Evaluation of In Vivo Wound-Healing and Anti-Inflammatory Activities of Solvent Fractions of Fruits of Argemone mexicana L. (Papaveraceae)

Abstract

Introduction. The solvent fractions of the fruits of Argemone mexicana L. (Papaveraceae) have not yet been explored scientifically for in vivo wound healing and anti-inflammatory activities. The objective of this study was, therefore, to evaluate in vivo wound healing and anti-inflammatory activities of the solvent fractions of the fruit of Argemone mexicana L. (Papaveraceae) in rats. Method. The crude extract of Argemone mexicana was fractionated with n-hexane, ethyl acetate, and distilled water. Wound healing activity was evaluated using excision and incision wound models while anti-inflammatory activity was evaluated using carrageenan-induced rat paw and cotton pellet-induced granuloma models. The fractions were evaluated at 5 and 10% ointments using moist-exposed burn ointment as the standard drug, and 100, 200, and 400 mg/kg test doses using aspirin, and dexamethasone as standard drugs for wound healing and anti-inflammatory activities, respectively. All treatment administrations were made orally for anti-inflammatory activity and applied topically for wound healing activity. Result. The 10% w/w ethyl acetate fraction ointment showed a significant percentage of wound contraction, reduced period of epithelialization, increased amount of fibrosis, neovascularization, and collagen tissue formation ( p < 0.01 ). The ethyl acetate fraction also showed a significant increase in tensile strength (55%; p < 0.01 ) and (81.10%; p < 0.01 ) at the tested doses of 5 and 10% w/w ointments, which was comparable to moist-exposed burn ointment. The ethyl acetate fraction also revealed a significant percent edema inhibition (61.41%; p < 0.01 ), suppression of the exudate (38.09% p < 0.01 ), and granuloma mass formations (53.47% p < 0.01 ) at the tested dose of 400 mg/kg. Conclusion. The results of this study showed that the Ethyl acetate fraction of Argemone mexicana fruit has significant wound healing and anti-inflammatory activities which support the traditional claims of the experimental plant.

Evaluation of Wound-Healing Activity of Hydrogel Extract of Sansevieria trifasciata Leaves (Asparagaceae)

“…For instance, alkaloids have been reported to stimulate the formation of epithelial cells, expediting the re-epithelization process [35]. In addition, favonoids and polyphenols have been observed to possess antioxidant, anti-infammatory, and antimicrobial activities, which collectively contribute to the acceleration of wound healing [36][37][38][39]. Saponins have also been shown to contribute to wound contraction and enhance epithelialization [39,40].…”

“…In addition, favonoids and polyphenols have been observed to possess antioxidant, anti-infammatory, and antimicrobial activities, which collectively contribute to the acceleration of wound healing [36][37][38][39]. Saponins have also been shown to contribute to wound contraction and enhance epithelialization [39,40]. Triterpenoids possess astringent and antimicrobial properties, thus promoting wound healing and preventing infection [41,42].…”

Traditional uses, phytochemistry, pharmacological significance and toxicology of Argemone mexicana L. – A review approach

“…The application of the ointment resulted in a remarkable decrease in wound size, faster epithelialization, and elevated levels of fibrosis, neovascularization, and collagen tissue formation. There was an increased degree of tensile strength, oedema inhibition, and granuloma mass development (50).…”

Phytochemical, Pharmacological and Antimicrobial Properties of the Tissue Extracts of Argemone spp.

“…Taxonomic classification was difficult. Twenty-four and thirty-two species were identified and distributed in North and South America, Hawaii, Australia, India, and various parts of Africa and Asia [1][2][3][4]. Argemone L. plants with stems, leaves, and spiny capsules family of plants from whose Argemone Mexicana belong to this genus widely distributed in Mexico and the United States and used in traditional medicine for various pathologies, including does not require special care, and is easy to obtain; however, it is essential to clarify that phytochemical knowledge about the genus Argemone spp is limited and the scientific literature is scarce, so it is pertinent to carry out studies on the phytochemical profile, antioxidant, toxicological and antimicrobial activity of the plant for agrochemical, pharmaceutical, and therapeutic industry Approximately forty alkaloids compounds belonging to six groups with isoquinoline bases have been isolated from plants of the genus Argemone; however, the genus Argemone has been little studied by botanists.…”

“…Argemone mexicana is a thorny plant that grows mainly in subtropical states [4,5]. The medicinal application is in the traditional Indian medicine system for skin diseases, fever, diarrhea, and dysentery.…”