Evaluation of LC methods for the separation of phenoxymethylpenicillin and its related substances

Zhang, Yongxin; Merken, E.; Porqueras, E.; Roets, Eugène; Hoogmartens, Jos

doi:10.1016/s0928-0987(97)00066-3

Cited by 7 publications

(1 citation statement)

References 7 publications

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“…The selectivity of five isocratic liquid chromatography (LC) methods had been previously examined [1]. It was observed that the method previously selected for the analysis of benzylpenicillin (PG) using a mobile phase of 0.5 M phosphate buffer pH 3.5-water-methanol (10:50:40) also consistently gave good selectivity for phenoxymethylpenicillin (PV) on different stationary phases.…”

Section: Introductionmentioning

confidence: 99%

Interlaboratory study of analysis of phenoxymethylpenicillin by liquid chromatography

Zhang

Roets

Trippen³

et al. 1998

Chromatographia

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SummaryA liquid chromatography method for analysis of phenoxymethylpenicillin has been examined in a collaborative study involving 6 laboratories. The method comprised an isocratic part, which is used in the assay. When the isocratic part is combined with gradient elution, the method is suitable for purity control. Five samples of phenoxymethylpenicillin (potassium salts and acid) were analysed. The main component, the most important side product 4-hydroxyphenoxymethylpenicillin and other impurities were determined. An analysis of variance proved the absence of consistent laboratory bias. Laboratory-sample interaction was not significant. Estimates of the repeatability and reproducibility of the method, expressed as standard deviations (SD) of the result of the determination of phenoxymethylpenicillin, were 0.50 and 0.63 respectively.

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Section: Introductionmentioning

confidence: 99%

Interlaboratory study of analysis of phenoxymethylpenicillin by liquid chromatography

Zhang

Roets

Trippen³

et al. 1998

Chromatographia

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Determination of the purity of phenoxymethylpenicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Čápová

Jobánek

2003

J of Separation Science

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Determination of the purity of phenoxymethylpenicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column A micellar electrokinetic chromatographic and a fast reversed-phase liquid chromatographic method have been developed for determination of the purity of phenoxymethylpenicillin. The optimized running buffer composition was 40 mM phosphateborate -125 mM SDS -3.5% (v/v) methanol. The HPLC method employed a monolithic silica C18 column and a mobile phase composed of phosphate buffer, pH 3.5, and ACN, the flow-rate being 3.5 mL/min. Both methods were successfully validated. Linearity, intermediate precision, limits of quantitation, accuracy, and a good correlation of the HPLC and MEKC results were demonstrated. Both methods proved to be fast and reliable and sufficiently sensitive. A combination of the two methods can be very useful in impurity profiling.

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Synthesis and characterization of potential impurities of Ampicillin trihydrate

Suresh,

Devi,

Aegurla

et al. 2023

Chem. Pap.

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The present research work narratesthe synthesis, origin and characterization of related substances of Ampicillin trihydrate Viz: Ampicillinyl-d-phenylglycine (Ph. Eur impurity E) & l-Ampicillin (Ph. Eur impurity B). The impurity Ampicillinyl-d-phenylglycine generates during the preparation of Ampicillin trihydrate, amidation may occur with carboxylic acid of Ampicillin with d-phenylglycine to give Ampicillinyl-dphenylglycine, the impurity l-Ampicillin may arise due to the presence of traces l-phenylglycine methylester in d-phenylglycine methylester hydrochloride which may result in the formation of l-Ampicllin. A facile synthetic process for these impurities was developed from the commercially available raw materials.

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Evaluation of LC methods for the separation of phenoxymethylpenicillin and its related substances

Cited by 7 publications

References 7 publications

Interlaboratory study of analysis of phenoxymethylpenicillin by liquid chromatography

Interlaboratory study of analysis of phenoxymethylpenicillin by liquid chromatography

Determination of the purity of phenoxymethylpenicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

Synthesis and characterization of potential impurities of Ampicillin trihydrate

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