Evaluation of in Vivo Diuretic Activity of Methanolic Extracts of Clutia Abyssinica (Euphorbiaceae) Roots in Wistar Albino Rats

Tegegne, Abebaw; Mishra, Bharat; Geta, Mestayet

doi:10.24087/iam.2017.1.8.215

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…The delayed diuresis onset with the HME and its EAF and CF may be ascribed due to the slow absorption properties of the responsible active ingredient(s). The long diuresis duration of action with the HME and its EAF and CF throughout the study period (until the 24th hr) may be attributed due to the slow clearance properties of the responsible ingredient(s) [ 27 , 28 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae)

Welu

Yimer

Hailu

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Introduction. Clerodendrum myricoides (Lamiaceae) has been traditionally used for the treatment of various ailments, including body swelling and urine retention. The present study aimed to evaluate the diuretic activity of a crude extract and solvent fractions of the root bark of C. myricoides. Methodology. The coarsely powdered root bark of C. myricoides was extracted by a cold maceration method using 80% methanol. A portion of the extract was fractionated based on the polarity index of solvents to obtain chloroform, ethyl acetate, and aqueous fractions. To investigate the diuretic activity of the plant, rats were divided into fifteen groups. The normal control groups received either water or 2% tween 80, the standard group received furosemide (10 mg/kg), and the test groups were administered the hydromethanolic extract and solvent fractions at the doses of 100, 200, and 400 mg/kg by the oral route. The urine volume, urine pH, urine, and serum electrolytes were determined and compared with the standard and normal control groups. Results. The crude hydromethanolic extract, ethyl acetate, and chloroform fractions induced significant diuresis at a dose of 400 mg/kg ( P < 0.001 ) compared to the aqueous fraction. The hydromethanolic extract at 200 mg/kg and 400 mg/kg also caused noticeable diuresis ( P < 0.001 ) compared to the standard, furosemide. Rats treated with hydromethanolic extract, ethyl acetate, and chloroform fractions showed delayed onset and prolonged diuresis in a dose-dependent fashion compared to the aqueous fraction ( P < 0.05 ). The hydromethanolic extract and solvent fractions produced the highest saliuretic and natriuretic index compared to the standard, furosemide. The crude hydromethanolic extract also failed to produce any sign of toxicity up to 2000 mg/kg. Conclusion. From this study, the hydromethanolic extract and ethyl acetate fraction of the root bark of C. myricoides produced a prominent diuretic effect in rats.

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Section: Discussionmentioning

confidence: 99%

In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae)

Welu

Yimer

Hailu

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…10,11 Both the roots and leaves are used in the treatment of venereal and skin diseases, chest problems, cancer and fertility in humans. 12 Some scientific studies revealed its in vivo antimalarial, 13 anti-trypanosomal, 14 analgesic, 15 antiinflammatory, 16 antipyretic, 17 diuretic 18 and in vitro antioxidant and anti-proliferative 19 activities. The medicinal value of Clutia abyssinica may be due to its constituents that produce a definite physiological action.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Hepatoprotective Activity of the Crude Extract and Solvent Fractions of Clutia abyssinica (Euphorbiaceae) Leaf Against CCl4-Induced Hepatotoxicity in Mice

Meharie¹,

Amare²,

Belayneh³

2020

JEP

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Background: Liver is a vital organ that plays a major role in the elimination of xenobiotics from the body. Diseases that affect the liver become major health problems and challenge health-care professionals as well as the pharmaceutical industry. Since the conventional treatment of liver diseases is associated with a wide range of adverse effects, botanical agents are commonly used. Among these agents, Clutia abyssinica is the most widely used herb in Ethiopian traditional medicine. Objective: To evaluate the hepatoprotective activity of the crude 80% methanol extract and solvent fractions of Clutia abyssinica leaves in mice. Methods: The leaves of Clutia abyssinica were extracted by cold maceration using 80% methanol as a solvent, and the solvent fractions were obtained in liquid-liquid extraction with chloroform, n-butanol and distilled water. Male mice were treated with the vehicles (distilled water or 2% Tween 80), three different doses (100, 200 and 400 mg/kg) of the crude 80% methanol extract and three solvent fractions, the standard drug (silymarin 100 mg/ kg), and the hepatotoxicant carbon tetrachloride (CCl 4). Then, the levels of biomarkers of liver injurysuch as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP)and liver function such as total protein, albumin, and bilirubin were measured. Evaluation of the change in body weight and liver weight, histopathologic examination and in vitro antioxidant assay against CCl 4-induced hepatotoxicity were also carried out. Results: The 80% methanol extract decreased the absolute and relative weight of the liver of mice at the doses of 200 and 400 mg/kg (p<0.01 and p<0.001, respectively). It also suppressed the plasma levels of AST, ALT and ALP (p<0.001) in the aforementioned doses. Among fractions, the n-butanol fraction showed maximum hepatoprotective activity in its dose of 200 and 400 mg/kg (p<0.001, in all cases). Likewise, the chloroform fraction (400 mg/kg) reduced to a similar extent (p<0.001 in all cases). In stark contrast, the aqueous fraction failed to affect the levels of all biomarkers of hepatocyte injury. The crude methanol extract and n-butanol fraction were able to return the normal hepatic architecture of hepatocytes and scavenge free radicals in the 1,1-diphenylpicrylhydrazyl (DPPH) assay. Conclusion: Clutia abyssinica is endowed with hepatoprotective activity, probably mediated via its antioxidant activity. Thus, Clutia abyssinica can be taken as one candidate for the development of hepatoprotective agents because of its good safety profile.

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“…( 25) The CAI effect is disregarded if the Cl -/Na + +K + ratio is between 0.8 and 1.00, but if it is less than 0.8, it is considered to have strong CAI activity. (30) In this study, both M400 and EAF400 exhibited a strong CAI effect with a value of 0.66 and 0.68, respectively. This suggests that M400 and EAF400 might possibly have an inhibiting effect on the renal tubules' carbonic anhydrase enzyme.…”

Section: Discussionmentioning

confidence: 51%

Evaluation of the Diuretic Activities of 80% Methanol Leaf Extract and Solvent Fractions ofRumex nepalensisin Mice

Tafesse,

Asrie,

Belete

2023

Preprint

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BackgroundThe leaf ofRumex nepalensishas historically been employed to treat urinary retention and as a diuretic. Despite these assertions, there has been very little research addressing the diuretic effect of the leaves ofR. nepalensis. Therefore, this study was done to examine the diuretic properties of R. nepalensis leaves.MethodsCold maceration with 80% methanol was used to extract the coarsely powdered leaves ofR. nepalensis. The extract was separated using increasing polarity solvents, beginning with n-hexane, ethyl acetate, and water. Mice were split into sections to test the plant’s diuretic properties. The negative control groups were given either distilled water or 2% tween 80; the positive control groups were given furosemide (10 mg/kg); and the test groups were given the 80% methanol extract and solvent fractions orally at dosages of 100, 200, and 400 mg/kg. The urine volume was determined, and urine analysis was performed on each extract.ResultsAt dosage levels of 200 mg/kg and 400 mg/kg, the 80% methanol extract, ethyl acetate fraction, and aqueous fraction all produced substantial diuresis (p<0.001) as compared to the negative control. Similarly, mice given the 80% methanol extract, ethyl acetate fraction, and aqueous fraction demonstrated substantial natriuresis (p<0.001) and kaliuresis (p<0.001) at dosages of 200 mg/kg and 400 mg/kg, respectively, in comparison to the negative control.ConclusionThe diuretic activity ofR. nepalensiswas significantly seen in the 80% methanol extract, ethyl acetate, and aqueous fractions, which corroborated the plant’s traditional use.

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Evaluation of in Vivo Diuretic Activity of Methanolic Extracts of Clutia Abyssinica (Euphorbiaceae) Roots in Wistar Albino Rats

Cited by 3 publications

References 7 publications

In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae)

In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae)

<p>Evaluation of Hepatoprotective Activity of the Crude Extract and Solvent Fractions of <em>Clutia abyssinica</em> (<em>Euphorbiaceae</em>) Leaf Against CCl<sub>4</sub>-Induced Hepatotoxicity in Mice</p>

Evaluation of the Diuretic Activities of 80% Methanol Leaf Extract and Solvent Fractions ofRumex nepalensisin Mice

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