Evaluation of Lipases from Wild Microbial Strains as Biocatalysts in Biodiesel Production

Luna, Carlos; Luna, Diego; Bautista, Felipa M.; Calero, Juan; Romero, Antonio A.; Posadillo, Alejandro; Sancho, Enrique; Estévez, Rafael

doi:10.3390/separations5040053

Cited by 6 publications

(3 citation statements)

References 29 publications

(62 reference statements)

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“…The trimyristin (0.75 g, 1 mmol) was directly dissolved in dry ethanol (3 mL), and the mixture was incubated at 308 K for 24 h in the presence of TLIM (0.38 g) as the catalyst. This catalyst was reported for its regioselectivity [21,22]. The mixture was filtered, and the filtrate was diluted with 80% vol ethanol (20 mL).…”

Section: Methodsmentioning

confidence: 99%

Monomyristin and Monopalmitin Derivatives: Synthesis and Evaluation as Potential Antibacterial and Antifungal Agents

et al. 2018

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In the present work, monoacylglycerol derivatives, i.e., 1-monomyristin, 2-monomyristin, and 2-monopalmitin were successfully prepared from commercially available myristic acid and palmitic acid. The 1-monomyristin compound was prepared through a transesterification reaction between ethyl myristate and 1,2-O-isopropylidene glycerol, which was obtained from the protection of glycerol with acetone, then followed by deprotection using Amberlyst-15. On the other hand, 2-monoacylglycerol derivatives were prepared through enzymatic hydrolysis of triglycerides in the presence of Thermomyces lanuginosa lipase enzymes. The synthesized products were analyzed using fourier transform infrared (FTIR) spectrophotometer, gas or liquid chromatography-mass spectrometer (GC-MS or LC-MS), and proton and carbon nuclear magnetic resonance (1H- and 13C-NMR) spectrometers. It was found that monomyristin showed high antibacterial and antifungal activities, while 2-monopalmitin did not show any activity at all. The 1-monomyristin compound showed higher antibacterial activity against Staphylococcus aureus and Aggregatibacter actinomycetemcomitans and also higher antifungal activity against Candida albicans compared to the positive control. Meanwhile, 2-monomyristin showed high antibacterial activity against Escherichia coli. The effect of the acyl position and carbon chains towards antibacterial and antifungal activities was discussed.

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Section: Methodsmentioning

confidence: 99%

Monomyristin and Monopalmitin Derivatives: Synthesis and Evaluation as Potential Antibacterial and Antifungal Agents

et al. 2018

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“…To increase biodiesel performance, eco diesel (ED) is used for their mixture. Eco Diesel is a new biofuel derived from a mixture of monoglycerides (MG) and fatty acid methyl esters (FAME) which can be applied to diesel engines [26][27][28]. ED is a bio additive produced from natural ingredients, consisting of citronella oil, graniol, granile acetate, and citronellal acetate [29].…”

Section: Nomenclaturementioning

confidence: 99%

Engine Performance Using Blended Fuels of Biodiesel and Eco Diesel

Idris¹,

Husin²,

Hermawan³

et al. 2023

Energy Engineering

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Diesel engines is an internal combustion engine with high thermal efficiency, which also uses biodiesel fuel, an environmentally friendly, non-toxic, and low sulfur content. Biodiesel has been around for a long time due to its similar characteristics to diesel fuels which has limited availability. However, several disadvantages are associated with biodiesel, such as poor volatility and high viscosity, which reduces engine performance. Therefore, this study was carried out to improve the diesel engine performance by mixing biodiesel with ecodiesel (ED), an additive produced from natural ingredients that is dissolvable in biodiesel. The biodiesel fuel properties used are density 860 kg/m 3 , dynamic viscosity 4.50E-06 m 2 /s, cetane number 45, and flashpoint 52°C. The results showed that biodiesel-ED mixture could improve engine performance and the optimum performance was at a speed of 3000 rpm on 43.30 (kW), 124.93 (N.m) of the engine torque, and 2.45E -5 (kg/kW.s) of the specific fuel consumption. According to paired sample t-test, the difference in the engine performance is only experienced in the torque, which has a significant increase in the composition of the biodiesel+ED by 0.07 gr mixture.

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“…It has a chloroform-like smell and a spicy taste and it is An important handicap of this procedure was the very early verification of the economic difficulty of carrying out this process on an industrial scale, due to the high price of PPL lipases, even with their use in immobilized form, which allows their reuse. Therefore, in the last decade, a high number of low-cost lipases, both in free as well as immobilized forms have been evaluated, demonstrating in all cases the technical efficiency of the process [272][273][274][275][276][277][278][279][280][281][282][283]. However, the economic cost of the process is still the main difficulty to guarantee the economic viability at high production level.…”

Section: Biodiesel-like Biofuels That Integrate Glycerol As Monoglycementioning

confidence: 99%

Biodiesel at the Crossroads: A Critical Review

et al. 2019

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The delay in the energy transition, focused in the replacement of fossil diesel with biodiesel, is mainly caused by the need of reducing the costs associated to the transesterification reaction of vegetable oils with methanol. This reaction, on an industrial scale, presents several problems associated with the glycerol generated during the process. The costs to eliminate this glycerol have to be added to the implicit cost of using seed oil as raw material. Recently, several alternative methods to convert vegetable oils into high quality diesel fuels, which avoid the glycerol generation, are being under development, such as Gliperol, DMC-Biod, or Ecodiesel. Besides, there are renewable diesel fuels known as “green diesel”, obtained by several catalytic processes (cracking or pyrolysis, hydrodeoxygenation and hydrotreating) of vegetable oils and which exhibit a lot of similarities with fossil fuels. Likewise, it has also been addressed as a novel strategy, the use of straight vegetable oils in blends with various plant-based sources such as alcohols, vegetable oils, and several organic compounds that are renewable and biodegradable. These plant-based sources are capable of achieving the effective reduction of the viscosity of the blends, allowing their use in combustion ignition engines. The aim of this review is to evaluate the real possibilities that conventional biodiesel has in order to success as the main biofuel for the energy transition, as well as the use of alternative biofuels that can take part in the energy transition in a successful way.

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Evaluation of Lipases from Wild Microbial Strains as Biocatalysts in Biodiesel Production

Cited by 6 publications

References 29 publications

Monomyristin and Monopalmitin Derivatives: Synthesis and Evaluation as Potential Antibacterial and Antifungal Agents

Monomyristin and Monopalmitin Derivatives: Synthesis and Evaluation as Potential Antibacterial and Antifungal Agents

Engine Performance Using Blended Fuels of Biodiesel and Eco Diesel

Biodiesel at the Crossroads: A Critical Review

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