Evaluation of micro- versus nano-electrospray ionization for ambient pressure ion mobility spectrometry

Kanu, Abu B.; Kumar, Bharath S.; Hill, Herbert H.

doi:10.1007/s12127-011-0075-8

Cited by 16 publications

(16 citation statements)

References 49 publications

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“…This is again similar to reports from the ESI-IMS literature for the detection of TNT. 4 , 60 , 61 A calibration curve for TNT ( Supporting Information Figure S8 ) exhibited high linearity ( R 2 = 0.9994) across the dynamic test range of 1–100 ng. A limit of detection (LOD) of 4.02 ng and a relative standard deviation (RSD) of 4.6% across the five data points is evidence of the linearity and repeatability of the Flex-DT-IMS design.…”

Section: Resultsmentioning

confidence: 99%

Flexible Drift Tube for High Resolution Ion Mobility Spectrometry (Flex-DT-IMS)

et al. 2020

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This paper describes, in detail, the development of a novel, low-cost, and flexible drift tube (DT) along with an associated ion mobility spectrometer system. The DT is constructed from a flexible printed circuit board (PCB), with a bespoke “dog-leg” track design, that can be rolled up for ease of assembly. This approach incorporates a shielding layer, as part of the flexible PCB design, and represents the minimum dimensional footprint conceivable for a DT. The low thermal mass of the polyimide substrate and overlapping electrodes, as afforded by the dog-leg design, allow for efficient heat management and high field linearity within the tube–achieved from a single PCB. This is further enhanced by a novel double-glazing configuration which provides a simple and effective means for gas management, minimizing thermal variation within the assembly. Herein, we provide a full experimental characterization of the flexible DT ion mobility spectrometer (Flex-DT-IMS) with corresponding electrodynamic (Simion 8.1) and fluid dynamic (SolidWorks) simulations. The Flex-DT-IMS is shown to have a resolution >80 and a detection limit of low nanograms for the analysis of common explosives (RDX, PETN, HMX, and TNT).

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Section: Resultsmentioning

confidence: 99%

Flexible Drift Tube for High Resolution Ion Mobility Spectrometry (Flex-DT-IMS)

et al. 2020

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“…show that a chromatographic separation is necessary for balancing fluctuating thermo desorption processes and therefore low reproducibilities [15]. An interface for the desorption of SPME fibers prior to DMS detection was developed by Rainsberg and Harrington [28].…”

Section: Introductionmentioning

confidence: 99%

Determination of volatile organic compounds by solid-phase microextraction—gas chromatography-differential mobility spectrometry

Telgheder

Malinowski

Jochmann

2009

Int. J. Ion Mobil. Spec.

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Up to now the analysis of aqueous samples by ion mobility spectrometry is a challenge due to the lack of suitable sample introduction systems. But just the introduction of asymmetric field strengths leading to a higher selectivity and sensitivity, this technique is of growing interest for on-site analysis of contaminated water. In this work, first results for the preparation of a portable GC-DMS system are presented. A differential mobility spectrometer (DMS) with a 63 Ni ionization source has been used as a detector for the gaschromatographic separation of benzene, toluene, ethylbenzene m-, o-, and p-xylene (BTEX) in water. Separation from the matrix and pre-concentration of the analytes was achieved by solid-phase microextraction (SPME). The influence of the different chromatographic parameters, extraction conditions and differential mobility spectrometry settings on the sensitivity and signal shape were investigated. Results of the optimization of the analytical method as well as analytical validation parameters such as method detection limit, limit of quantification and the repeatability are discussed. Additionally, the applicability of the method was demonstrated by the analysis of a real surface water sample.

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“…Furthermore, direct infusion nESI reduces the volume of solvent used by 1000 fold compared to conventional LC-MS [48]. Self-fed systems load sample directly into an emitter to which a voltage is applied initiating a nanospray via capillary action and electrostatic force [33,49,50]. These systems were initially utilised in proteomics, however, they were known to offer poor reproducibility [51][52][53].…”

Section: Self-fed/ Direct Infusion Nanoesimentioning

confidence: 99%

A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage

Chetwynd

David

2018

Talanta

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Liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) platforms are widely used to perform high throughput untargeted profiling of biological samples for metabolomics-based approaches. However, these LC-ESI platforms usually favour the detection of metabolites present at relatively high concentrations because of analytical limitations such as ion suppression, thus reducing overall sensitivity. To counter this issue of sensitivity, the latest in terms of analytical platforms can be adopted to enable a greater portion of the metabolome to be analysed in a single analytical run. Here, nanoflow liquid chromatography-nanoelectrospray ionisation (nLC-nESI), which has previously been utilised successfully in proteomics, is explored for use in metabolomic and exposomic research. As a discovery based field, the markedly increased sensitivity of these nLC-nESI platforms offer the potential to uncover the roles played by low abundant signalling metabolites (e.g. steroids, eicosanoids) in health and disease studies, and would also enable an improvement in the detection of xenobiotics present at trace levels in biological matrices to better characterise the chemical exposome. This review aims to give an insight into the advantages associated with nLC-nESI for metabolomics-based approaches. Initially we detail the source of improved sensitivity prior to reviewing the available approaches to achieving nanoflow rates and nanospray ionisation for metabolomics. The robustness of nLC-nESI platforms was then assessed using the literature available from a metabolomic viewpoint. We also discuss the challenging point of sample preparation which needs to be addressed to fully enjoy the benefits of these nLC-nESI platforms. Finally, we assess metabolomic analysis utilising nano scale platforms and look ahead to the future of metabolomics using these new highly sensitive platforms.

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Evaluation of micro- versus nano-electrospray ionization for ambient pressure ion mobility spectrometry

Cited by 16 publications

References 49 publications

Flexible Drift Tube for High Resolution Ion Mobility Spectrometry (Flex-DT-IMS)

Flexible Drift Tube for High Resolution Ion Mobility Spectrometry (Flex-DT-IMS)

Determination of volatile organic compounds by solid-phase microextraction—gas chromatography-differential mobility spectrometry

A review of nanoscale LC-ESI for metabolomics and its potential to enhance the metabolome coverage

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