With the continuous improvement of people's material living standards, public health and national fitness has become a social topic, and has been widely concerned by the public, in terms of the traditional physical education and impact of the concept of health. This not only needs the school to carry on the multi-directional adjustment to the physical education teaching, but also needs the physical education teacher to respond positively to this in order to adapt to the development needs of the times and society. This study uses the analytic hierarchy process (AHP) to construct the professionalization model of physical education teachers in Colleges and Universities, and makes decision on the index weight of the model through fuzzy comprehensive evaluation. The results show that the weight of the first-class indicators of P.E. Teachers' professionalization model from large to small is ability > quality > knowledge, and the consistency ratio (CR) of the model indicators is less than 0.1, and the fuzzy score of the model is 85 points. Therefore, the model of P.E. Teachers' professionalization constructed in this study has consistent indicators and shows good applicability. This does not only provide the basis for the professional development evaluation of College PE teachers, but also provides research ideas for the development of the sports industry from the perspective of public health.