Evaluation of MChip with Historic Subtype H1N1 Influenza A Viruses, Including the 1918 “Spanish Flu” Strain

Moore, Chris; Smagala, James A.; Smith, Catherine; Dawson, Erica D.; Cox, Nancy J.; Kuchta, Robert D.; Rowlen, Kathy L.

doi:10.1128/jcm.01089-07

Cited by 16 publications

(9 citation statements)

References 12 publications

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“…Microarray assays, which are capable of detecting multiple targets in parallel, open new possibilities, including the in-depth analysis of virus isolates within a short turnaround time. Recently, microarray assays have been described as valuable methods for the detection, differential diagnosis, and subtyping (of the H1, H2, H3, H5, N1, and N2 subtypes) of influenza A viruses with relevance to human disease (9,10,18,20,21,22,27,28,38,39), as well as for HA and NA subtyping (23,24,32,33).…”

mentioning

confidence: 99%

Design and Validation of a Microarray for Detection, Hemagglutinin Subtyping, and Pathotyping of Avian Influenza Viruses

et al. 2009

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mentioning

confidence: 99%

Design and Validation of a Microarray for Detection, Hemagglutinin Subtyping, and Pathotyping of Avian Influenza Viruses

et al. 2009

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“…In contrast, there are few assays currently available that couple nucleic acid amplification with a microarray-based scheme for detection. Mchip and Flu-Chip (19,20,33) are examples of such tests; however, these assays are available only as research use only (RUO) assays and are not widely used in clinical labs. The xTAG RVP (Luminex) is an FDA-cleared product capable of simultaneous detection of 10 different viruses, including two influenza A virus subtypes (16,18).…”

Section: Discussionmentioning

confidence: 99%

Real-Time Detection of Influenza A, Influenza B, and Respiratory Syncytial Virus A and B in Respiratory Specimens by Use of Nanoparticle Probes

Jannetto

Buchan

Vaughan³

et al. 2010

J Clin Microbiol

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Seasonal epidemics of influenza and respiratory syncytial virus are responsible for significant morbidity and mortality worldwide. Infrequently, novel or reemergent strains of influenza A virus have caused rapid, severe global pandemics resulting in millions of fatalities. The ability to efficiently and accurately detect and differentiate respiratory viruses is paramount for effective treatment, infection control, and epidemiological surveillance. We evaluated the ability of two FDA-cleared nucleic acid-based tests, the semiautomated respiratory virus nucleic acid test (VRNAT) and the fully automated respiratory virus nucleic acid test SP (RVNAT SP ) (Nanosphere Inc., Northbrook, IL) to detect influenza A virus, influenza B virus, and respiratory syncytial virus A and B (RSV A/B) from clinical nasopharyngeal swab specimens. Detection of viral RNA in both tests is based on nucleic acid amplification followed by hybridization to capture probes immobilized on a glass slide. A novel technology utilizing gold nanoparticle-conjugated probes is utilized to detect the presence of captured target DNA. This microarray-based approach to detection has proven to be more sensitive than the traditional culture/direct fluorescent-antibody assay (DFA) method for detecting RSV and influenza viruses in clinical specimens, including the novel 2009 H1N1 strain. Specifically, we report 98.0% sensitivity and 96.5% specificity for the VRNAT compared to culture/DFA. Further, the VRNAT detected virus in an additional 58% of specimens that were culture negative. These data were confirmed using bidirectional sequencing. Evaluation of the fully automated RVNAT SP , which is built on the same detection technology as the VRNAT but contains an updated processor enabling complete automation, revealed the two tests to be functionally equivalent. Thus, the RVNAT SP is a fully automated sample-to-result test capable of reliable detection of select respiratory viruses directly from clinical specimens in 3.5 h.Influenza virus and respiratory syncytial virus (RSV) infect millions of individuals annually. Influenza virus is typically associated with infections of the upper respiratory tract and can cause mild to severe illness, with symptoms including abrupt onset of fever, malaise, severe myalgia, and a nonproductive cough. The bulk of illness is attributed seasonal epidemics of influenza virus types A and B, while type C is far less prevalent and rarely causes severe disease (9, 23). Type A influenza virus is often associated with more-severe symptoms, morbidity, and mortality owing to a greater genetic diversity typified by chromosomal reassortment between human and avian viruses. Such genetic reassortments are responsible for the emergence of novel subtypes of influenza A virus, including the infamous H1N1 Spanish flu virus of 1918 and the more recent 2009 H1N1 or "swine flu" virus (26).RSV is capable of causing severe symptoms in infants, young children, and immunocompromised individuals (9,24,28) and is the leading cause of hospitalization in ch...

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“…This method was then evaluated with 16 historic subtype H1N1 influenza A viruses, including A/Brevig Mission/1/1918. MChip successfully detected the matrix gene segments from all 16 viruses [48]. Townsend et al developed a low-density oligonucleotide microarray (FluChip-55 [InDevR]) for the subtyping of H1N1, H3N2 and H5N1 [46].…”

Section: Nucleic Acid Sequence-based Methodsmentioning

confidence: 99%

Methods for molecular surveillance of influenza

Wang

Taubenberger

2010

Expert Review of Anti-infective Therapy

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Molecular-based techniques for detecting influenza viruses have become an integral component of human and animal surveillance programs in the last two decades. The recent pandemic of the swineorigin influenza A virus (H1N1) and the continuing circulation of highly pathogenic avian influenza A virus (H5N1) further stress the need for rapid and accurate identification and subtyping of influenza viruses for surveillance, outbreak management, diagnosis and treatment. There has been remarkable progress on the detection and molecular characterization of influenza virus infections in clinical, mammalian, domestic poultry and wild bird samples in recent years. The application of these techniques, including reverse transcriptase-PCR, real-time PCR, microarrays and other nucleic acid sequencing-based amplifications, have greatly enhanced the capability for surveillance and characterization of influenza viruses.

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Evaluation of MChip with Historic Subtype H1N1 Influenza A Viruses, Including the 1918 “Spanish Flu” Strain

Cited by 16 publications

References 12 publications

Design and Validation of a Microarray for Detection, Hemagglutinin Subtyping, and Pathotyping of Avian Influenza Viruses

Design and Validation of a Microarray for Detection, Hemagglutinin Subtyping, and Pathotyping of Avian Influenza Viruses

Real-Time Detection of Influenza A, Influenza B, and Respiratory Syncytial Virus A and B in Respiratory Specimens by Use of Nanoparticle Probes

Methods for molecular surveillance of influenza

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