Evaluation of lipid peroxidation as a toxicity bioassay for plants exposed to copper

Baryla, Aurore; Laborde, Cécile; Montillet, Jean‐Luc; Triantaphylidès, C.; Chagvardieff, P.

doi:10.1016/s0269-7491(99)00232-8

Cited by 101 publications

(54 citation statements)

References 14 publications

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“…The determination of MDA content is widely used as a reliable tool to detect the oxidative stress hazard by estimating the formation of lipid peroxides in biological material (Loureiro et al, 2006;Taulavuori et al, 2001;Zielinska et al, 2001). Furthermore, the formation of ROS and an increased MDA production were observed in plants exposed to different heavy metals as Cr, Pb, Cu and Zn under laboratory conditions (Aravind and Prasad, 2003;Baryla et al, 2000;Sinha et al, 2005;Verma and Dubey, 2003).…”

Section: Introductionmentioning

confidence: 97%

Toxicity assessment of contaminated soils from a mining area in Northeast Italy by using lipid peroxidation assay

Wahsha

Bini

Fontana

et al. 2012

Journal of Geochemical Exploration

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Contamination by heavy metals in soils may strongly affect the environmental quality. Lipid peroxidation caused by heavy metals in plants was investigated as a relevant bioassay of toxicity. Soils and wild plants (dandelion and willow) were collected from an abandoned mine area in northeast Italy, and the concentration of different heavy metals (Ni, Cr, Cu, Pb, Zn, Fe and Mn) were measured and analyzed. Soils affected by mining activities presented total Zn, Cu, and Pb concentrations (2566, 3975, 20,815 mg kg −1 respectively) above toxic thresholds, and 58% for Fe. Heavy metal-induced oxidative stress was evidenced by the generation of reactive radicals, followed by an increase in malondialdehyde (MDA) production up to 41.64 μM in willow leaves. We found that MDA concentration in plant tissues differed significantly among species and plant organs. The higher concentration of metal in soil corresponded with the higher concentration of MDA in the plant. The combined results of metal concentration, MDA content and translocation coefficients in plants show that the investigated plants are rather highly tolerant towards environmental pollution. This suggests that they could be useful in phytoremediation of metal contaminated sites.

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Section: Introductionmentioning

confidence: 97%

Toxicity assessment of contaminated soils from a mining area in Northeast Italy by using lipid peroxidation assay

Wahsha

Bini

Fontana

et al. 2012

Journal of Geochemical Exploration

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“…This consists of the exaggerated generation of free radicals (hydroxyl radical, OH -; phenoxy radicals, RO -; peroxy radicals, ROO -) and other ROS (superoxide radical anion, O 2 -; singlet oxygen, 1 O 2 ; hydrogen peroxide, H 2 O 2 ) (Posmyk et al, 2009). The generation of ROS is considered to be a primary event under a variety of stress conditions (Baryla et al, 2000). In order to control the level of ROS and to protect cells under stress conditions, plant tissues are stimulated to produce several enzymes for scavenging ROS, including superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT), as well as a network of low molecular weight antioxidants (ascorbate, glutathione, phenolic compounds, tocopherol, and carotenoids) (Posmyk et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro

Jutamas¹,

Huang²,

Lee³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. In vitro grown cabbage (Brassica oleracea var. capitata) seedlings exposed to excess molybdenum (Mo) ions exhibited severely reduced plant growth at the cotyledonary stage. Adding 80 mM proline (Pro) to the Mo-treated medium could help 50% seedlings to overcome the toxicity and grow true leaves. Under excess Mo stress, seedlings accumulated blue/purple anthocyanin in their cotyledons and hypocotyls. The anthocyanin content under Mo with 40 mM Pro was 4-fold higher than the control medium, MS with 40 mM Pro. The presence of Pro in the excess-Mo condition reduced chlorophyll a, whereas the chlorophyll b content was much higher than the control media of MS with and without Pro. Moreover, supplementing various concentrations of Pro into the Mo-stressed condition promoted the seedlings with higher antioxidant enzyme activities of superoxide dismutase, ascorbate peroxidate, and catalase. In addition, genes in the anthocyanin biosynthesis and accumulation pathways, phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), flavonone 3-hydroxylase (F3H), leucoanthocyanidin dioxygenase (LDOX), and glutathione-S-transferase (GST), were all upregulated. Our study indicated that, under excess Mo stress, the antioxidant activity of cabbage seedlings was induced in an attempt to protect plants from the Mo-induced toxicity and exacerbated growth. Pro, on the other hand, functioned in producing higher antioxidant enzyme activity to partially help recover plant growth.

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“…In plants, nonenzymatic lipid peroxidation is known to increase during many abiotic stresses (Baryla et al, 2000) and in response to both bacterial and fungal pathogen attack (Muckenschnabel et al, 2001;Thoma et al, 2003). Linoleic (18:2) and linolenic (18:3) acids are highly abundant in plant membrane lipids and are the main reactants in nonenzymatic lipid peroxidation (Berger et al, 2001;Mueller, 2004;Weber et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Nonenzymatic Lipid Peroxidation Reprograms Gene Expression and Activates Defense Markers inArabidopsisTocopherol-Deficient Mutants

et al. 2006

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Tocopherols (vitamin E) are lipophilic antioxidants that are synthesized by all plants and are particularly abundant in seeds. Two tocopherol-deficient mutant loci in Arabidopsis thaliana were used to examine the functions of tocopherols in seedlings: vitamin e1 (vte1), which accumulates the pathway intermediate 2,3-dimethyl-5-phytyl-1,4-benzoquinone (DMPBQ); and vte2, which lacks all tocopherols and pathway intermediates. Only vte2 displayed severe seedling growth defects, which corresponded with massively increased levels of the major classes of nonenzymatic lipid peroxidation products: hydroxy fatty acids, malondialdehyde, and phytoprostanes. In the absence of pathogens, the phytoalexin camalexin accumulated in vte2 seedlings to levels 100-fold higher than in wild-type or vte1 seedlings. Similarly, gene expression profiling in wild-type, vte1, and vte2 seedlings indicated that increased levels of nonenzymatic lipid peroxidation in vte2 corresponded to increased expression of many defense-related genes, which were not induced in vte1. Both biochemical and transcriptional analyses of vte2 seedlings indicate that nonenzymatic lipid peroxidation plays a significant role in modulating plant defense responses. Together, these results establish that tocopherols in wild-type plants or DMPBQ in vte1 plants limit nonenzymatic lipid peroxidation during germination and early seedling development, thereby preventing the inappropriate activation of transcriptional and biochemical defense responses.

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Evaluation of lipid peroxidation as a toxicity bioassay for plants exposed to copper

Cited by 101 publications

References 14 publications

Toxicity assessment of contaminated soils from a mining area in Northeast Italy by using lipid peroxidation assay

Toxicity assessment of contaminated soils from a mining area in Northeast Italy by using lipid peroxidation assay

Proline partially overcomes excess molybdenum toxicity in cabbage seedlings grown in vitro

Nonenzymatic Lipid Peroxidation Reprograms Gene Expression and Activates Defense Markers inArabidopsisTocopherol-Deficient Mutants

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