Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays

Muthusamy, Sasikumar

doi:10.14264/uql.2016.968

Cited by 1 publication

(2 citation statements)

References 252 publications

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“…Subsequently, investigating PAH uptake (surrogate bioavailability) and metabolism in human liver cells (surrogate liver). The results from my PhD complements the bioaccessibility and uptake of metal/loids data (Xia, 2016 -PhD thesis) as well as toxicity of mixtures of PAHs and metal/loids (Muthusamy, 2016 PhD thesis), both aspects of the larger project aimed towards better understanding and refining risk assessment of these chemical mixtures.…”

Section: Project Backgroundmentioning

confidence: 87%

“…Nap, Phe, Pyr and B[a]P) before being spiked onto soils at 10 mg and 1000 mg Kg -1 soil spikes for Nap, Phe and Pyr were chosen to reflect the high end concentrations found in Australian gasworks sites (Thavamani et al, 2011). This is also based in toxicological data suggesting no cytotoxic effects were observed on HepG2 cells exposed to Nap, Phe and Pyr at their maximum soluble concentration (Muthusamy, 2016 PhD thesis). Similar concentrations up to 1000 mg Kg -1 have been used for Phe and Pyr in recent studies to refine estimates of human exposure to PAHs via incidental soil ingestion (Juhasz et al, 2016).…”

Section: Spiking Of Soil Samplesmentioning

confidence: 99%

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Health risk assessment of mixed contaminants: Interaction of metals on the uptake of polycyclic aromatic hydrocarbons in human liver cells

Lal¹

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A key area of uncertainty in human health risk assessment of chemical mixtures is the application of additivity of dose or effects, which may overestimate or underestimate the actual risk. In this PhD thesis, interaction effects among mixtures of benzo [a]pyrene (B[a]P), pyrene (Pyr), phenanthrene (Phe) and naphthalene (Nap) in the presence or absence of arsenic (As), cadmium (Cd), lead (Pb) is elucidated for bioaccessibility, uptake (surrogate bioavailability) and metabolism of PAHs in human systems using in vitro models. A simulated human digestion based on the use of an in vitro model (Unified BARGE Method) was used to quantify PAH bioaccessibility from exposure to contaminated soils, whereas a human liver hepatocellular (HepG2) cell line (surrogate liver) was used as an in vitro model to quantify uptake and metabolism of PAHs. This PhD To investigate bioaccessibility of PAHs, seven chemically-variant top soils were collected from background sites in Australia and were spiked with PAHs and aged up to 90 days.The UBM results suggest that the most significant interactions between PAHs and soil components controlling its bioaccessibility was completed by the first 7 days of ageing condition of this study. It is noted that there was a significant lower bioaccessibility of Data from pure solution study suggests that uptake of PAHs and in the presence of As, Cd, and Pb in HepG2 cells generally showed less than additive effects among binary, ternary and seven compound mixtures of B[a]P, Pyr, Phe and Nap. Notably, the % uptake of Phe in HepG2 cells was found to decrease significantly in the seven compound mixture (p < 0.05) which had As, Cd and Pb. Using UBM-extracted solutions, interaction effects on uptake of PAHs in HepG2 cells was found to be both additive and less than additive.Moreover, the % uptake data from UBM-extracted real soil samples showed a decrease in % uptake of B[a]P, Pyr, Phe and Nap by 0.7 %, 1.8 %, 0.4 % and 0.04 %, respectively when compared to their individual PAH % uptake in HepG2 cells from exposure to UBMextracted spiked soil samples.Results of bioaccessibility, uptake and metabolism of B[a]P, Pyr, Phe and Nap as individual compounds or mixtures in the presence or absence of As, Cd and Pb provide significant new understanding towards interaction effects of these compounds in the context of human health risk assessment. For assessment of soils contaminated with PAHs and metal/loids, it clarifies situations where additive effects can be expected rather than assuming additivity as a general rule in risk assessment of chemical mixtures.4

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Section: Project Backgroundmentioning

confidence: 87%

Section: Spiking Of Soil Samplesmentioning

confidence: 99%

Health risk assessment of mixed contaminants: Interaction of metals on the uptake of polycyclic aromatic hydrocarbons in human liver cells

Lal¹

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show abstract

Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays

Cited by 1 publication

References 252 publications

Health risk assessment of mixed contaminants: Interaction of metals on the uptake of polycyclic aromatic hydrocarbons in human liver cells

Health risk assessment of mixed contaminants: Interaction of metals on the uptake of polycyclic aromatic hydrocarbons in human liver cells

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