Evaluation of Malaria Urban Risk Using an Immuno-Epidemiological Biomarker of Human Exposure to Anopheles Bites

Traoré, Dipomin F.; Sagna, Aloïse; Adja, Akré Maurice; Zoh, Dounin D.; Lingué, Kouassi N.; Coulibaly, Issa; Tchiekoi, Bertin N’Cho; Assi, Serge-Brice; Poinsignon, Anne; Dagnogo, Mamadou; Remoué, Franck

doi:10.4269/ajtmh.17-0231

Cited by 15 publications

(31 citation statements)

References 25 publications

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“…Anopheles classically like small, open, sunlit, fresh stagnant water suggesting a mosaic of isolated pockets of Anopheles breeding sites in the urban context that may result of local hot spots of Anopheles exposure that could not have been identified. As mentioned above, the use of vector control strategies and/or sociological factors specific to the urban context may also explain the discrepancies observed between the two approaches [39]. Parasitological data indicated that Plasmodium prevalence and density (the gold standard to measure the transmission of malaria) were similar between the two sites, thereby suggesting that children were exposed similarly to malaria transmission.…”

Section: Discussionmentioning

confidence: 99%

“…The study was conducted in Bouaké (7° 69 N, 5° 03 W) located in the centre of Côte d'Ivoire. The study area, study design, and local malaria epidemiology have been previously described in detail [39]. Briefly, the climate is tropical humid with two seasons: the dry season runs from November through March, and the rainy season occurs from April to October.…”

Section: Study Sites and Populationmentioning

confidence: 99%

“…The initial cohort consisted of 212 children aged from 6 months to 14 years from two sites (a sub-set of a cohort of 508 children from 5 sites [39]) and enrolled in a cross-sectional study which was carried out during the rainy season (August 2014). Households and children were randomly selected and sociological, geographical, entomological, and clinical data were collected.…”

Section: Study Sites and Populationmentioning

confidence: 99%

“…Adult mosquitoes were collected in June and September 2014, as described [39]. In each of the two sites, six catching points, three indoor and three outdoor were used to collect mosquitoes by landing catches on adult volunteers for two consecutive nights (from 18.00. to 06.00).…”

Section: Mosquito Collectionmentioning

confidence: 99%

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Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites

Aka

Traoré

Sagna

et al. 2020

Malar J

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Background: In malaria-endemic areas, human populations are frequently exposed to immunomodulatory salivary components injected during mosquito blood feeding. The consequences on pathogen-specific immune responses are not well known. This study evaluated and compared the humoral responses specific to merozoite stage vaccine candidates of Plasmodium falciparum, in children differentially exposed to Anopheles bites in a natural setting. Methods:The cross-sectional study was carried out in Bouaké (Côte d'Ivoire) where entomological data and blood samples from children (0-14 years) were collected in two sites with similar malaria prevalence. Antibody (IgG, IgG1, IgG3) responses to PfAMA1 and PfMSP1 were evaluated by ELISA. Univariate and multivariate analysis were performed to assess the relationship between the immune responses to P. falciparum antigens and exposure to Anopheles bites in the total cohort and in each site, separately. The individual level of exposure to Anopheles bites was evaluated by quantifying specific IgG response to the Anopheles gSG6-P1 salivary peptide, which represents a proxy of Anopheles exposure.

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Section: Discussionmentioning

confidence: 99%

Section: Study Sites and Populationmentioning

confidence: 99%

Section: Study Sites and Populationmentioning

confidence: 99%

Section: Mosquito Collectionmentioning

confidence: 99%

See 2 more Smart Citations

Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites

Aka

Traoré

Sagna

et al. 2020

Malar J

Self Cite

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show abstract

“…Antibody titers to mosquito saliva are a proxy for the intensity of mosquito bites received by the individual. This biomarker has been used to assess the risk of malaria transmission in low-level exposure/transmission areas to accurately evaluate the effectiveness of interventions [10,11,12,13].…”

Section: Introductionmentioning

confidence: 99%

Stratifying Malaria Receptivity In Bangladesh Using Archived Rapid Diagnostic Tests

Sagna

Kibria

Naher

et al. 2020

Preprint

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Background Surveillance on low-density infections and exposure to vectors is crucial to understand where malaria elimination might be feasible, and where the risk of outbreaks is high. Archived rapid diagnostic tests (RDTs), used by national malaria control and elimination programs for clinical diagnosis, present a valuable, yet rarely used resource for in-depth studies on malaria epidemiology. Methods We screened 1,022 RDTs from two sub-Districts in Bangladesh (Alikadam and Kamalganj) by qPCR for low-density Plasmodium falciparum and Plasmodium vivax infections, and by ELISA for Anopheles salivary gland antibodies as a marker for exposure to vectors. Results Concordance between RDT and qPCR was moderate. qPCR detected 31/1022 infections compared to 36/1022 diagnosed by RDT. Exposure to Anopheles was significantly higher in Kamalganj despite low transmission, which could be explained by low bed net use. Conclusions Archived RDTs present a valuable source of antibodies for serological studies on exposure to vectors. In contrast, the benefit of screening archived RDTs to obtain a better estimate of test positivity is moderate. Kamalganj could thus be prone to outbreaks.

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Utility of plasma anti-gSG6-P1 IgG levels in determining changes in Anopheles gambiae bite rates in a rural area of Cameroon

Nguetsa,

Elanga-Ndille,

Essangui Same

et al. 2024

Sci Rep

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The applicability of the specific human IgG antibody response to Anopheles gambiae salivary Gland Protein-6 peptide 1 (gSG6-P1 salivary peptide) as a biomarker able to distinguish the level of exposure to mosquito bites according to seasonal variations has not yet been evaluated in Central African regions. The study aimed to provide the first reliable data on the IgG anti-gSG6-P1 response in rural area in Cameroon according to the dry- and rainy-season. Between May and December 2020, dry blood samples were collected from people living in the Bankeng village in the forest area of the Centre region of Cameroon. Malaria infection was determined by thick-blood smear microscopy and multiplex PCR. The level of IgG anti-gSG6-P1 response, was assessed by enzyme-linked immunosorbent assay. Anopheles density and aggressiveness were assessed using human landing catches. The prevalence of malaria infection remains significantly higher in the rainy season than in the dry season (77.57% vs 61.44%; p = 0.0001). The specific anti-gSG6-P1 IgG response could be detected in individuals exposed to few mosquito bites and showed inter-individual heterogeneity even when living in the same exposure area. In both seasons, the level of anti-gSG6-P1 IgG response was not significantly different between Plasmodium infected and non-infected individuals. Mosquito bites were more aggressive in the rainy season compared to the dry season (human biting rate-HBR of 15.05 b/p/n vs 1.5 b/p/n) where mosquito density was very low. Infected mosquitoes were found only during the rainy season (sporozoite rate = 10.63% and entomological inoculation rate-EIR = 1.42 ib/p/n). The level of IgG anti-gSG6-P1 response was significantly higher in the rainy season and correlated with HBR (p ˂ 0.0001). This study highlights the high heterogeneity of individual’s exposure to the Anopheles gambiae s.l vector bites depending on the transmission season in the same area. These findings reinforce the usefulness of the anti-gSG6-P1 IgG response as an accurate immunological biomarker for detecting individual exposure to Anopheles gambiae s.l. bites during the low risk period of malaria transmission in rural areas and for the differentiating the level of exposure to mosquitoes.

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Evaluation of Malaria Urban Risk Using an Immuno-Epidemiological Biomarker of Human Exposure to Anopheles Bites

Cited by 15 publications

References 25 publications

Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites

Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites

Stratifying Malaria Receptivity In Bangladesh Using Archived Rapid Diagnostic Tests

Utility of plasma anti-gSG6-P1 IgG levels in determining changes in Anopheles gambiae bite rates in a rural area of Cameroon

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