Evaluation of ligninolytic activity in spent mushroom substrate from four cultivated mushrooms

Pérez-Chávez, Ana Marión; Alberti, María Melisa; Albertó, Edgardo

doi:10.1016/j.jobab.2022.09.001

Cited by 11 publications

(4 citation statements)

References 37 publications

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“…The specific profile of ligninolytic enzymes produced during solid-state fermentation of white-rot fungi is known to be affected by the substrate used [ 18 , 19 ]. Additionally, laccase activity is commonly reported to exceed the activity of peroxidases in solid-state fermentation on lignocellulosic substrates [ 20 ], as also observed in the present study. It should, however, be pointed out that the presence of other ligninolytic enzymes such as lignin peroxidase was not evaluated in the present study.…”

Section: Resultssupporting

confidence: 83%

Use of sawdust for production of ligninolytic enzymes by white-rot fungi and pharmaceutical removal

Hultberg,

Golovko

2024

Bioprocess Biosyst Eng

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Use of white-rot fungi for enzyme-based bioremediation of wastewater is of high interest. These fungi produce considerable amounts of extracellular ligninolytic enzymes during solid-state fermentation on lignocellulosic materials such as straw and sawdust. We used pure sawdust colonized by Pleurotus ostreatus, Trametes versicolor, and Ganoderma lucidum for extraction of ligninolytic enzymes in aqueous suspension. Crude enzyme suspensions of the three fungi, with laccase activity range 12–43 U/L and manganese peroxidase activity range 5–55 U/L, were evaluated for degradation of 11 selected pharmaceuticals spiked at environmentally relevant concentrations. Sulfamethoxazole was removed significantly in all treatments. The crude enzyme suspension from P. ostreatus achieved degradation of wider range of pharmaceuticals when the enzyme activity was increased. Brief homogenization of the colonized sawdust was also observed to be favorable, resulting in significant reductions after a short exposure of 5 min. The highest reduction was observed for sulfamethoxazole which was reduced by 84% compared to an autoclaved control without enzyme activity and for trimethoprim which was reduced by 60%. The compounds metoprolol, lidocaine, and venlafaxine were reduced by approximately 30% compared to the control. Overall, this study confirmed the potential of low-cost lignocellulosic material as a substrate for production of enzymes from white-rot fungi. However, monitoring over time in bioreactors revealed a rapid decrease in enzymatic ligninolytic activity.

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Section: Resultssupporting

confidence: 83%

Use of sawdust for production of ligninolytic enzymes by white-rot fungi and pharmaceutical removal

Hultberg,

Golovko

2024

Bioprocess Biosyst Eng

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“…In addition, Pleurotus spp. has been studied for its remarkable production of lignocellulolytic enzymes that have a key role in the degradation of complex organic materials [64,65]. Some previous works have revealed that the growing substrate can emphasize the metabolites composition of the fruiting bodies [63].…”

Section: Discussionmentioning

confidence: 99%

Sustainable Management of Anaerobic Digestate: From Biogas Plant to Full-Scale Cultivation of Pleurotus ostreatus

et al. 2023

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In the last decades, the number of studies about the valorization of agricultural by-products has strongly increased due to the growing focus on the circular economy. In this context, mushroom cultivation is raising attention for its nutritional properties and adaptability to different growing conditions. The goal of this project is, therefore, to create a link between two production chains, valorizing the solid anaerobic digestate fraction from the biogas plant as a potential substrate for Pleurotus ostreatus full-scale cultivation. A preliminary in vitro trial shows that the mycelia can colonize at the same rate in both the conventional substrate, which was wheat straw added with sunflower cake and the experimental ones supplemented with corn digestate. The most important and innovative part of the work was full-scale cultivation. Quantitative analyses demonstrate that P. ostreatus is capable of growing on the conventional substrate as well as on the substrate containing 15% of digestate without reducing the mushroom harvest. Even better, digestate seems to stimulate a precocious and more homogeneous production. Chemical and spectroscopic analyses on the fruiting bodies confirm the results; in fact, mushrooms show a comparable structural composition, highlighted with FTIR and SERS spectroscopic evaluations. Moreover, the nutrient content did not change due to the addition of digestate. These promising results demonstrate that anaerobic digestate can become a resource for P. ostreatus cultivation without production losses and maintaining the same qualitative characteristics.

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“…Regarding the first type, after the harvesting period, for each kilogram of mushrooms produced, approximately 5 kg of SMS is generated as a byproduct [156,157]. Improper disposal methods, such as burning or landfilling, can result in water contamination, leading to eutrophication and air pollution.…”

Section: Circular Economy Approach: Waste Reusementioning

confidence: 99%

Pre- and Postharvest Strategies for Pleurotus ostreatus Mushroom in a Circular Economy Approach

Silva,

Ramos,

Lidon

et al. 2024

Foods

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Mushroom cultivation presents a viable solution for utilizing agro-industrial byproducts as substrates for growth. This process enables the transformation of low-economic-value waste into nutritional foods. Enhancing the yield and quality of preharvest edible mushrooms, along with effectively preserving postharvest mushrooms, stands as a significant challenge in advancing the industry. Implementing pre- and postharvest strategies for Pleurotus ostreatus (Jacq.) P. Kumm (oyster mushroom) within a circular economy framework involves optimizing resource use, minimizing waste, and creating a sustainable and environmentally friendly production system. This review aimed to analyze the development and innovation of the different themes and trends by bibliometric analysis with a critical literature review. Furthermore, this review outlines the cultivation techniques for Pleurotus ostreatus, encompassing preharvest steps such as spawn production, substrate preparation, and the entire mushroom growth process, which includes substrate colonization, fruiting, harvesting, and, finally, the postharvest. While novel methodologies are being explored for maintaining quality and extending shelf-life, the evaluation of the environmental impact of the entire mushroom production to identify areas for improvement is needed. By integrating this knowledge, strategies can be developed for a more sustainable and circular approach to Pleurotus ostreatus mushroom cultivation, promoting environmental stewardship and long-term viability in this industry.

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Evaluation of ligninolytic activity in spent mushroom substrate from four cultivated mushrooms

Cited by 11 publications

References 37 publications

Use of sawdust for production of ligninolytic enzymes by white-rot fungi and pharmaceutical removal

Use of sawdust for production of ligninolytic enzymes by white-rot fungi and pharmaceutical removal

Sustainable Management of Anaerobic Digestate: From Biogas Plant to Full-Scale Cultivation of Pleurotus ostreatus

Pre- and Postharvest Strategies for Pleurotus ostreatus Mushroom in a Circular Economy Approach

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