Evaluation of microalgal clones for mass culture in a subtropical greenhouse bivalve hatchery: growth rates and biochemical composition at 30 °C

Nelson, James R.; Guarda, Sonia; Cowell, Lauren E.; Heffernan, Peter B.

doi:10.1016/0044-8486(92)90267-o

Cited by 14 publications

(5 citation statements)

References 42 publications

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“…This result is somewhat higher than the findings of Dunstan et al (1993), which fall in the range $1.70- 2.99\,{\rm pgTFA}\,{\rm cell}^{- 1} $ , and even higher than the range reported for the same species elsewhere: 1.95–2.36 (Brown et al, 1993), 1.98–2.56 (Tzovenis et al, 2003), 1.2 (Volkman et al, 1989), and 1.00–1.37 (Ferreira et al, 2008). The average carbon quota for the WT strain was found to be $15.0\pm 2.2\,{\rm pgC}\,{\rm cell}^{- {\rm 1}} $ and was therefore higher than values previously reported for T‐iso: 5.1–5.8 (Thompson et al, 1993) in turbidostat, 8.6–9.3 (Nelson et al, 1992), and 10.6 (Troedsson et al, 2005). Nevertheless, the high carbon quota reported here may be driven by the N starvation and the concomitant lipid accumulation experienced by the cells during the stationary phase.…”

Section: Resultsmentioning

confidence: 59%

Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T‐Iso) by a mutation‐selection procedure

Bougaran

Rouxel

Dubois

et al. 2012

Biotech & Bioengineering

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Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). Following a first round of mutation-selection, the total fatty acid content had not increased significantly, being 262 ± 21 mgTFA (gC)-1 for the wild type (WT) and 269 ± 49 mgTFA (gC)-1 for the selected population (S1M1). Conversely, fatty acid distribution among the lipid classes was affected by the process, resulting in a 20% increase for the fatty acids in the neutral lipids and a 40% decrease in the phospholipids. After a second mutation-selection step (S2M2), the total fatty acid content reached 409 ± 64 mgTFA (gC)-1 with a fatty acid distribution similar to the S1M1 population. Growth rate remained unaffected by the process, resulting in a 80% increase for neutral lipid productivity.

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Section: Resultsmentioning

confidence: 59%

Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T‐Iso) by a mutation‐selection procedure

Bougaran

Rouxel

Dubois

et al. 2012

Biotech & Bioengineering

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“…galbana only because N . salina can be more easily and less expensively cultured than I. galbana (Morizane 1991; Nelson et al . 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Gireesh and Gopinathan (2004) evaluated the effects of salinity and pH on the growth, survival and metamorphosis of P. malabarica larvae, and found that in clam larvae and juveniles fed with flagellate, I. galbana had the best growth, survival and metamorphosis for salinity 33–35 and pH 7.8–7.9. Nannochloropsis salina is another algal species with higher protein content and of smaller size (2 μm) than I. galbana , which can be easily cultured and is usually less expensive than I. galbana (Morizane 1991; Nelson, Guarada, Cowell & Heffernan 1992). However, no information is available on the effects of N. salina as a single food source or as a partial replacement of I. galbana on the growth, survival and metamorphosis of short neck clam larvae.…”

Section: Introductionmentioning

confidence: 99%

THIS ARTICLE HAS BEEN RETRACTED: Effects of diet, stocking density and environmental factors on growth, survival and metamorphosis of clam, Paphia malabarica (Chemnitz) larvae

Raghavan

Gopinathan

2008

Aquaculture Research

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R e t r a c t e dEffects of diet, stocking density and environmental factors on growth, survival and metamorphosis of clam, Abstract A series of experiments were conducted to evaluate the e¡ects of diet, stocking density and environmental factors on the growth, survival and metamorphosis of short neck clam Paphia malabarica larvae. These experiments examined the following factors: diet [Isochrysis galbana, Nannochloropsis salina and a mixture of I. galbana and N. salina (1:1 w/w)], stocking density (1, 3, 5 and 7 larvae mL À1 ), light intensity (unshaded, partially shaded and fully shaded) and water ¢ltration (un¢ltered and sand ¢ltered). Results indicated that N. salina could replace 50% of I. galbana as a food source for the clam larvae with an increase in growth, survival (47.2%), metamorphosis (33.5%) and early settlement. Larval growth decreased signi¢cantly with increasing stocking density. A density of 1^3 larvae mL À1 appeared to be optimal for normal growth of clam larvae. Neither diet nor stocking density used in the study had a signi¢cant e¡ect on larval survival. Under partially shaded (light intensity 51000^5000 lx) and fully shaded (light intensity o1000 lx) conditions, larval growth was signi¢cantly faster than under direct sunlight (unshaded). Larvae grew signi¢cantly faster in the un¢ltered water than in the ¢ltered water.

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“…(Okauchi et al 1997), Tetraselmis tetrathele (Okauchi and Hirano 1986), and T. chuii (Tobias-Quinitio and Villegas 1982) have high nutritional value and potential growth. Chaetoceros muelleri has a high growth rate (Trujillo-Valle and Voltolina 1994) and it can grow in outdoor conditions with a wide range of temperature and illumination (Nelson et al 1992). Isochrysis galbana is a free living marine unicellular phytoflagellate of the order Chrysomonadales.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of growth, survival and body composition of larval white shrimp (Litopenaeus vannamei) fed the combination of three types of algae

2015

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The larvae of the Pacific white shrimp Litopenaeus vannamei are fed, from the stage of protozoea I until to protozoea III (PZ III), with microalgae. The survival rate, duration of the terms of Z I -Z III and total length of protozoea larvae (PZ) of L. vannamei were survived. The shrimp larvae fed with the six treatments of microalgae (Chaetoceros muelleri, Isochrysis galbana, Tetraselmis tetrathele, C. muelleri : I. galbana, C. muelleri: T. tetrathele and I. galbana: T. tetratheie). The biochemical composition (protein, carbohydrate, lipid and energy) of the algae and the larvae fed those algae were also measured. The largest sizes were recorded for larvae fed with the mixture C. muelleri and I. galbana (4.35 mm) and smaller sizes were observed on larvae fed with I. galbana (3.04 mm) (P \ 0.05). The larvae fed with mixture T. tetrathele and C. muelleri (88.42 %) and I. galbana and T. tetrathele (84.50 %) had the highest survival in experimental treatments. The lowest time for development was observed in larvae fed with mixture of T. tetrathele and C. muelleri (92.6 h). The larvae fed with mixture I. galbana and T. tetrathele had the highest protein and carbohydrate levels as compared with other treatments. Also highest lipid level and gross energy were shown in larvae fed with mixture C. muelleri and I. galbana (P \ 0.05). The results showed that L. vannamei larvae fed with C. muelleri and the mixed diets containing this species (C. muelleri ? Isochrysis sp., C. muelleri ? Tetraselmis sp.) had the highest growth and survival among treatments.

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Evaluation of microalgal clones for mass culture in a subtropical greenhouse bivalve hatchery: growth rates and biochemical composition at 30 °C

Cited by 14 publications

References 42 publications

Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T‐Iso) by a mutation‐selection procedure

Enhancement of neutral lipid productivity in the microalga Isochrysis affinis Galbana (T‐Iso) by a mutation‐selection procedure

THIS ARTICLE HAS BEEN RETRACTED: Effects of diet, stocking density and environmental factors on growth, survival and metamorphosis of clam, Paphia malabarica (Chemnitz) larvae

Evaluation of growth, survival and body composition of larval white shrimp (Litopenaeus vannamei) fed the combination of three types of algae

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