Evaluation of matrix effect in one‐dimensional and comprehensive two‐dimensional liquid chromatography for the determination of the phenolic fraction in extra virgin olive oils

Arena, Katia; Cacciola, Francesco; Rigano, Francesca; Dugo, Paola

doi:10.1002/jssc.202000169

Cited by 22 publications

(14 citation statements)

References 43 publications

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“…Furthermore, the values of LODs obtained ranged from 0.24 mg/L to 0.39 mg/L for apigenin and epicatechin, respectively; whereas, LOQs values were lower than 1.13 mg/L (Table 1). It has been demonstrated that LODs values in LC×LC are higher than 1D-LC, due to chromatographic dilution [28]. However, the LODs results attained in this work by LC×LC-PDA are comparable with the ones reported by a recent work at least for five compounds, namely catechin, epicatechin, quercetrin, quercetin, and kaempferol, analyzed by LC-UV [29].…”

Section: Development and Validation Of The Quantitative Methodssupporting

confidence: 73%

Polyphenolic compounds with biological activity in guabiroba fruits (Campomanesia xanthocarpaBerg.) by comprehensive two‐dimensional liquid chromatography

et al. 2020

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This study aimed to evaluate the polyphenolic composition along with the biological activity of guabiroba (Campomanesia xanthocarpa Berg.) fruits using comprehensive two-dimensional liquid chromatography (LC × LC). A simplex centroid design comprising three solvents (methanol, 2% acetic acid, and acetonitrile) was used to optimize the extraction mixture for polyphenols from ripe and unripe guabiroba fruits. A quantitative LC × LC platform was proposed to characterize the guabiroba extracts using a RP-Amide column and a C18 column in the first and second dimensions, respectively. Antidiabetic properties, using in vitro enzyme assay models and in vivo antioxidant activity with the eukaryote model Saccharomyces cerevisiae, was measured. Total phenolics compounds were more efficiently extracted with 2% acetic acid solution and acetonitrile (50:50, v/v). A total of 37 different compounds were identified and quantified using the proposed LC × LC method (linearity ranging from 0.9990 to 0.9994, intra-and interday precision from 0.40 to 10.57% and, accuracy from 81.89 to 108.98%). Significant differences were observed between ripe and unripe guabiroba fruits, especially for the compounds geraldone and methyl galangin isomer. Guabiroba fruits showed significant antidiabetic and antioxidant properties and may be potentially adopted as part of dietary strategies in the management of early stages of type 2 diabetes and associated complications.

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Section: Development and Validation Of The Quantitative Methodssupporting

confidence: 73%

Polyphenolic compounds with biological activity in guabiroba fruits (Campomanesia xanthocarpaBerg.) by comprehensive two‐dimensional liquid chromatography

et al. 2020

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“…With regards to corrected peak capacity 2D n corr values, incorporating under-sampling [ 40 ] and A O values [ 38 ], the highest values were obtained for Samples 4 (1161) and 3 (1004), respectively. When comparing these values with previously reported ones, these were undoubtedly higher than RP-LC×RP-LC set-ups (695 in Wong et al [ 22 ], 461–633 in Arena et al [ 23 ], 404–639 in Arena et al [ 26 ]), despite slightly lower than similar HILIC×RP-LC set-up with active flow modulation (1605–1830 in Toro-Uribe et al [ 29 ]).…”

Section: Resultsmentioning

confidence: 49%

“…In this work the analysis of the polyphenolic compounds in R. coriaria samples was carried out by HILIC×RP-LC-PDA-ESI/MS. So far, most of the applications on polyphenols in food and natural products have been carried out on RP-LC×RP-LC [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ], despite applications of HILIC×RP-LC also being reported [ 28 , 29 , 30 ]. Prior to either RP-LC×RP-LC or HILIC×RP-LC analysis, an optimization of the single separations must be carried out [ 31 , 32 , 33 , 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Characterization of Rhus coriaria L. Extracts by Headspace Solid-Phase Micro Extraction Gas Chromatography, Comprehensive Two-Dimensional Liquid Chromatography, and Antioxidant Activity Evaluation

et al. 2022

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Rhus coriaria L. (Anacardiaceae), commonly known as sumac, has been used since ancient times for many different applications, and nowadays is used mostly as a spice obtained from its in the Mediterranean and the Middle ground fruits and employed for flavoring and garnishing food, predominantly Eastern regions. Traditionally, sumac has been also used in popular medicine for the treatment of many ailments including hemorrhoids, wound healing, diarrhea, ulcers, and eye inflammation. Sumac drupes are indeed rich in various classes of phytochemicals including organic acids, flavonoids, tannins, and others, which are responsible of their powerful antioxidant capacity, from which treatment of many common diseases such as cardiovascular disease, diabetes, and cancer could benefit. In this work we evaluated the influence of fruit ripeness, conservation, and processing. To this aim, a phytochemical characterization of six different samples of Rhus coriaria L. was carried out. Specifically, headspace solid-phase micro extraction gas chromatography coupled to mass spectrometry and comprehensive two-dimensional liquid chromatography coupled to photodiode array and mass spectrometry detection, were employed. A total of 263 volatile compounds, including terpene hydrocarbons, acids, and aldehydes, as well as 83 polyphenolic compounds, mainly gallic acid derivatives, were positively identified. All samples showed a significant antioxidant activity by means of oxygen radical absorbance capacity, in line with their polyphenolic content and composition. Such findings set a solid ground to support the utilization of this plant as an attractive target for novel nutraceutical approaches and for drug discovery.

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“…LOD values are instead one or two orders of magnitude lower (Bartella et al, 2019(Bartella et al, , 2020 using multiple sector MS/MS instruments. Recent high-resolution equipment comes very close to these letter levels of sensitivity (Arena et al, 2020;Bajoub et al, 2020;Dini et al, 2020;Kanakis et al, 2013). In both approaches (MS/MS or HRMS), the increase in sensitivity is achieved by reducing the background noise due to interfering ions passing through the analyzer that are finally perceived by the detector.…”

Section: Ms Based Approaches For Bio-phenol Determination and The Com...mentioning

confidence: 99%

Bio‐phenols determination in olive oils: Recent mass spectrometry approaches

Bongiorno

Stefano

Indelicato

et al. 2021

Mass Spectrometry Reviews

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Extra virgin olive oil (EVOO) is largely used in Mediterranean diet, and it is also worldwide apprised not only for its organoleptic properties but also for its healthy effects mainly attributed to the presence of several naturally occurring phenolic and polyphenolic compounds (bio-phenols). These compounds are characterized by the presence of multiple phenolic groups in more or less complex structures.Their content is fundamental in defining the healthy qualities of EVOO and consequently the analytical methods for their characterization and quantification are of current interest. Traditionally their determination has been conducted using a colorimetric assay based on the reaction of Folin-Ciocalteu (FC) reagent with the functional hydroxy groups of phenolic compounds. Identification and quantification of the bio-phenols in olive oils requires certainly more performing analytical methods. Chromatographic separation is now commonly achieved by HPLC, coupled with spectrometric devices as UV, FID, and MS. This last approach constitutes an actual cutting-edge application for bio-phenol determination in complex matrices as olive oils, mostly on the light of the development of mass analyzers and the achievement of high resolution and accurate mass measurement in more affordable instrument configurations. After a short survey of some rugged techniques used for bio-phenols determination, in this review have been described the most recent mass spectrometry-based methods, adopted for the analysis of the bio-phenols in EVOOs. In particular, the sample handling and the results of HPLC coupled with low-and high-resolution MS and MS/MS Mass Spec Rev.

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Evaluation of matrix effect in one‐dimensional and comprehensive two‐dimensional liquid chromatography for the determination of the phenolic fraction in extra virgin olive oils

Cited by 22 publications

References 43 publications

Polyphenolic compounds with biological activity in guabiroba fruits (Campomanesia xanthocarpaBerg.) by comprehensive two‐dimensional liquid chromatography

Polyphenolic compounds with biological activity in guabiroba fruits (Campomanesia xanthocarpaBerg.) by comprehensive two‐dimensional liquid chromatography

Phytochemical Characterization of Rhus coriaria L. Extracts by Headspace Solid-Phase Micro Extraction Gas Chromatography, Comprehensive Two-Dimensional Liquid Chromatography, and Antioxidant Activity Evaluation

Bio‐phenols determination in olive oils: Recent mass spectrometry approaches

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