Evaluation of insulin-like growth factor-I gene polymorphism on growth traits and yearling fleece weight in goats

Kurdistani, Zana Karimi; Rostamzadeh, Jalal; Rashidi, Amir; Davis, Michael

doi:10.1016/j.smallrumres.2012.08.003

Cited by 10 publications

(11 citation statements)

References 19 publications

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“…The PCR-SSCP analysis of the 5' flanking region of the goat IGF-1 gene in two breeds of goats reared in Iran revealed a novel SNP variation, which was associated with growth traits and yearling fleece weight (Kurdistani et al, 2013). Our study confirmed a high degree of genetic variability in GHR and IGF-1 genes in both cattle and buffalo.…”

Section: Discussionsupporting

confidence: 69%

Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo

Ramesha¹,

Rao²,

Basavaraju³

et al. 2016

SA J. An. Sci.

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The identification of genetic polymorphisms in the genes that play a crucial role in regulatiing growth and development of livestock enables us to evaluate the biological similarities and to acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic variability in the bovine growth hormone receptor (GHR), insulin-like growth factor 1 (IGF-1) and insulin-like growth factor binding protein 3 (IGFBP-3) genes among Bos indicus (Malnad Gidda, Khillar), Bos taurus (Holstein Friesian, Jersey) cattle and Asian water buffalo Bubalus bubalis (Murrah, Surti) using polymerase chain reactionsingle strand conformation polymorphism (PCR-SSCP) analysis. These polymorphisms were confirmed by direct sequencing. The comparative gene sequence analysis in cattle and buffalo breeds revealed 18 single nucleotide polymorphisms (SNPs) across different loci. Eight SNPs were detected in the bovine growth hormone receptor (GHR) gene, of which four were found in the promoter region and four in the exon 4 region. In the IGF-1 gene, two SNPs were observed in the 5ˈUTR, three SNPs in the intron 3 region and two SNPs in the coding region of exon 4. Three SNPs were detected in the exon 2 region of the bovine IGFBP-3 gene. The frequency of rare alleles observed in the present study ranged from 0.04 to 0.16. The present results revealed high levels of genetic variability in the GHR, IGF-1 and IGFBP-3 genes in cattle and buffalo reared in India.

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Section: Discussionsupporting

confidence: 69%

Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo

Ramesha¹,

Rao²,

Basavaraju³

et al. 2016

SA J. An. Sci.

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“…Significant association of the polymorphism in IGF-1 gene was observed with daily gain in Baluchi sheep (Tahmoorespur et al, 2009). Likewise, same polymorphism was associated with growth traits in sheep and in Kurdish goat (Kazemi et al, 2011;Kurdistani et al, 2013). In contrast to our results, Wang et al, (2011) studied the same polymorphism in three breeds of goats and found no significant association with birth weight.…”

Section: Growth Hormone Gene (Gh)contrasting

confidence: 75%

Polymorphisms in Growth Hormone (Gh) and Insulin-Like Growth Factor-1 (Igf-1) Gene and Their Association With Growth Traits in Beetal Goat

Shareef¹

2018

PAKJAS

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Beetal is one of the most famous goat breed of Pakistan which is also present in parts of Indian Punjab. It is popular for its larger body size, higher body weight, and good ability to produce milk. In current study, polymorphism in Growth Hormone (GH) and Insulin-like Growth Factor-1 (IGF-1) genes and their association with growth-related traits were studied in Beetal goat. On the basis of previously reported mutations, a set of 7 primer pairs were designed to screen PCR-RFLP based polymorphisms in 60 random selected Beetal goats varying in body weight. The IGF-1 gene was found polymorphic and following three genotypes (AA, AB, and BB) were detected in tested animals with the frequency of mutant (B) and wild-type (A) alleles as 0.35 and 0.65 respectively. Homozygous carrier genotype (BB) was significantly associated (P<0.05) with higher body weight (47.13±0.78kg) followed by heterozygous carrier (BB) and wild-type (AA) genotype associated with 40.45±0.31kg and 38.18±0.37kg body weight respectively. Additionally, maximum wither height and body length were observed in BB (32.25 ± 0.33, 31.93±0.27 inches) genotype followed by AB (31.93±0.27, 31.16±0.35) and AA (31.62±0.68, 30.87±0.86) genotype. Moreover, maximum heart girth was also observed in BB (33.65±0.11) genotype. All known point mutations of GH gene were found monomorphic in the tested animals. Upon sequencing of GH gene, one non-synonymous SNP was identified in exon 2 at 825bp position that caused a change of amino acid Threonine into Alanine. And likewise one insertion variant in intron 4 was observed at position 1546bp in this gene. The results showed that homozygous carrier animals, having BB genotype in IGF-1 gene, were having the highest values for growth traits suggesting the additive effects of mutant (B) allele on growth traits in this breed. On the basis of our results it is suggested that the identified markers can be used in marker-assisted-selection (MAS) in Beetal goat.

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“…PCR-SSCP analysis of goat IGF-I gene was used to detect the polymorphisms in two Iranian goat breeds (Karimi Kurdistani et al, 2013) which revealed novel G to A transition (g.1617 G >A). Same authors used PCR-RFLP analysis of a part of intron and exon 4 of goat IGF-I gene to identify the associations between IGF-I /HaeIII polymorphism and growth trait.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of insulin-like growth factor-I gene polymorphism in Egyptian small ruminant breeds

Othman¹,

Abdel-Samad²,

El-Maaty³

2016

Afr. J. Biotechnol.

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Evaluation of insulin-like growth factor-I gene polymorphism on growth traits and yearling fleece weight in goats

Cited by 10 publications

References 19 publications

Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo

Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo

Polymorphisms in Growth Hormone (Gh) and Insulin-Like Growth Factor-1 (Igf-1) Gene and Their Association With Growth Traits in Beetal Goat

Evaluation of insulin-like growth factor-I gene polymorphism in Egyptian small ruminant breeds

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