Evaluation of Maize Inbred Lines for Iranian maize mosaic virus (IMMV) Resistance

Estakhr, A; Heidari, Bahram; Dadkhodaie, Ali; Izadpanah, K.

doi:10.9734/arrb/2015/20058

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seeds of MIMV-susceptible maize cultivar 704 [ 25 ] were sources from the Seed and Plant Improvement Research Division, Fars Research Center for Agriculture and Natural Resources, Shiraz, Iran, germinated in soil, and plants were grown in a greenhouse on a cycle of 16 h light at 30°C and 8 h dark at 25°C. Three days after seed germination, at the two-leaf stage, ten plants were exposed for 3 days to 20 viruliferous planthopper nymphs infected with MIMV isolate ‘Fars’ and had been maintained on MIMV-infected barley plants in an insect-proof cage.…”

Section: Methodsmentioning

confidence: 99%

Changes in maize transcriptome in response to maize Iranian mosaic virus infection

2018

View full text Add to dashboard Cite

BackgroundMaize Iranian mosaic virus (MIMV, genus Nucleorhabdovirus, family Rhabdoviridae) causes an economically important disease in maize and other gramineous crops in Iran. MIMV negative-sense RNA genome sequence of 12,426 nucleotides has recently been completed. Maize Genetics and Genomics database shows that 39,498 coding genes and 4,976 non-coding genes of maize have been determined, but still some transcripts could not be annotated. The molecular host cell responses of maize to MIMV infection including differential gene expression have so far not been elucidated.Methodology/Principal findingsComplementary DNA libraries were prepared from total RNA of MIMV-infected and mock-inoculated maize leaves and sequenced using Illumina HiSeq 2500. Cleaned raw transcript reads from MIMV-infected maize were mapped to reads from uninfected maize and to a maize reference genome. Differentially expressed transcripts were characterized by gene ontology and biochemical pathway analyses. Transcriptome data for selected genes were validated by real-time quantitative PCR.Conclusion/SignificanceApproximately 42 million clean reads for each treatment were obtained. In MIMV-infected maize compared to uninfected plants, 1689 transcripts were up-regulated and 213 transcripts were down-regulated. In response to MIMV infection, several pathways were activated in maize including immune receptor signaling, metabolic pathways, RNA silencing, hormone-mediated pathways, protein degradation, protein kinase and ATP binding activity, and fatty acid metabolism. Also, several transcripts including those encoding hydrophobic protein RCI2B, adenosylmethionine decarboxylase NAC transcription factor and nucleic acid binding, leucine-rich repeat, heat shock protein, 26S proteasome, oxidoreductases and endonuclease activity protein were up-regulated. These data will contribute to the identification of genes and pathways involved in plant-virus interactions that may serve as future targets for improved disease control.

show abstract

Section: Methodsmentioning

confidence: 99%