Evaluation of interleukin-6 and soluble receptors of tumor necrosis factor for early diagnosis of neonatal infection

Messer, J; Eyer, Didier; Donato, Lionel; Gallati, H.; Matis, Jacqueline; Simeoni, Umberto

doi:10.1016/s0022-3476(96)70123-3

Cited by 183 publications

(121 citation statements)

References 16 publications

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“…Control children were sampled for baseline CRP levels measurement. Similar categorization was done in India by Bhartiya et al (2000) and in France by Messer et al (1996). In France, Messer et al (1996) found 15.49% cases of proven septicemia, 49.29% probable septicemia and 35.21% cases were non-septicemic febrile patient.…”

Section: Resultssupporting

confidence: 66%

Isolation and identification of Bacteria and their drug susceptibility pattern in children to establish the correlation between childhood Septicemia with C-reactive protein (CRP) levels

et al. 2015

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Context: Neonatal sepsis is one of the most important causes of morbidity and mortality and C-reactive protein (CRP) an excellent biomarker has significant diagnostic and prognostic value for the treatment of septicemia patient. Objectives: To isolate and identify viable pathogens from blood culture and their antibiogram and to correlate CRP levels with septicemic child. Materials and Methods: A total of 273 cases among which 233 were clinically suspected septicemia cases and 40 were healthy controls in age group 0 day to 15 years were selected from United Hospital Ltd., Dhaka Bangladesh. Blood culture was analyzed by the instrument BACTEC 9120 series. CRP was measured from blood serum by the auto biochemical analyzer OLYMPUS AU 640 followed by immuno-turbidimetric method. The organisms were isolated by inoculation on blood agar and MacConkey agar media. Identification of the organisms was done by colony morphology, gram staining and biochemical tests. Sensitivity of isolates was done against antimicrobial agents by disc diffusing method. Results: Blood samples in total 233 cases of suspected septicemia in children were studied between the ages of 0 day to 15 years. Culture proven septicemia 39 (16.74%), probable septicemia 136 (58.37%) and nonsepticemic febrile patients 58 (24.90%) were found. The highest rate of blood culture positivity found among 5-10 yrs. age group (25.64%). Salmonella typhi (41.03%) was the most common infective agent. The rate of blood culture positivity was significantly higher (p<0.001) among patients without antimicrobial therapy (23.74%) than those in patients with antimicrobial therapy (6.38%). S. typhi were 75% sensitive to Ceftriaxone while azithromycin showed high rate resistance (85.71%). E. coli and Klebsiella pneumoniae were highly sensitive (100%) to imipenem but E. coli resistant (100%) to amikacin, amoxyclavonic acid ciprofloxacin, gentamicin, Cefepime and netilmicin. Resistant (100%) to gentamicin was observed from K. pneumoniae. Mean CRP values (mg/l) of blood culture proven septicemia group, probable septicemia group, non-septicemic febrile group and control group were 70.42, 34.05, 3.08 and 0.98 respectively. Both proven septicemia and suspected septicemia cases showed CRP concentration above the cut-off value (>6 mg/l) and p value significant (p<0.001). Statistically significant difference (p<0.001) was found when mean CRP level of proven septicemia group, probable septicemia group and non-septicemic febrile group each compared with control group. CRP concentration were significantly (p < 0.001) different among three study group. Conclusion: Most of the gram negative bacteria isolated from blood culture showed resistance to commonly used antibiotics. The predominant infective isolate was Salmonella typhi. In this study, CRP level is high (cut-off value 6 mg/l) both in proven and probable septicemia group. CRP may have a good biomarker tools in diagnostic and prognostic value. Investigation of blood culture should be done before antimicrobial therapy,

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Section: Resultssupporting

confidence: 66%

Isolation and identification of Bacteria and their drug susceptibility pattern in children to establish the correlation between childhood Septicemia with C-reactive protein (CRP) levels

et al. 2015

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“…CRP specificity varied in initial determination from 92% to 96% and decreased to 76 -86% for the third measurement (35)(36)(37)(38). IL-6 in umbilical cord blood has been reported to be consistently increased in newborn infants who develop sepsis within 48 h of birth, with specificities ranging from 88% to 93% (39 ).…”

Section: Discussionmentioning

confidence: 99%

C-Reactive Protein, Interleukin-6, and Procalcitonin in the Immediate Postnatal Period: Influence of Illness Severity, Risk Status, Antenatal and Perinatal Complications, and Infection

Chiesa

Pellegrini²,

Panero³

et al. 2003

Clinical Chemistry

225

167

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Background: Studies of the diagnostic accuracy of most laboratory tests for early-onset neonatal sepsis have yielded variable results. We investigated whether some of this variation might be attributable to differences in population baseline severity and risk status as well as to specific ante-and perinatal variables, independent of the presence of neonatal infection. Methods: The Score for Neonatal Acute Physiology (SNAP) was used to define illness severity, with SNAP Perinatal Extension (SNAP-PE) used to define the combined physiologic and perinatal mortality risk. A total of 134 ill newborns (19 with early-onset infection and 115 with no infection) were available for simultaneous analysis of the association of SNAP, SNAP-PE, and maternal and perinatal variables with C-reactive protein (CRP), interleukin-6 (IL-6), and procalcitonin (PCT) concentrations at birth and at 24 and 48 h of life. Results: Early-onset neonatal infection was associated with significant increases in CRP, IL-6, and PCT concentrations at all three time points, independent of illness severity. However, among babies without infection, higher SNAP and SNAP-PE scores were associated with higher IL-6 concentrations at birth. Certain maternal or perinatal variables altered IL-6 and PCT values in the infected as well as in the uninfected neonates. However, if different cutoff points were used at any of

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“…15,16 Since, however, IL-8 plasma half-life comprises only 1-3 h, its determination may lead to false-negative results when sampling is performed later in the course of disease. 3,4,28 Our hopes to predict EOBI and to close the diagnostic gap with LBP did not hold true, since its diagnostic value was remarkably lower than that of IL-8 in plasma (Table 2) or lysed whole blood. 4 In conclusion, LBP is not sufficiently sensitive to predict EOBI.…”

Section: Lbp Concentrations In Noninfected Neonatesmentioning

confidence: 94%

Lipopolysaccharide-binding protein in noninfected neonates and those with suspected early-onset bacterial infection

et al. 2006

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Objectives: To investigate postnatal lipopolysaccharide-binding protein (LBP) kinetics in term neonates and to test its diagnostic accuracy for earlyonset bacterial infection (EOBI).Study design: A total of 99 neonates with clinical and serological signs of EOBI comprised the study group; 198 neonates with risk factors, but without EOBI, served as controls. LBP, C-reactive protein (CRP) and interleukin-8 (IL-8) were determined.Results: LBP in the noninfected group increased until 24 h after birth (P<0.05 vs 6 h). LBP and CRP correlated strongly in neonates with suspected EOBI (r ¼ 0.63). Although LBP reached a higher sensitivity than CRP 6 and 12 h after clinical suspicion (45 (24-68) and 79% (54-94) vs 9 (0-24) and 39% (17-64); P<0.05)), EOBI was most reliably detected by IL-8. Conclusion: LBP kinetics were age-dependent. LBP was not sufficiently sensitive in the prediction of EOBI.

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Evaluation of interleukin-6 and soluble receptors of tumor necrosis factor for early diagnosis of neonatal infection

Cited by 183 publications

References 16 publications

Isolation and identification of Bacteria and their drug susceptibility pattern in children to establish the correlation between childhood Septicemia with C-reactive protein (CRP) levels

Isolation and identification of Bacteria and their drug susceptibility pattern in children to establish the correlation between childhood Septicemia with C-reactive protein (CRP) levels

C-Reactive Protein, Interleukin-6, and Procalcitonin in the Immediate Postnatal Period: Influence of Illness Severity, Risk Status, Antenatal and Perinatal Complications, and Infection

Lipopolysaccharide-binding protein in noninfected neonates and those with suspected early-onset bacterial infection

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