Evaluation of linezolid, vancomycin, gentamicin and ciprofloxacin in a rabbit model of antibiotic-lock technique for Staphylococcus aureus catheter-related infection

Fernández-Hidalgo, Núria; Gavaldá, Joan; Almirante, Benito; Martín, María-Teresa; Onrubia, P. López; Gomis, Xavier; Pahissa, Albert

doi:10.1093/jac/dkp499

Cited by 37 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, data about its efficacy are contradictory, as Lee et al [24] showed that it was not effective over 5 days of ALT in in vitro biofilm models, nor were cefazolin, nafcillin or erythromycin, while Fernandez-Hidalgo et al [25] found that gentamicin ALT was generally effective on S. aureus biofilms in rabbit catheters.…”

Section: Aminoglycosidesmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial lock therapy in central-line associated bloodstream infections: a systematic review

Vassallo

Dunais

Roger³

2015

Infection

View full text Add to dashboard Cite

show abstract

Section: Aminoglycosidesmentioning

confidence: 99%

“…Fernandez-Hidalgo et al [25] showed that linezolid and vancomycin had equivalent activity on MSSA and MRSA strains responsible for catheter-related infections in a rabbit model.…”

Section: Linezolid Lock Therapymentioning

confidence: 99%

Antimicrobial lock therapy in central-line associated bloodstream infections: a systematic review

Vassallo

Dunais

Roger³

2015

Infection

View full text Add to dashboard Cite

show abstract

“…Currently, combination biomaterials and coatings are being developed for the treatment and prevention of biofilm‐related infections. The majority of animal studies that are used to model infections related to these materials primarily involve the use of an initial inoculum of planktonic bacterial cells from batch cultures 1–24. The expectation has been that these planktonic cells would attach to the surface of a biomaterial, medical device, or surrounding tissue and subsequently form a biofilm.…”

Section: The Use Of Planktonic Cells In Animal Modelsmentioning

confidence: 99%

Using biofilms as initial inocula in animal models of biofilm‐related infections

Williams

Costerton

2011

J Biomed Mater Res

View full text Add to dashboard Cite

One of the most practical strategies that has been undertaken to fight biofilm implant-related infections has been the development of coatings on biomaterial devices that can elute antimicrobials into regions of patients' tissues. To date, the majority of animal studies that have been developed to model infections that accompany the use of these materials have primarily involved an initial inoculum of planktonic bacterial cells from batch cultures. Although valuable, data that have been derived from these experiments may not provide important clinical insight into how bacteria in well-established, mature biofilms impact device-related and other clinical infections when they contaminate a patient site or implanted device. In this review, a discussion is presented on the impact that a shift in biofilm research may have if initial inocula of well-established, mature biofilms are used to model biomaterial device-related infections in animal models.

show abstract

“…Moreover several animal models using subcutaneously implanted or vascular catheters were successfully used to evaluate the antimicrobial efficacy of several antibiotics against bacteria such as Staphylococcus aureus and Staphylococcus epidermidis , and to assess the role of regulators or virulence factors in biofilm development [9], [13], [14], [15], [16], [17], [18], [19]. However, these approaches were mainly designed to study short-term biofilm infections, and rarely integrated both microbial and host contribution into development of biofilm infections.…”

Section: Introductionmentioning

confidence: 99%

A Rat Model of Central Venous Catheter to Study Establishment of Long-Term Bacterial Biofilm and Related Acute and Chronic Infections

et al. 2012

View full text Add to dashboard Cite

Formation of resilient biofilms on medical devices colonized by pathogenic microorganisms is a major cause of health-care associated infection. While in vitro biofilm analyses led to promising anti-biofilm approaches, little is known about their translation to in vivo situations and on host contribution to the in vivo dynamics of infections on medical devices. Here we have developed an in vivo model of long-term bacterial biofilm infections in a pediatric totally implantable venous access port (TIVAP) surgically placed in adult rats. Using non-invasive and quantitative bioluminescence, we studied TIVAP contamination by clinically relevant pathogens, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis, and we demonstrated that TIVAP bacterial populations display typical biofilm phenotypes. In our study, we showed that immunocompetent rats were able to control the colonization and clear the bloodstream infection except for up to 30% that suffered systemic infection and death whereas none of the immunosuppressed rats survived the infection. Besides, we mimicked some clinically relevant TIVAP associated complications such as port-pocket infection and hematogenous route of colonization. Finally, by assessing an optimized antibiotic lock therapy, we established that our in vivo model enables to assess innovative therapeutic strategies against bacterial biofilm infections.

show abstract

Evaluation of linezolid, vancomycin, gentamicin and ciprofloxacin in a rabbit model of antibiotic-lock technique for Staphylococcus aureus catheter-related infection

Abstract: Gentamicin showed the highest activity against both MSSA and MRSA biofilms.

Cited by 37 publications

References 19 publications

Antimicrobial lock therapy in central-line associated bloodstream infections: a systematic review

Antimicrobial lock therapy in central-line associated bloodstream infections: a systematic review

Using biofilms as initial inocula in animal models of biofilm‐related infections

A Rat Model of Central Venous Catheter to Study Establishment of Long-Term Bacterial Biofilm and Related Acute and Chronic Infections

Contact Info

Product

Resources

About