Evaluation of measurement uncertainty in the determination of jasmonic acid in Lemna minor L. by liquid chromatography with fluorescence detection

Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction–Derivatization

“…Complicated operations tended to cause high system errors, as poor recoveries for labile GA 3 were usually observed. ,,, Among the reported methods, HPLC-FLD with derivatization technique provided more accurate determination with excellent recoveries, ,, probably because of the stabilizing effect of derivatization (see Table S2). In the present work, instead of using conventional SPE or LLME, this method is improved by integrating the extraction and derivatization in a closed Teflon vessel, thus allowing the pretreatment to be finished with a facile operation in 1.5 h. Moreover, the microwave oven can contain 10 Teflon vessels at least, which makes this method more suitable for batch analysis than conventional 10 sets of instruments equipped with an oil bath or a sand bath, demonstrating the remarkable capacity, efficiency, and convenience relative to conventional methods. − With the derivatization technique, the detection limits are improved and, more importantly, labile analytes can be stabilized via the molecular modification. Nevertheless, in view of the susceptibility of GA 3 , further identification by online MS/MS was followed.…”

“…Endogenous PGRs occur naturally in a complex matrix and influence physiological processes at low concentrations . Among the reported methods for PGR determination, − unsatisfactory low sensitivity, ,,,− , a poor selectivity, ,,, limited PGRs, − ,,,− ,, and applicability ,,,, were usually found. Due to the weak chromophore in molecules, PGRs at trace levels were difficult to detect directly without any pretreatment.…”

“…Although preconcentration could lower detection limits, the interfering components would be also concentrated together. In this case, multistep manual operations such as solid-phase extraction (SPE) ,,,− or liquid–liquid microextraction (LLME) ,, were usually required. However, these operations came with certain limitations, coelution of interfering species, high sample carry-over, emulsion formation, and analytes loss, thus tending to cause large error and low recovery. ,,,,,,, More significantly, GA 3 , NAA, and IAA were susceptible to light, temperature, or oxygen, ,− which increased the difficulty for the accurate determination of PGRs with multiple operations.…”

“…In this case, multistep manual operations such as solid-phase extraction (SPE) ,,,− or liquid–liquid microextraction (LLME) ,, were usually required. However, these operations came with certain limitations, coelution of interfering species, high sample carry-over, emulsion formation, and analytes loss, thus tending to cause large error and low recovery. ,,,,,,, More significantly, GA 3 , NAA, and IAA were susceptible to light, temperature, or oxygen, ,− which increased the difficulty for the accurate determination of PGRs with multiple operations. In addition, to both the experimenter and the environment, tedious operation with high reagent consumption was always unsatisfactory.…”

Simultaneous Determination of Salicylic Acid, Jasmonic Acid, Methyl Salicylate, and Methyl Jasmonate fromUlmus pumilaLeaves by GC-MS

“…However, it is difficult to produce monoclonal antibodies, so not easy to popularize. Besides, combined with triple quadrupole mass spectrometer [ 7 – 10 ], time-of-flight mass spectrometer, and fluorescence detector [ 11 , 12 ], HPLC can measure jasmonic acid and salicylic acid levels simultaneously. Zhang et al have determined jasmonic acid level from extract of Hevea brasiliensis bark by using capillary electrophoresis laser-induced fluorescence detection method [ 13 ].…”