Evaluation of mesenchymal stem cells in treatment of infertility in male rats

Hassan, Amal I.; Alam, Sally S.

doi:10.1186/scrt521

Cited by 46 publications

(44 citation statements)

References 67 publications

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“…The exact mechanism of Lead-induced toxicity is fully not understood, as there has been a record showing a linkage between oxidative stress and Lead toxicity. When Lead is administered the mechanism involved in distorting testes spermatogenesis involves the oxidative stress ions that cause depletion of ROS scavengers, lipid peroxidation and reduction of reproductive system antioxidant enzyme activities 15 .…”

Section: Discussionmentioning

confidence: 99%

The Effect of Antioxidant (Gallic acid) on the Testes of Lead Acetate Induced Wistar Rat

Bello

Idris

2018

Toxicol. Environ. Health Sci.

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Section: Discussionmentioning

confidence: 99%

The Effect of Antioxidant (Gallic acid) on the Testes of Lead Acetate Induced Wistar Rat

Bello

Idris

2018

Toxicol. Environ. Health Sci.

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“…Bone marrow-derived MSCs (BMSCs) could reduce the DNA fragmentation and ROS reaction to alleviate testicular toxicity induced by lead nitrate (Hassan & Alam, 2014). MSCs have been documented to those rodent experimental azoospermia.…”

Section: Introductionmentioning

confidence: 99%

“…MSCs have been documented to those rodent experimental azoospermia. Bone marrow-derived MSCs (BMSCs) could reduce the DNA fragmentation and ROS reaction to alleviate testicular toxicity induced by lead nitrate (Hassan & Alam, 2014). Umbilical cord-derived MSCs also enhance expression of meiotic-specific genes to restore spermatogenesis induced by busulfan .…”

mentioning

confidence: 99%

Mesenchymal stem cell‐secreted factors delayed spermatogenesis injuries induced by busulfan involving intercellular adhesion molecule regulation

et al. 2019

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The present study was designed to investigate the therapeutic effect of bone marrow MSC‐derived factors on gonadotropic toxicity induced by busulfan in vivo. The conditioned media (CM) was obtained from MSCs in serum‐free incubation for 48 hr and concentrated ~25‐fold by ultrafiltration. The CM of HEK 293 cells was treated as control (293‐CM). MSC‐CM was injected into busulfan mice via caudal veins after 1 day of busulfan treatment for 2 weeks (200 μl per dose/twice weekly）. Compared to the 293‐CM group, testicular injury was delayed in MSC‐CM group, including reduced vacuolations of cells in the basal compartment of the seminiferous epithelium and detachment of cells from basement membrane. Apoptotic spermatogenic cells were significantly decreased in MSC‐CM group (p ＜ 0.05). Interesting N‐cadherin，ICAM‐1 and P‐cadherin expressions significantly increased in MSC‐CM group, while occludin, ZO‐1 and connexin 43 expressions showed no difference among MSC‐CM, 293‐CM and busulfan groups. Present results suggest MSC‐secreted factors protect spermatogenesis impairment after busulfan treatment by reducing the apoptosis of spermatogenic cells and enhancing intercellular adhesion molecule expressions.

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“…Such observations suggest that MSCs might possess a role in recovering spermatogenesis via two mechanisms: 1) MSC transformation into sperm, or 2) preservation of the spermatogonial cells. Hassan and Alam (2014) showed that a functional rich source for treating infertility is through MSCs (Hassan and Alam, 2014). Yazawa et al (2006) confirmed that MSCs possess the ability to distinguish into steroidogenic cells, like Leydig cells, in vivo and in vitro (Yazawa et al, 2006).…”

Section: El-wakf Et Al (2009) (Elmentioning

confidence: 79%

“…Moreover, the precise reactions of MSCs to oxidative damage may play a vital role in control of tissue homeostasis in addition to renewal of organs following oxidative damage (Burova et al, 2013;Hassan and Alam, 2014).…”

Section: Discussionmentioning

confidence: 99%

The potential role of mesenchymal stem cells in a hypoxia model induced by sodium nitrite in testes of male albino rats

et al. 2017

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Introduction: The present work aims to examine the possible role of stem cells on biochemical markers and histopathological alterations of hypoxia caused by sodium nitrite (NaNO 2 ) toxicity in testes of male rats. Methods: In this study, 96 adult male albino rats were divided into 6 groups (16 rats each). Group 1 (G1) was the control group and received distilled H 2 O. Group 2 (G2) received daily NaNO 2 (35 m/kg bwt/ day) via subcutaneous injection for 3 weeks. Group 3 (G3) received NaNO 2 for 2 weeks and were then injected once with 2*10 6 mesenchymal stem cells (MSCs) intravenously and sacrificed 4 weeks later. Group 4 (G4) received NaNO 2 for 2 weeks and were then injected with 2*10 6 MSCs followed by daily NaNO 2 injection for 1 week; rats in G4 were sacrificed 4 weeks from MSCs treatment. Group 5 (G5) rats were treated with NaNO 2 for 2 weeks and then left to recover for 4 weeks. Finally, Group 6 (G6) rats were treated with NaNO 2 for 3 weeks and left to recover for 3 weeks, after which point they were sacrificed. Results: The results showed that NaNO 2 caused oxidative damage and histopathological alterations in the rat testes, as well as increased the levels of testes malondialdehyde (MDA), nitric oxide (NO) and DNA fragmentation percentage (DNA F %). Moreover, NaNO 2 decreased the elevated activities of testes catalase (CAT) and total antioxidant activity (TAA), in comparison to the control group. The histological results illustrated different distortions, vacuolization and lipid accumulations in interlobular space as well as diminution of inter cellular germ cell layers, absence of Leydig cells, irregular basement membrane of tubule, and separation within spermatogenic cells. In

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Evaluation of mesenchymal stem cells in treatment of infertility in male rats

Abstract: Introduction: The present study aimed to elucidate the therapeutic effects of mesenchymal stem cells (MSCs) derived from the bone marrow of rats (BM) against toxic effects of lead (Pb) on the male gonads of experimental rats.

Cited by 46 publications

References 67 publications

The Effect of Antioxidant (Gallic acid) on the Testes of Lead Acetate Induced Wistar Rat

The Effect of Antioxidant (Gallic acid) on the Testes of Lead Acetate Induced Wistar Rat

Mesenchymal stem cell‐secreted factors delayed spermatogenesis injuries induced by busulfan involving intercellular adhesion molecule regulation

The potential role of mesenchymal stem cells in a hypoxia model induced by sodium nitrite in testes of male albino rats

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