Evaluation of mesophilic Burkholderia sacchari, thermophilic Schlegelella thermodepolymerans and halophilic Halomonas halophila for polyhydroxyalkanoates production on model media mimicking lignocellulose hydrolysates

Kouřilová, Xenie; Nováčková, Ivana; Koller, Martin; Obruča, Stanislav

doi:10.1016/j.biortech.2021.124704

Cited by 29 publications

(21 citation statements)

References 28 publications

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“…However, the relevant enzymatic and conversion studies should also consider the possible presence of inhibitors that could influence the enzymatic rate [ 80 ]. Overall, the use of LF in PHA production is still ongoing research ( Table 5 ) [ 101 , 102 , 103 ].…”

Section: Carbon Sources or Feedstocks For Pha Productionmentioning

confidence: 99%

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Vigneswari

Noor

Amelia

et al. 2021

Life

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Polyhydroxyalkanoates (PHA) are biodegradable polymers that are considered able to replace synthetic plastic because their biochemical characteristics are in some cases the same as other biodegradable polymers. However, due to the disadvantages of costly and non-renewable carbon sources, the production of PHA has been lower in the industrial sector against conventional plastics. At the same time, first-generation sugar-based cultivated feedstocks as substrates for PHA production threatens food security and considerably require other resources such as land and energy. Therefore, attempts have been made in pursuit of suitable sustainable and affordable sources of carbon to reduce production costs. Thus, in this review, we highlight utilising waste lignocellulosic feedstocks (LF) as a renewable and inexpensive carbon source to produce PHA. These waste feedstocks, second-generation plant lignocellulosic biomass, such as maize stoves, dedicated energy crops, rice straws, wood chips, are commonly available renewable biomass sources with a steady supply of about 150 billion tonnes per year of global yield. The generation of PHA from lignocellulose is still in its infancy, hence more screening of lignocellulosic materials and improvements in downstream processing and substrate pre-treatment are needed in the future to further advance the biopolymer sector.

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Section: Carbon Sources or Feedstocks For Pha Productionmentioning

confidence: 99%

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Vigneswari

Noor

Amelia

et al. 2021

Life

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“…However, the PHB production of JH02-pPeftu-(porB)phaC using wheat straw hydrolysate (7.8 g/L and 26.4 wt%) was far lower than that using pure sugars (15.5 g/L and 32.0% w/w). We speculated that the cell surface When different strains were used on PHB production from lignocellulose feedstock, only low cell mass below 10.0 g/L and PHB around 4.0 g/L were obtained due to the inhibitors suppression (Table 3; Gowda & Shivakumar, 2014;Kourilova et al, 2021;Prabu & Murugesan, 2010;Silva et al, 2004;Yu & Stahl, 2008). Although the detoxification led to increased cell growth (below 20.0 g/L) and PHB generation (below 15.0 g/L; de Souza et al, 2020;Kim et al, 2016;Pan et al, 2012;Prabu & Murugesan, 2010;Sawant et al, 2015), these detoxification approaches (evaporation, membrane filtration, and activated carbon) are highly costly with heavy wastes generation and sugar loss, thus not feasible for any practical applications (Jönsson & Martín, 2016).…”

Section: Phb Fermentation In Synthetic Medium and Wheat Straw Hydroly...mentioning

confidence: 99%

“…(Sawant et al, 2015). However, the cell viability of these microbes was generally poor in lignocellulose hydrolysate and the consequent PHB fermentation performance was far below those using glucose as feedstock (Gowda & Shivakumar, 2014; Kourilova et al, 2021; Silva et al, 2004; Yu & Stahl, 2008).…”

Section: Introductionmentioning

confidence: 99%

Engineering Corynebacterium glutamicum for synthesis of poly(3‐hydroxybutyrate) from lignocellulose biomass

Jin

Huang

et al. 2022

Biotech & Bioengineering

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Lignocellulose is the only feasible carbohydrates feedstock for commercial scale and carbon neutral production of poly(3‐hydroxybutyrate) (PHB) biopolymer by its great abundance and availability. Microbial cell factories for fermentative PHB synthesis are highly restricted by the growth suppression of inhibitors from lignocellulose pretreatment. This study targeted a potential PHB‐producing cell factory Corynebacterium glutamicum owing to its strong inhibitors tolerance. A systematic metabolic engineering was conducted starting with the stable PHB synthesis pathway construction from glucose and xylose, followed by the enhancement of PHB synthesis on PHA synthase activity and stability, cell morphology modification, and growth factors regulation. The relocation of the PHA synthase on the cell membrane guided by secrete signal peptides and cell membrane display motifs increased the PHB content by 2.4 folds. Excessive nitrogen preferentially promoted the PHB synthesis capacity and resulted in the PHB content increased by 13.3 folds. Modification of the genes responsible for cell division changed the cell morphology but the cell size was not enlarged to a PHB accumulation favorable environment. The metabolic engineering of C. glutamicum resulted in a high fermentative production of PHB using wheat straw as feedstock. This study provided an important microbial cell factory choice for PHB production using lignocellulose feedstock.

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“…The combination of optimized and engineered marine production strains obtained by "synthetic biology", low or no sterility precautions, and inexpensive cultivation media is being called the "Next Generation Industrial Biotechnology" (NGIB), and holds the potential of being a viable route to producing PHA economically (47). Other auspicious halophilic PHA production strains that fit this NGIB concept are already waiting in the wings, such as the eubacterium Halomonas halophila, a strain with expedient PHA productivity from abundantly available inexpensive raw materials (48,49).…”

Section: Biopolymersmentioning

confidence: 99%

Polyhydroxyalkanoate (PHA) Biopolyesters - Emerging and Major Products of Industrial Biotechnology

Mukherjee¹,

Koller

2022

The EuroBiotech Journal

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Background: Industrial Biotechnology (“White Biotechnology”) is the large-scale production of materials and chemicals using renewable raw materials along with biocatalysts like enzymes derived from microorganisms or by using microorganisms themselves (“whole cell biocatalysis”). While the production of ethanol has existed for several millennia and can be considered a product of Industrial Biotechnology, the application of complex and engineered biocatalysts to produce industrial scale products with acceptable economics is only a few decades old. Bioethanol as fuel, lactic acid as food and PolyHydroxyAlkanoates (PHA) as a processible material are some examples of products derived from Industrial Biotechnology. Purpose and Scope: Industrial Biotechnology is the sector of biotechnology that holds the most promise in reducing our dependence on fossil fuels and mitigating environmental degradation caused by pollution, since all products that are made today from fossil carbon feedstocks could be manufactured using Industrial Biotechnology – renewable carbon feedstocks and biocatalysts. To match the economics of fossil-based bulk products, Industrial Biotechnology-based processes must be sufficiently robust. This aspect continues to evolve with increased technological capabilities to engineer biocatalysts (including microorganisms) and the decreasing relative price difference between renewable and fossil carbon feedstocks. While there have been major successes in manufacturing products from Industrial Biotechnology, challenges exist, although its promise is real. Here, PHA biopolymers are a class of product that is fulfilling this promise. Summary and Conclusion: The authors illustrate the benefits and challenges of Industrial Biotechnology, the circularity and sustainability of such processes, its role in reducing supply chain issues, and alleviating societal problems like poverty and hunger. With increasing awareness among the general public and policy makers of the dangers posed by climate change, pollution and persistent societal issues, Industrial Biotechnology holds the promise of solving these major problems and is poised for a transformative upswing in the manufacture of bulk chemicals and materials from renewable feedstocks and biocatalysts.

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Evaluation of mesophilic Burkholderia sacchari, thermophilic Schlegelella thermodepolymerans and halophilic Halomonas halophila for polyhydroxyalkanoates production on model media mimicking lignocellulose hydrolysates

Cited by 29 publications

References 28 publications

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Engineering Corynebacterium glutamicum for synthesis of poly(3‐hydroxybutyrate) from lignocellulose biomass

Polyhydroxyalkanoate (PHA) Biopolyesters - Emerging and Major Products of Industrial Biotechnology

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