Evaluation of mast cell counts and microvessel density in reactive lesions of the oral cavity

Abstract

Background. Reliable immunohistochemical assays to assess the definitive role of mast cells (MCs) and angiogenesis in the pathogenesis of oral reactive lesions are generally not available. The aim of the present study was to evaluate mast cell counts (MCC) and microvessel density (MVD) in oral reactive lesions and determine the correlation between MCC and MVD.Methods. Seventy-five cases of reactive lesions of the oral cavity, including pyogenic granuloma, fibroma, peripheral giant cell granuloma, inflammatory fibrous hyperplasia, peripheral ossifying fibroma (15 for each category) were immunohisto-chemically stained with MC tryptase and CD31. Fifteen cases of normal gingival tissue were considered as the control group. The mean MCC and MVD in superficial and deep connective tissues were assessed and total MCC and MVD was computed for each lesion.Results. Statistically significant differences were observed in MCC and MVD between the study groups (P < 0.001). MC tryptase and CD31 expression increased in the superficial connective tissue of each lesion in comparison to the deep con-nective tissue. A significant negative correlation was not found between MCC and MVD in oral reactive lesions (P < 0.001, r = -0.458). Conclusion. Although MCs were present in the reactive lesions of the oral cavity, a direct correlation between MCC and MVD was not found in these lesions. Therefore, a significant interaction between MCs and endothelial cells and an active role for MCs in the growth of oral reactive lesions was not found in this study.

Oral pyogenic granuloma: An 18‐year retrospective clinicopathological and immunohistochemical study

“…The outcome of mutual mast cell‐fibroblast interactions promotes granulation tissue formation, a hallmark of PG, 18 which encouraged us to evaluate the mast cells numbers in both histopathological subtypes. Several authors 19 have found a positive correlation between the number of microvessels and mast cells in a group of so‐called reactive lesions, including LCH, fibrous hyperplasia, inflammatory fibrous hyperplasia, and peripheral giant cell lesion, while others found a negative correlation 20 . In our study, we found a positive, yet not significant correlation between microvessels and mast cells, similar to previous results, 18 and no difference in the number of mast cells between groups.…”

Histochemical and immunohistochemical investigation of the number and localization of mast cells in the feline tongue

“…Kouhsoltani et al. (2016) also showed that MC T expression was higher in gingival tissue below the epithelial surface compared to deep connective tissue. There are few studies in the literature on the histochemistry of MCs enzymes for neutral serine proteases in the tongue.…”

“…In addition, MC T s have been identified in the skin of felines in both the subepidermal and deep dermis (Noli et al, 2003). Kouhsoltani et al (2016)…”

Oral Pyogenic Granuloma: A Narrative Review

“…The outcome of mutual mast cell-fibroblast interactions promotes granulation tissue formation, a hallmark of PG [55], which encouraged us to evaluate the mast cells numbers in both histopathological subtypes. Several authors [56], have found a positive correlation between the number of micro vessels and mast cells in a group of so-called reactive lesions, including LCH, fibrous hyperplasia, inflammatory fibrous hyperplasia, and peripheral giant cell lesions, while others found a negative correlation [57]. The major basic protein of eosinophils has proangiogenic effects and may participate in inflammation and tissue remodeling.…”