Evaluation of latex agglutination tests for fibrin–fibrinogen degradation products in the forensic identification of menstrual blood

Akutsu, Tomoko; Watanabe, Ken; Motani, Hisako; Iwase, Hirotaro; Sakurada, Koichi

doi:10.1016/j.legalmed.2011.10.003

Cited by 11 publications

(4 citation statements)

References 15 publications

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“…Accordingly, it is actively used in criminal investigations. Clarification of whether the sample concerns menstrual blood [13][14][15][16][17], maternal blood [18][19][20], fetal blood [21,22], or postmortem blood [15,23] is also often required, and this identification is performed through assays that target proteins or expressed genes that are characteristic of each blood type.…”

Section: Flow Of Forensic Examinations Using Biological Samplesmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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Although, DNA typing plays a decisive role in the identification of persons from blood and body fluid stains in criminal investigations, clarifying the origin of extracted DNA has also been considered an essential task in proving a criminal act. This review introduces the importance of developing precise methods for body fluid identification. Body fluid identification has long relied on enzymatic methods as a presumptive assay and histological or serological methods as a confirmatory assay. However, because the latest DNA typing methods can rapidly obtain results from very small and even old, poorly preserved samples, the development of a novel corresponding body fluid identification method is required. In particular, an immunochromatographic method has been introduced to identify saliva and semen from sexual crimes. In addition, for vaginal fluid identification, attempts have been made in the past decade to introduce a method relying on body fluid-specific mRNA expression levels. At present, the development of molecular biological methods involving microRNA, DNA methylation, and resident bacterial DNA is ongoing. Therefore, in criminal investigations, body fluid identification is an essential task for correctly applying the results of DNA typing, although further research and development are required.

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Section: Flow Of Forensic Examinations Using Biological Samplesmentioning

confidence: 99%

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

2020

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“…Recently two screening tests for the identification of menstrual blood were described, that utilize antibodies for fibrinfibrinogen degradation products (latex agglutination test, D-dimer assay) [2,3]. Neither test has been confirmed and validated for forensic use.…”

Section: Introductionmentioning

confidence: 99%

RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise

Haas

Hanson

Anjos

et al. 2014

Forensic Science International: Genetics

102

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The European DNA Profiling Group (EDNAP) organized a fourth and fifth collaborative exercise on RNA/ DNA co-analysis for body fluid identification and STR profiling. The task was to identify dried menstrual blood and vaginal secretion stains using specific RNA biomarkers, and additionally test 3 housekeeping genes for their suitability as reference genes. Six menstrual blood and six vaginal secretion stains, two dilution series (1/4-1/64 pieces of a menstrual blood/vaginal swab) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 24 participating laboratories, using RNA extraction or RNA/DNA co-extraction methods. Two novel menstrual blood mRNA multiplexes were used: MMP triplex (MMP7, MMP10, MMP11) and MB triplex (MSX1, LEFTY2, SFRP4) in conjunction with a housekeeping gene triplex (B2M, UBC, UCE). Two novel mRNA multiplexes and a HBD1 singleplex were

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“…The analysis of body fluid-specific mRNA expression levels has recently been reported as a promising tool for the identification of body fluids in biological stains (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). In 2005, Juusola and Ballantyne reported multiplex mRNA profiling for the identification of body fluids using agarose gel electrophoresis and capillary electrophoresis format (8).…”

mentioning

confidence: 99%

Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography^,^,

Matsumura

Matsusue

Waters

et al. 2016

Journal of Forensic Sciences

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Forensic laboratories are often faced with cases in which methamphetamine hydrochloride-mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. The application of mRNA expression analysis to samples that showed a false-negative with immunochromatography was investigated as an alternative approach that did not depend on the antigen-antibody reaction. Real-time PCR was used to examine the expression levels of blood markers such as glycophorin A, spectrin beta, and hemoglobin beta. Hemoglobin beta was the only marker that was specifically detected in blood, while glycophorin A was useful for determining human specificity. Hemoglobin beta showed good detection sensitivity and was detectable in 37-year-old blood stains. Hemoglobin beta was exclusively detectable in methamphetamine hydrochloride-mixed blood stains. Detergents and disinfectants did not significantly influence mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography.

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Evaluation of latex agglutination tests for fibrin–fibrinogen degradation products in the forensic identification of menstrual blood

Cited by 11 publications

References 15 publications

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise

Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography^,^,

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Evaluation of latex agglutination tests for fibrin–fibrinogen degradation products in the forensic identification of menstrual blood

Cited by 11 publications

References 15 publications

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid

RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise

Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography,,

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Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography^,^,