Evaluation of in vivo and in vitro repopulation assays for murine haemopoietic stem cells

Sluijs, J.P. van der; Baert, M.R.M.; Beurden, C.A.J. van der Linden-van; Ploemacher, Rob E.

doi:10.1007/bf00426164

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…Previous experiments indicated that, although NA cells from LTBMC retained colony-forming potential, stem cell activity was rapidly lost from culture (29). Certain stromal cell lines maintain HSC activity for up to 5 weeks in vitro, but the expansion of HSC reported was modest (30).…”

Section: Discussionmentioning

confidence: 99%

Sustainedex vivoexpansion of hematopoietic stem cells mediated by thrombopoietin

Yagi

Ritchie

Sitnicka

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

121

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The hematopoietic stem cell (HSC) is defined as a cell that can either self-replicate or generate daughter cells that are destined to commit to mature cells of different specific lineages. Self-replication of the most primitive HSC produces daughter cells that possess a long (possibly unlimited) clonal lifespan, whereas differentiation of HSC produces daughter cells that demonstrate a progressive reduction of their clonal lifespan, a loss of multilineage potential, and lineage commitment. Previous studies indicated that the proliferation of HSC ex vivo favors differentiation at the expense of self-replication, eventually resulting in a complete loss of HSC. In contrast, transplantation studies have shown that a single HSC can repopulate the marrow of a lethally irradiated mouse, demonstrating that self-renewal of HSC occurs in vivo. Thrombopoietin (TPO) has been shown to function both as a proliferative and differentiative factor for megakaryocytes and as a survival and weakly proliferative factor for HSC. Our studies focused on the effects of exogenous TPO on HSC in mouse long-term bone marrow cultures (LTBMC). Previous results indicate that HSC decline in LTBMC in the absence of TPO. In contrast, the continuous presence of TPO resulted in the generation of both long-and short-term repopulating HSC as detected by an in vivo competitive repopulation assay. HSC were generated over a 4-month period at concentrations similar to normal bone marrow. Our results demonstrate that TPO can mediate the self-replication of HSC in LTBMC, and provide proof that HSC can self-replicate ex vivo.

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Section: Discussionmentioning

confidence: 99%

Sustainedex vivoexpansion of hematopoietic stem cells mediated by thrombopoietin

Yagi

Ritchie

Sitnicka

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

121

View full text Add to dashboard Cite

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“…Therefore, the lineage of these immature cells could not be ascertained. At present there is no specific receptor that strictly distinguishes granulocytes from monocytes of such a degree of immaturity as the cells observed in this study, since those cells share a 456 supposed common bone marrow pluripotential progenitor (22,41). Some researchers have utilized cytochemical methods for non-specific esterase and chloroacetate esterase for discriminating monocytic from myeloid progenies (37).…”

Section: Discussionmentioning

confidence: 76%

Tumor necrosis factor α plus interleukin 1β treatment protects granulocytopenic mice fromPseudomonas aeruginosalung infection: Role of an unusual inflammatory response

Amura

Fontán

Sanjuán

et al. 1995

APMIS

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We have recently demonstrated that treatment with interleukin 1β (IL‐1β) plus tumor necrosis factor a (TNFα) protects granulocytopenic hosts from Pseudomonas aeruginosa aerosol challenge. In this study we characterized the inflammatory response induced by P. aeruginosa in granulocytopenic mice treated with 2,000 U IL‐1β plus 2,000 U TNFα. Treatment with the nonsteroidal anti‐inflammatory agent piroxicam abolished both the protective effect of cytokine treatment and the increase in myeloperoxidase (MPO) pulmonary activity. Histopathological studies revealed that, after aerosol challenge with P. aeruginosa, treatment with these cytokines induced migration and extravasation of mononuclear cells of immature appearance into the lung parenchyma. These cells contained MPO in their cytoplasm and displayed phagocytic capacity. Resident alveolar macrophages exhibited signs of activation and appeared in reduced numbers in bronchoalveolar lavage fluid. We suggest that the inflammatory response promoted by low TNFα plus IL‐1β doses may be one mechanism responsible for protection of granulocytopenic hosts from P. aeruginosa aerosol challenge.

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“…Traditionally, it has been exceedingly difficult to maintain or expand robust levels of transplantable hematopoietic activity in any in vitro culture system. A general observation is that hematopoietic cultures may support the proliferation of stem cells but only in concert with differentiation events (27,28). It would be surprising indeed if the conditions employed in the muscle cultures (which only include fetal calf serum and chick embryo extract) could support bona fide hematopoietic stem cells isolated from bone marrow.…”

mentioning

confidence: 99%

The power of stem cells reconsidered?

Lemischka

1999

Proc. Natl. Acad. Sci. U.S.A.

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Evaluation of in vivo and in vitro repopulation assays for murine haemopoietic stem cells

Cited by 5 publications

References 20 publications

Sustainedex vivoexpansion of hematopoietic stem cells mediated by thrombopoietin

Sustainedex vivoexpansion of hematopoietic stem cells mediated by thrombopoietin

Tumor necrosis factor α plus interleukin 1β treatment protects granulocytopenic mice fromPseudomonas aeruginosalung infection: Role of an unusual inflammatory response

The power of stem cells reconsidered?

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