Evaluation of Lysinibacillus SP, isolated from Coptotermes curvignathus Gut, for the delignification of oil palm residues

Abstract

The application of ligninolytic bacteria and enzymes is a green pre-treatment alternative in the production of paper and biofuel from oil palm residues. In this study we investigated the ability of Lysinibacillus pakistanensis isolated from termite gut in degrading the lignin component of oil palm residues. The residues were biotreated with the bacterial strain in an aerated submerged fermentation system for 7 days at 30 , pH 7 and compared with untreated control. Enzyme activities were determined using specific substrates. Peak lignin peroxidase (377.6 U/L), manganese peroxidase (218.19 U/L), and laccase (405.4 U/L) activity were recorded after 4,4, and 5 days of incubation respectively, using oil palm leaf as substrates. Lignin loss of 4.5%, 5.7% and 6.6% in oil palm leaf, oil palm trunk and empty fruit bunch respectively was achieved after treatment with the microorganism. SEM images revealed structural changes in the cell wall of the residues. Pre-treatment with this bacterial strain has promising prospects of improving the efficiency of the pulping process in an environmentally safe manner.

Fermented Alfalfa Meal Instead of “Grain-Type” Feedstuffs in the Diet Improves Intestinal Health Related Indexes in Weaned Pigs

“…Genera that may contain large amounts of probiotics, such as Lactobacillus and Enterococcus , were enriched in the colon of FAM pigs. Interestingly, Lysinibacillus , a genus with a strong ability to degrade lignocellulose ( Ayeronfe et al, 2019 ), was also found more abundant in pigs fed FAM diet. It is speculated that the changed microbiota in colon of FAM pigs contributed to the fermentation ability more than CON pigs, which was confirmed by the markedly higher concentration of acetate in the colon of these pigs.…”