Evaluation of liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry for the identification and quantification of desulphoglucosinolates

Abstract

The use of liquid chromatography–atmospheric pressure chemical ionisation–mass spectrometry has been investigated as a potential method for both the identification and quantification of desulphoglucosinolates. The results indicate that this methodology is particularly useful as a rapid technique for the confirmation of the identity of desulphoglucosinolates and has, in full scan mode, limits of detection at least an order of magnitude lower than is normally achieved by conventional high pressure liquid chromatography with UV detection. It has been demonstrated that, using a per‐deuterated desulphoglucosinolate as an internal standard, quantification could be achieved, but for accurate analysis at low concentrations it would be necessary to produce independent calibration curves for each desulphoglucosinolate present in the sample. Copyright © 2000 John Wiley & Sons, Ltd.

Simultaneous determination of hexabromocyclododecanes and tris (2,3-dibromopropyl) isocyanurate using LC–APCI-MS/MS

“…The APCI mechanism relies on a corona-needle discharge and the ions are produced by a charge transfer procedure. If competing ions are present and competes with the analyte ion for the charge, the analyte ion signal would be suppressed [23,24]. Therefore, in the present experiment, the ammonium acetate buffer was not used neither ESI nor APCI modes.…”

Rapid screening method for intact glucosinolates in Chinese medicinal herbs by using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry in negative ion mode

“…Both direct and indirect methods have been established for the determination of glucosinolates. Indirect analysis was based on the determination of enzymatic degradation products such as the released glucose,9–11 bisulfate ions12, 13 and desulfoglucosinolates 14–25. Gas chromatography (GC)14, 15 and reversed‐phase high‐performance liquid chromatography (RP‐HPLC)16–25 methods have been utilized for analyzing desulfoglucosinolates after their enzymatic desulfation.…”

“…Indirect analysis was based on the determination of enzymatic degradation products such as the released glucose,9–11 bisulfate ions12, 13 and desulfoglucosinolates 14–25. Gas chromatography (GC)14, 15 and reversed‐phase high‐performance liquid chromatography (RP‐HPLC)16–25 methods have been utilized for analyzing desulfoglucosinolates after their enzymatic desulfation. Direct methods are, however, important to characterize specific individual glucosinolates and intact glucosinolates which have been analyzed by RP‐HPLC using an ammonium acetate buffer26–28 or ion‐pairing reagents 29–34.…”

Molecular Genetics of Glucosinolate Biosynthesis in Brassicas: Genetic Manipulation and Application Aspects

“…Wt. # : molecular weight of desulfoglucosinolates, RF: response factor (Haughn et al, 1991;Griffiths et al, 2000;Brown et al, 2003). (Halkier & Gershenzon, 2006).…”