Evaluation of larval culture and conventional PCR methods for the detection of Strongylus vulgaris in equines of Iran

Alborzi, Alireza; Larki, Sara; Zeinali, Abbas

doi:10.3906/vet-2001-88

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…The high sensitivity of PCR ended up being fundamental for diagnosing S. vulgaris , considering that this nematode was even detected in fecal samples that had been considered negative (based on the EPG count) or presented low counts. Similar findings were reported in diagnosing S. vulgaris in equids in Denmark and Iran ( Bracken et al, 2012 ; Alborzi et al, 2020 ). S. vulgaris is considered to be the intestinal nematode species that are highly pathogenic for equids, given the occurrence of thromboses and aneurysms in the mesenteric arteries, due to the migration of its larval forms ( Carvalho, 2006 ).…”

Section: Discussionsupporting

confidence: 87%

“…Almost all the fecal samples from the ponies in this study (96.3%) were found to be positive for S. vulgaris . Lower positivity rates than in the present study, were also obtained through PCR from horses in Denmark (12.1%), and Germany (1.9%), and among horses and donkeys in Iran (8.8% and 9.7%, respectively) ( Bracken et al, 2012 ; Kaspar et al, 2016 ; Alborzi et al, 2020 ). The presence of DNA from this parasite in the feces of different equids that were kept in different countries emphasizes the widespread distribution of this nematode.…”

Section: Discussioncontrasting

confidence: 64%

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First microscopic and molecular parasitological survey of Strongylus vulgaris in Brazilian ponies

Martins

Coelho

Corrêa

et al. 2023

Rev. Bras. Parasitol. Vet.

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The frequency of gastrointestinal parasites with an emphasis on Strongylus vulgaris was investigated among the Brazilian Pony breed kept on farms in the municipality of Teresópolis, state of Rio de Janeiro. Fecal samples were collected in three stud farms: A (n= 22 animals), B (n= 3), and C (n= 2). Fecal samples were subjected to the quantitative Mini-FLOTAC technique, using three different solutions, and to qualitative techniques. The parasite prevalence was found to be 81.4%. Eggs from strongylids were identified in 74% of the ponies. Eggs of Parascaris spp. were detected in 22.7% of the animals, which were all females of farm A. At this locality, mares were kept with their foals in fenced paddocks all the time. The NaCl solution of d = 1.200 g/ml was generally the one that presented the highest frequency of diagnosis of nematode eggs and the highest mean of fecal eggs per gram. The fecal samples were also subjected to the polymerase chain reaction for amplification of DNA from the ITS2 region for Strongylus vulgaris. Twelve samples presented nucleotide sequences for S. vulgaris. In the end, this study revealed the high frequency (96.3%) of S. vulgaris among ponies on farms in Teresópolis, Rio de Janeiro, Brazil.

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Section: Discussionsupporting

confidence: 87%