Evaluation of microalgal and formulated diets for the culture of the New Zealand pipi clam Paphiesaustralis

Mamat, Nawwar Zawani

doi:10.1007/s40071-014-0057-7

Cited by 24 publications

(9 citation statements)

References 34 publications

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“…calcitrans and I . galbana are considered as good feed in many bivalve mollusks, including Paphies australis [ 27 ], S . constricta [ 28 ] and T .…”

Section: Discussionmentioning

confidence: 99%

qPCR analysis of bivalve larvae feeding preferences when grazing on mixed microalgal diets

et al. 2017

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Characterization of the feeding preferences of bivalve larvae would help improving the bivalve aquaculture and hatchery by providing appropriate microalgal diets. However, inaccurate and laborious identification and counting of microalgal species have challenged the selective feeding of bivalves. In the present study, we developed a highly specific and sensitive assay using quantitative polymerase chain reaction (qPCR) to assess the selective feeding of bivalve larvae based on species-specific primers targeting to microalgal 18S rDNA sequences. The assay exhibited good specificity. The detection limits of the qPCR assay were 769, 71, 781 and 21 18S rDNA copies for Chaetoceros calcitrans, Isochrysis galbana, Platymonas helgolandica and Nannochloropsis oculata, respectively. Using such assay, we found that C. calcitrans and I. galbana were preferentially ingested, whereas N. oculata was preferentially rejected in biodeposits of four bivalve species, Tegillarca gransa, Cyclina sinensis, Scapharca subcrenata and Sinonovacula constricta. Furthermore, our growth experiments revealed that C. calcitrans and I. galbana could significantly promote the shell growth, whereas feeding of N. oculata resulted in poorer growth of four bivalve species. These data indicated that qPCR might be useful in screening of efficient and reliable microalgal species for each bivalve species, leading to improved bivalve aquaculture and hatchery.

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“…calcitrans and I . galbana are considered as good feed in many bivalve mollusks, including Paphies australis [ 27 ], S . constricta [ 28 ] and T .…”

Section: Discussionmentioning

confidence: 99%

qPCR analysis of bivalve larvae feeding preferences when grazing on mixed microalgal diets

et al. 2017

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“…Live microalgae and concentrates were standardized based on dry weight (Mamat & Alfaro 2014). Triplicate samples (100 mL) were filtered using preweighed 1.2-μm GF filters, washed with 50 mL of 0.5 M ammonium formate to remove salt residue, and dried in an oven at 70°C for 18 h (Epifanio 1979).…”

Section: Microalgae Weight Standardizationmentioning

confidence: 99%

Feasibility of Microalgal Concentrates for Production of Post Set Northern Quahogs, Mercenaria mercenaria and Sunray Venus Clams, Macrocallista nimbosa

Perri,

Hassan,

Laramore

2023

Journal of Shellfish Research

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“…Size variations of algae were standardized based on the dry weight and cell density (Abbas et al, 2018;Mamat & Alfaro, 2014). Triplicate 100-ml microalgae samples were filtered through preweighed 1.2μm GF filters, washed with 50 ml 0.5 M ammonium formate to remove salt residue and dried in the oven at 70℃ for 18 hr.…”

Section: Weight Standardization Of Microalgaementioning

confidence: 99%

Assessment of microalgae concentrate as diet for hard clam, Mercenaria mercenaria , larvae

Hassan

Parks²,

Laramore

2021

Aquacult Nutr

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The on-site culture of live microalgae represents a major challenge for bivalve hatchery operations due to high cost and labour demands. This study evaluated the efficacy of commercially available microalgae concentrates as a partial or complete replacement diet for live microalgae for culture of the hard clam, Mercenaria mercenaria, larvae.Larvae were fed from D-larval stage to post-set, 2-14 days post fertilization (DPF).Larvae fed the complete replacement diets were significantly smaller than those fed partially replaced or live microalgae diets. Foot development was significantly delayed in larvae fed complete replacement diets compared to partially replaced or live microalgae diets. Although early survival (at 5 DPF) was similar among the treatments, final survival (at 14 DPF) varied significantly among treatments. The average final survival was 69%-77% for larvae fed live microalgae, 60%-61% for larvae fed partially replaced diets and 14%-16% for larvae fed complete replacement diets. These results suggest that a live microalgae diet could be partially replaced by a concentrate to feed hard clam larvae. However, complete replacement diet may be used only for a short duration (up to 3 days) without affecting survival.

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Evaluation of microalgal and formulated diets for the culture of the New Zealand pipi clam Paphiesaustralis

Cited by 24 publications

References 34 publications

qPCR analysis of bivalve larvae feeding preferences when grazing on mixed microalgal diets

qPCR analysis of bivalve larvae feeding preferences when grazing on mixed microalgal diets

Feasibility of Microalgal Concentrates for Production of Post Set Northern Quahogs, Mercenaria mercenaria and Sunray Venus Clams, Macrocallista nimbosa

Assessment of microalgae concentrate as diet for hard clam, Mercenaria mercenaria , larvae

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