Evaluation of lateral flow immunochromatographic assay for diagnostic accuracy of cryptococcosis

Abstract

Background: Cryptococcus is a conditional pathogenic fungus causing cryptococcosis, which is one of the most serious fungal diseases faced by humans. Lateral flow immunochromatographic assay (LFA) is successfully applied to the rapid detection of cryptococcal antigens. Methods: Studies were retrieved systematically from the Embase, PubMed, Web of Science, and Cochrane Library before July 2019. The quality of the studies was assessed by Review Manager 5.0 based on the Quality Assessment of Diagnostic Accuracy Study guidelines. The extracted data from the included studies were analyzed by Meta-DiSc 1.4. Stata 12.0 software was used to detect the publication bias.Results: A total of 15 articles with 31 fourfold tables were adopted by inclusion and exclusion criteria. The merged sensitivity and specificity in serum were 0.98 and 0.98, respectively, and those in the cerebrospinal fluid were 0.99 and 0.99, respectively.Conclusions: Compared to the urine and other samples, LFA in serum and cerebrospinal fluid is favorable evidence for the diagnosis of cryptococcosis with high specificity and sensitivity.

Serology anno 2021—fungal infections: from invasive to chronic

“…Simultaneous detection of anti-mannan antibodies (AMA) improved sensitivity and specificity (83% and 86%) [45], respectively. Several test kits for the detection of mannan antigen (MA) of C. albicans and AMA show variable performance [46][47][48], see [55][56][57][58], which avoid serum pretreatment and support urinary cryptococcal antigen testing, which is useful for screening [59,60]. The LFA is a rapid procedure, there are various test kits on the market, see Table 5;…”