Evaluation of magnetic alginate beads as a solid support for positive selection of CD34+ cells

Abstract

Paramagnetic alginate beads with a 10-100 microns size range have been developed. These beads, when activated with chloroacetic anhydride and covalently coupled to avidin (30 micrograms/mg beads), were able to bind biotinylated goat anti-mouse (B-GAM) antibody (Ab). The beads with immobilized antibody were then used as a model system for the capture and non-enzymatic release of CD34+ (KG1a) cells. A maximum of 82% KG1a (average = 65 +/- 16.1%) cell capture, and 57% (average = 51 +/- 5.9%) cell release has been attained using this model system. Optimization of the system in terms of further bead size reduction, and in terms of developing a system to recover released cells with high purity, will make an excellent system for cell capture and nonenzymatic release.

RETRACTED ARTICLE: Synthesis, Structural and Magnetic Properties of Polypyrrole Coated Ni0.2Ca0.8Fe2O4 Nanocomposite

“…Thus by combining in a single material the electrical properties of ICPs and magnetic properties of nanoferrites, a multifunctional hybrid material could be developed that can find cutting edge technological applications in mechanical and electrical devices, magnetic resistive damping, loud speakers, seals, sensors, dampers, etc. They are being largely used for magneto caloric refrigeration [11], in EMI shielding etc.…”

Solvothermal Synthesis and Magnetic Property of Magnetic Chains Self‐assembled by Fe3O4 Microoctahedrons

“…With the development of the modern science, it is a great challenge to develop approaches to synthesize magnetic particles with suitable size, which helps to meet special need of biomedical fields. The research results indicate that various magnetic nano-or microparticles have been considered as ideal candidates for biological applications, such as protein and enzyme immobilization, cell isolation, DNA and RNA purification, immunoassay and guided site-specific drug delivery, [16][17][18][19][20] due to their good hydrophilic and biocompatible properties.…”

Nano Particles @ Calix Arenas Via Aqueous Solution

“…The matrixes play an important role in determining physical properties of the composite nanoparticle in addition to providing a means of particle dispersion 5 . [4][5][6][7] . Nano-Magnetites have shown great potential applications in the field of in vitro and in vivo biomedicine, including cellular therapy in cell labeling, separation and purification, target-drug delivery, and hyperthermia treatment of cancers.…”

“…Especially, nanoparticles of iron oxide are reported to be applicable as a material for use in drug delivery systems, magnetic resonance imaging, and cancer therapy [5][6][7][8] . Fe 3 O 4 is used as a catalyst in the Haber process and in the water gas shift reaction 12,13 .…”