrus</i> L. (Fabaceae) Species Growing in Turkey

Heydari¹,

Saltan²,

Eryılmaz³

et al. 2019

tjps

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In vitro anti-enflamatuvar etki ise insan kırmızı kan hücresi kullanarak membran stabilizasyon yöntemi ile değerlendirilmiştir. Bulgular: Etil asetatlı fraksiyonlar diğer ekstre ve fraksiyonlara göre daha yüksek antimikrobiyal aktivite, sulu fraksiyonlar ise diğer ekstre ve fraksiyonlara göre daha yüksek anti-enflamatuvar aktivite göstermiştir. Ayrıca, L. pratensis'in su fraksiyonu, standart olarak kullanılan asetilsalisilik asit ve diklofenak sodyumdan daha yüksek anti-enflamatuvar aktivite göstermiştir. Sonuç: Elde edilen sonuçlara göre Lathyrus türlerinin ekstreleri ve fraksiyonlarının membran stabilizasyon aktiviteye sahip olup, ve in vivo antienflamatuvar aktivite deneyleri için bir ön çalışma olabileceğini belirtmiştir. Anahtar kelimeler: Anti-enflamatuvar aktivite, antimikrobiyal aktivite, insan kırmızı kan hücresi membran, Lathyrus

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Evaluation of in vivo and in vitro anti-inflammatory activity of Ajuga bracteosa Wall ex Benth

Cited by 13 publications

References 12 publications

Evaluation of Ajuga bracteosa for antioxidant, anti-inflammatory, analgesic, antidepressant and anticoagulant activities

Evaluation of Ajuga bracteosa for antioxidant, anti-inflammatory, analgesic, antidepressant and anticoagulant activities

Evaluation of in vitro antiprotozoal activity of Ajuga laxmannii and its secondary metabolites

Antimicrobial and Anti-Inflammatory Activity of Some <i>Lathyrus</i> L. (Fabaceae) Species Growing in Turkey

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