Evaluation of Indoor Air for Bacteria Organisms and their Antimicrobial Susceptibility Profiles in a Government Health Institution

Wemedo, S. A.; Robinson, V. K.

doi:10.9734/jamb/2018/42833

Cited by 14 publications

(19 citation statements)

References 7 publications

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“…The antibiotic profile of the isolates and identified bacteria revealed that ciprofloxacin, ofloxacin, were more susceptible, e, they were considered as drugs of choice for infections caused by bacteria isolated in this study. Comparatively, the results of antibiotic profile tests against the isolates and bacteria reported in this study is in agreement with observations by Wemedo and Robinson [17] who reported the resistance of some bacterial isolates against Augmentin. The resistance of the microbes to Augmentin and Ceftazidime may be due to a regular abuse of the drugs against the isolates In vitro.…”

Section: Combined Antibiotic Sensitivity Pattern Of Isolates From Thesupporting

confidence: 93%

“…Combined Antibiotic Sensitivity pattern of Isolates from the five selected Vegetables study. Comparatively, the results of antibiotic profile tests against the isolates and bacteria reported in this study is in agreement with and Robinson [17] who reported the resistance of some bacterial isolates against Augmentin. The resistance of the microbes to Augmentin and Ceftazidime may be due to a regular abuse of the drugs against the…”

Section: Percentage Susceptibility Of the Isolates To Different Antibsupporting

confidence: 92%

“…Loop-full of 24-hour old pure culture of the test organisms from nutrient agar plates, were transferred into sterile diluents tubes and adjusted to 0.5 McFarland Turbidity Standard [16,17]. This was used as the standard inoculums.…”

Section: Preparation Of Standard Bacterial Inoculummentioning

confidence: 99%

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Characteristics and Antibiogram Studies of Bacteria Associated with Vegetables Stored In Raffia Baskets in Nigeria

Ogbonna

Douglas

Inana

2019

JALSI

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Raffia baskets are used in rural communities to preserve fresh vegetables for a given period of time. This method of storage is aimed at extending the shelf life of the vegetables until they are used. Thus, this study was aimed at determining the microbial succession during storage and susceptibility pattern of bacterial isolates from stored leafy vegetables to various antibiotics. Five (5) leafy vegetables namely, Bitter leaf (Vernomia anydalira), Water leaf (Talinum triangulare), Fluted pumpkin leaf, (Telfairia occidentalis), Okazi leaf (Gnetum africana) and Scent leaf (Ocimum gratissimum) were obtained from the Nigerian Stored Products Research Institute (NSPRI) farm, Port Harcourt. These vegetables were stored in a raffia basket for fourteen (14) days while another set of the vegetables were left in the open air as control. This set up was monitored for changes to occur. Standard microbiological techniques were employed for the various analyses of the vegetable samples. Both cultural and molecular characterizations of the isolates were done. The disc diffusion method was used in determining the susceptibility pattern of the bacterial isolates. The results of the predominant microorganisms identified were of the genus; Bacillus, Pseudomonas, Enterococcus, Enterobacter, Bordetella, Staphylococcus, Myroides, Escherichia, Serratia, Micrococcus and Acetobacter. Also, Bacillus species occurred in all the vegetables while Enterococcus faecalis, Acetobacter orientalis, Bordetella pertussis, Myroides xuanwuensis and Bacillus flexus were isolated on the third day of storage from the vegetables. The total heterotrophic bacterial counts ranged from 1.8 x106 cfu/g to 1.25 x107 cfu/g (bitter leaf), 9x105 cfu/g to 9.0 x106 cfu/g (Scent leaf), 7 x105 cfu/g to 1.88 x107 cfu/g (Okazi), 3.2x106 cfu/g to 1.05x107 cfu/g (Fluted pumpkin leaf) and 8.4 x106 cfu/g to 2.04 x107 cfu/g (water leaf). Antibiogram of bacterial isolates revealed that they were resistant to Augmentin and Ceftazidime according to Clinical Laboratory Standard Institute schemes. Combination of the antibiotics showed that most of the isolates were 100% susceptible to ciprofloxacin + erythromycin, and ciprofloxacin + ceftriaxone. The study revealed that for all the vegetables, those stored in the basket lasted longer and were fresher compared to those kept in the open space. This study revealed that the raffia baskets are suitable for storage and preservation of leafy vegetables, but microbial succession occurred as a result of increase in the period of storage leading to the proliferation of microorganisms. This could be from aerosols or by chance inoculation, through handling or materials used or other microorganisms which are inherent in the vegetables because of their contact with the soil or water used in the washing of the leaves.

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Section: Combined Antibiotic Sensitivity Pattern Of Isolates From Thesupporting

confidence: 93%

Section: Percentage Susceptibility Of the Isolates To Different Antibsupporting

confidence: 92%

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Characteristics and Antibiogram Studies of Bacteria Associated with Vegetables Stored In Raffia Baskets in Nigeria

Ogbonna

Douglas

Inana

2019

JALSI

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“…Twenty-four (24) hours old culture were aseptically introduced into 4 ml sterile normal saline, turbidity of the organism in the tube and was compared to the turbidity of the 0.5 McFarland Standard. Unto a sterile solid Muller Hinton agar plates the already standardized isolates were aseptically inoculated using sterile swab sticks and allowed to dry [11] after which antibiotic disc was aseptically placed on the solid media using sterile forceps. The inoculated plates were incubated at 37°C for 24 hours.…”

Section: Antibiotic Sensitivitymentioning

confidence: 99%

“…An antibiogram is a chart that displays the susceptibility test or responses of microorganisms against the antibiotics to which they were tested for [11]. Parkyz [12] posited that the antibiogram could be used as a guide by the clinicians and pharmacists towards choosing the most appropriate empiric antimicrobial treatment in the event of pending microbiology culture and susceptibility result.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Gram-negative Bacteria Isolated from Well Water in Ula-Ubie Community, Ahoada West, Nigeria

Nrior

Okpokiri

Akani

2020

MRJI

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Antibiotic resistance has become a great global problem. Thus, it has emerged as a public health challenge. The antibiotic susceptibility pattern of bacteria in well water was characterized with a view of determining the level of resistance in the environment. Fifty well water samples were collected from ten different points in Ula-Ubie community, Ahoda, Rivers State for a period of five months. Standard microbiological methods were used to analyse the population and types of bacteria in the water while methods recommended by the American Public Health Association (APHA) was used to determine the physicochemical parameters of the samples. The antibiotic susceptibility profile of the bacterial isolates was carried out using the disc diffusion methods. The total heterotrophic bacteria of the water samples ranged from 0.93±0.46 to 2.02±1.06 log 10 CFU/ml. The coliform counts ranged from 0.45±0.42 -2.55±2.33 log 10 CFU/ml, respectively. Despite the variations in the counts of the different bacterial population, there was no significant differences (P > 0.05) in the different well water samples. The physicochemical parameters except the pH were all within the permissible Nrior et al.; MRJI, 30(2): 1-10, 2020; Article no.MRJI.55043 2 limits. Klebsiella spp, Pseudomonas spp, Serratia spp and Enterobacter spp were identified in the well water. The pH of the water stations ranged from 4.66 to 5.80. The temperature ranged from 24.0 to 24.7. The electrical conductivity, salinity, dissolved oxygen, total hardness, alkalinity, total suspended solids, biochemical oxygen demand, nitrate, chloride, calcium and magnesium ranged from 22.9 -219, 0.03-0.13, 4.50-4.90, 5.00-22.0, ˂0.01-3.00, ˂0. 01, 49.6-84.5, 1.00-17.4, 3.00-24.5, 4.25-12.9 and 0,722-1.55 respectively. The antibiotic susceptibility profile showed that all the isolates were resistant to ceftazidime and augmentin, whereas Enterobacter spp were the most resistant bacteria amongst other bacterial genera to the antibiotics. Meanwhile there is an existence of multi-drug resistance. Thus, the wells could be considered not potable due to the presence of these bacterial isolates and the level of antibiotic resistant. Proper sanitation and cleanliness of well should be encouraged. Original Research Article

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Determination of drug-resistant bacteria in palmar surface and touchscreen cell phones from bystanders in an urban community

Campista-León

Uriel

Garcia-Guerrero

et al. 2022

Microbiological Research

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Evaluation of Indoor Air for Bacteria Organisms and their Antimicrobial Susceptibility Profiles in a Government Health Institution

Cited by 14 publications

References 7 publications

Characteristics and Antibiogram Studies of Bacteria Associated with Vegetables Stored In Raffia Baskets in Nigeria

Characteristics and Antibiogram Studies of Bacteria Associated with Vegetables Stored In Raffia Baskets in Nigeria

Prevalence and Antibiogram of Gram-negative Bacteria Isolated from Well Water in Ula-Ubie Community, Ahoada West, Nigeria

Determination of drug-resistant bacteria in palmar surface and touchscreen cell phones from bystanders in an urban community

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