Evaluation of inhibitory activity of natural plant polyphenols on Soybean lipoxygenase by UFLC-mass spectrometry

Zhang, Y.-Y.; Thakur, Kiran; Wei, Chao-Kun; Wang, H.; Zhang, J.-G.; Wei, Zhao‐Jun

doi:10.1016/j.sajb.2018.05.002

Cited by 20 publications

(11 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LOXs are dioxygenases that catalyse the oxidation of polyunsaturated fatty acids like linoleic and arachidonic acids and produce hydroperoxides. Immune, epithelial and tumour cells all express LOX enzymes which have a wide range of physiological activities, including skin disease, inflammation and carcin- ogenesis (42). Leukotrienes are important inflammatory mediators produced by the 5-LOX.…”

Section: Inhibition Type Of Lox Activitymentioning

confidence: 99%

Evaluation of the effect of Castanea sativa extracts on lipoxygenase activity

2022

View full text Add to dashboard Cite

Lipoxygenase LOX is a lipolysis enzyme that oxidises unsaturated fatty acids like arachidonic and linoleic acids to form unhealthy chemicals such as dienes, leukotrienes and malondialdehyde in advanced oxidation, which is harmful to cells. In this study, the polyphenols and saponins compounds were extracted from Castanea sativa using Soxhlet for 3 days. Colourimetric and HPLC techniques were used to identify polyphenols and saponins in the extracts respectively. The effects of these extracts were evaluated on LOX activity, which was purified from the liver of Iraqi Cows (AL-Sharabi Cows) as well as on E. coli and Pseudomonas bacteria resistance. According to the LOX purification procedures, the specific activity increased from 0.001 to 0.03 U/mg with a purification fold of 30 times and a yield percentage of 174. Gallic acid 7.72 mg, Rutin 29.25 mg, Quercetin 27.6 mg, kaempferol 34.42 mg, Apigenin 5.25 mg and Catechin 25.8 mg/100 gm of Castanea fruit was obtained. The inhibitory effect of polyphenol and saponin extracts on LOX activity was at 100 and 40 µg respectively. Line weaver-Burk plot was used to investigate the type of inhibition that was found non-competitive. However, these extracts were studied at concentrations of 500, 250 and 125 µg on bacteria resistance. Polyphenols had the best effect at 500 and 125 µg on E. coli and Pseudomonas bacteria respectively. Whereas, saponin had the best effect at 250 and 125 µg for E. coli and 125 µg for Pseudomonas.

show abstract

Section: Inhibition Type Of Lox Activitymentioning

confidence: 99%

Evaluation of the effect of Castanea sativa extracts on lipoxygenase activity

2022

View full text Add to dashboard Cite

show abstract

“…The interaction between natural polyphenols and the enzyme is of high importance, given the fact that it is considered the potential target through which the biological action of such compounds manifests itself. Consequently, LOX inhibitors have been studied as potential therapeutic agents for the treatment of inflammatory and allergic disorders [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Compounds that act upon the enzyme through this mechanism have a reduction potential, releasing electrons and protons [ 46 , 47 ]. Polyphenols and flavonoids found in plant extracts and fractions have inhibitory activity over the enzyme through the aforementioned mechanism, which is a well-known fact in the scientific literature [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of Tagetes erecta L. Flowers

et al. 2021

View full text Add to dashboard Cite

Tagetes erecta L. is a popular ornamental plant of the Asteraceae family, which is widely cultivated not only for its decorative use, but also for the extraction of lutein. Besides carotenoid representatives, which have been extensively studied, other important classes of secondary metabolites present in the plant, such as polyphenols, could exhibit important biological activities. The phytochemical analysis of a methanolic extract obtained from T. erecta inflorescences was achieved using liquid chromatography–mass spectrometry (LC-MS) techniques. The extract was further subjected to a multistep purification process, which allowed the separation of different fractions. The total extract and its fractions contain several polyphenolic compounds, such as hydroxybenzoic and hydroxycinnamic acid derivatives, flavonols (especially quercetagetin glycosides), and several aglycons (e.g., quercetin, patuletin). One of the fractions, containing mostly quercetagitrin, was subjected to two different antioxidant assays (metal chelating activity and lipoxygenase inhibition) and to in vitro cytotoxicity assessment. Generally, the biological assays showed promising results for the investigated fraction compared to the initial extract. Given the encouraging outcome of the in vitro assays, further purification and structural analysis of compounds from T. erecta extracts, as well as further in vivo investigations are justified.

show abstract

“…Extracts from different plants are diverse in polyphenol contents and differ in their profiles and effectiveness in terms of inactivating lipoxygenase activity (Loncaric et al 2021;Ratnasari, Walters, and Tsopmo 2017). Zhang et al (2019) found that chlorogenic acid could bind to soybean lipoxygenase with the highest affinity, followed by caffeic acid, ferulic acid, naringenin, and chrysin. Furthermore, the binding was related to enzyme inhibition.…”

Section: Polyphenols As Reactive Oxygen Species-and Free Radical-scav...mentioning

confidence: 99%

Inhibitory mechanisms of polyphenols on heme protein-mediated lipid oxidation in muscle food: New insights and advances

Bak

Goran

et al. 2022

Critical Reviews in Food Science and Nutrition

View full text Add to dashboard Cite

Lipid oxidation is a major cause of quality deterioration that decreases the shelf-life of muscle-based foods (red meat, poultry, and fish), in which heme proteins, particularly hemoglobin and myoglobin, are the primary pro-oxidants. Due to increasing consumer concerns over synthetic chemicals, extensive research has been carried out on natural antioxidants, especially plant polyphenols. The conventional opinion suggests that polyphenols inhibit lipid oxidation of muscle foods primarily owing to their strong hydrogen-donating and transition metal-chelating activities. Recent developments in analytical techniques (e.g., protein crystallography, nuclear magnetic resonance spectroscopy, fluorescence anisotropy, and molecular docking simulation) allow deeper understanding of the molecular interaction of polyphenols with heme proteins, phospholipid membrane, reactive oxygen species, and reactive carbonyl species; hence, novel hypotheses regarding their antioxidant mechanisms have been formulated. in this review, we summarize five direct and three indirect pathways by which polyphenols inhibit heme protein-mediated lipid oxidation in muscle foods. we also discuss the relation between chemical structures and functions of polyphenols as antioxidants.

show abstract

Evaluation of inhibitory activity of natural plant polyphenols on Soybean lipoxygenase by UFLC-mass spectrometry

Cited by 20 publications

References 39 publications

Evaluation of the effect of Castanea sativa extracts on lipoxygenase activity

Evaluation of the effect of Castanea sativa extracts on lipoxygenase activity

Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of Tagetes erecta L. Flowers

Inhibitory mechanisms of polyphenols on heme protein-mediated lipid oxidation in muscle food: New insights and advances

Contact Info

Product

Resources

About