Evaluation of insulated isothermal PCR devices for the detection of avian influenza virus

Lee, Jiho; Cho, Andrew Y.; Ko, Hsiao-Han; Ping, Jia-Fong; Ma, Lijuan; Chai, Chuan-Fu; Noh, Jin-Yong; Jeong, Jei-Hyun; Jeong, Sol; Kim, Yujin; Kim, Junbeom; Kim, Kyu-Jik; Lee, Sun-Hak; Lee, Joong‐Bok; Park, Seung‐Yong; Choi, In‐Soo; Song, Chang‐Seon

doi:10.1016/j.jviromet.2021.114126

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…Recently, insulated isothermal PCR (iiPCR) devices were developed and assessed for the detection of avian influenza. The field-deployable POCKIT Micro DUO Nucleic Acid Analyzer needs 45 min, while the POCKIT Central Nucleic Acid Analyser processes results in 85 min to perform an iiPCR analysis [ 32 ]. In terms of time to result, both devices are still not as fast as a probe-based RPA assay under field conditions.…”

Section: Discussionmentioning

confidence: 99%

Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Avian Influenza Virus H9N2 HA Gene

Yehia¹,

Eldemery

Arafa³

et al. 2021

Veterinary Sciences

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The H9N2 subtype of avian influenza A virus (aIAV) is circulating among birds worldwide, leading to severe economic losses. H9N2 cocirculation with other highly pathogenic aIAVs has the potential to contribute to the rise of new strains with pandemic potential. Therefore, rapid detection of H9 aIAVs infection is crucial to control virus spread. A qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of aIAV subtype H9N2 was developed. All results were compared to the gold standard (real-time reverse transcription polymerase chain reaction (RT-PCR)). The RT-RPA assay was designed to detect the hemagglutinin (HA) gene of H9N2 by testing three pairs of primers and a probe. A serial concentration between 106 and 100 EID50 (50% embryo infective dose)/mL was applied to calculate the analytical sensitivity. The H9 RT-RPA assay was highly sensitive as the lowest concentration point of a standard range at one EID50/mL was detected after 5 to 8 min. The H9N2 RT-RPA assay was highly specific as nucleic acid extracted from H9 negative samples and from other avian pathogens were not cross detected. The diagnostic sensitivity when testing clinical samples was 100% for RT-RPA and RT-PCR. In conclusion, H9N2 RT-RPA is a rapid sensitive and specific assay that easily operable in a portable device for field diagnosis of aIAV H9N2.

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Section: Discussionmentioning

confidence: 99%

Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Avian Influenza Virus H9N2 HA Gene

Yehia¹,

Eldemery

Arafa³

et al. 2021

Veterinary Sciences

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“…The majority of modern approaches rely on PCR (conventional PCR, RT-PCR, etc.) [96] , [97] , [98] , [99] or immunoassay technologies [100] . PCR technologies have more sensitivity and specificity than other molecular procedures, and they take less time [101] .…”

Section: Influenza Detectionmentioning

confidence: 99%

Recent Advances in Biosensors for Detection of COVID-19 and Other Viruses

Patel

Surve

Parmar

et al. 2023

IEEE Rev. Biomed. Eng.

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This century has introduced very deadly, dangerous, and infectious diseases to humankind such as the influenza virus, Ebola virus, Zika virus, and the most infectious SARS-CoV-2 commonly known as COVID-19 and have caused epidemics and pandemics across the globe. For some of these diseases, proper medications, and vaccinations are missing and the early detection of these viruses will be critical to saving the patients. And even the vaccines are available for COVID-19, the new variants of COVID-19 such as Delta, and Omicron are spreading at large. The available virus detection techniques take a long time, are costly, and complex and some of them generates false negative or false positive that might cost patients their lives. The biosensor technique is one of the best qualified to address this difficult challenge. In this systematic review, we have summarized recent advancements in biosensor-based detection of these pandemic viruses including COVID-19. Biosensors are emerging as efficient and economical analytical diagnostic instruments for early-stage illness detection. They are highly suitable for applications related to

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“…In addition, this system exhibited desirable specificity with a low detection limit of ∼12 IU/mL. Seasonal influenza is a highly infectious and fast-spreading respiratory disease. − Efforts have been made in developing accurate and rapid POC influenza diagnostic tests. − Microchips have attracted tremendous attention because this system enabled the integration of multiple laboratory processes into simple platforms with high reaction efficiency. A polycarbonate (PC)–polydimethylsiloxane (PDMS) hybrid microchip with an epoxy silica sol–gel coating was fabricated to detect influenza virus with europium NPs (EuNPs) .…”

Section: Microscale Systems “See” In Virusesmentioning

confidence: 99%