Evaluation of light-harvesting complex proteins as senescence-related protein markers in detached rice leaves

Abstract

Ten light-harvesting complex (Lhc) proteins were investigated to determine which was the most appropriate protein marker of senescence in detached rice leaves. The levels of Lhc proteins were monitored by immunoblot analysis, which was conducted using commercially available antibodies raised against each Lhc protein. Among the Lhc proteins evaluated in this study, Lhca1, Lhcb1, Lhcb2, Lhcb3, and Lhcb5 were not appropriate to be used as senescence markers while others can be used after optimization of the procedure.

Transcriptional Profile Corroborates that bml Mutant Plays likely Role in Premature Leaf Senescence of Rice (Oryza sativa L.)

“…The expression of orthologs of chloroplast precursor-relative and TPR (tetratricopeptide repeat) like super family genes LOC_Os08g09270 , LOC_Os06g02120 and LOC-Os01g25600 were down-regulated in the bml mutant (Table S4). In the bml mutant, the genes of grana degradation, photosynthesis pathway, photosystem I and photosystem II (Photosynthesis - antenna proteins pathway) were shown to be down-regulated, which supports our results by the down-regulation of NYC1 , ATFD3 , ATLFNR2 , LHCA5 and LHCA2 genes [18,51,52] which causes early-leaf senescence. The orthologs of ATCOL2 (a GATA transcription factor) were down-regulated, which can influence leaf senescence by regulating chlorophyll accumulation [35,36].…”

Abscisic acid substantially inhibits senescence of cucumber plants (Cucumis sativus) grown under low nitrogen conditions

“…4, the expression of the LHCP gene and the level of LHCP protein maintained in the plants treated with ABA depended on the concentrations of ABA, while the gene expression and the protein content were decreased by ABA under sufficient-N conditions (Kusneosov et al, 1994;Staneloni et al, 2008;Kang et al, 2009). Structurally unstable LHCII from the degradation of chlorophyll b could be decomposed by protease; subsequently, the thylakoid membrane was degraded in the chloroplasts (Kusaba et al, 2007).…”