Evaluation of microbial diversity of different soil layers at a contaminated diesel site

Maila, Mphekgo P.; Randima, P.; Surridge, Karen; Drønen, Karine; Cloete, T.E.

doi:10.1016/j.ibiod.2004.06.012

Cited by 32 publications

(29 citation statements)

References 23 publications

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“…Maila et al (2005) demonstrated that the type and concentration of pollutants, rather than the geographical origin of the soil, may be more important in determining the functional diversity and species diversity of such bacterial communities. Because individual bacterial monocultures can metabolise a limited range of hydrocarbon substrates (Britton 1984), a mixed bacterial consortium with broad enzymatic capacities may be more efficient for remediation, and the advantages of using mixed cultures instead of pure cultures in biodegradation processes, such as higher tolerance to temperature, pH and salinity, and higher capacity of produce biosurfactants or extracellular surface-active products, have been amply demonstrated (Boopathy 2000).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Moliterni

Jiménez-Tusset

Rayo

et al. 2012

Int. J. Environ. Sci. Technol.

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Three microbial consortia were isolated from three polluted soils located at an oil refinery and acclimated to grow on diesel fuel as the sole carbon source. Batch experiments were then conducted with the three consortia to study the kinetics of diesel biodegradation. The effects of temperature (25, 30 and 35°C) and diesel concentration (0.5, 1 and 3 %) on the biodegradation of diesel were analysed. Several species were identified in the acclimated microbial consortia, and some of them appeared in more than one consortium. Thermal inhibition was observed at 35°C. In the rest of experiments, over 80 % of the substrate was degraded after 40 h of treatment. These results proved the good feasibility of using the polluted sites as sources of mixed consortia for hydrocarbon degradation. However, diesel degradation efficiencies and rates were very similar, suggesting that the acclimation process produced mixed consortia with very similar characteristics; in this context, origin of the soil sample was not a decisive factor. A simple Monod-type kinetic model was used to simulate the biodegradation process, and accurate results were obtained. The l max values were between 0.17 and 0.34 h -1 . The results of this study revealed that the consortia can function at high concentrations of hydrocarbons without any sign of growth inhibition, which is important for the design of bioreactors for wastewater treatment with high concentrations of fuel.

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Section: Introductionmentioning

confidence: 99%

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Moliterni

Jiménez-Tusset

Rayo

et al. 2012

Int. J. Environ. Sci. Technol.

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“…Because PH contaminants can penetrate deeper than the topsoil layer, it is important to understand how contaminants affect microbial communities along the soil-depth and how microbial activity contributes to biodegradation of penetrating PHs. [10] In addition, the intrinsic biodegradation pathway of PHs should be understood first through monitoring of residual PHs and soil bacterial community for predicting its remedial stage because biodegradation is a site-specific process. [11,12] Studies of microbial community dynamics in PHscontaminated soil layers have been conducted: TPH content and biological activities for TPH degradation were higher in topsoil layer microcosms than in 1-m depth soil layer microcosms.…”

Section: Introductionmentioning

confidence: 99%

Bacterial diversity dynamics in a long-term petroleum-contaminated soil

Lee

Lee²,

Cho³

2011

Journal of Environmental Science and Health, Part A

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Bacterial diversity dynamics were investigated in the soil samples in different distances and depths from/at a long-term petroleum-contaminated site. Microbial activity in the soil samples showed ATP values closely correlated with organic matter content (OC) and total petroleum hydrocarbon (TPH). Bacterial community diversity (H) and evenness (J) using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and PCR-T-RFLP (terminal restriction fragment length polymorphism) results showed positive correlation with concentration of TPH or OC, but tmoA (toluene monooxygenase gene)-based bacterial H and J using a PCR-T-RFLP result did not. No significant difference of H and J values in the bacterial and the tmoA communities was observed. The bacterial community structure characterized by PCR-DGGE and PCR-T-RFLP techniques showed similarity according to soil sampling distance rather than soil sampling depth. Canonical correspondence analysis demonstrated that OC including TPH had the most significant effect on the bacterial community diversity at the long-term petroleum-contaminated site.

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“…Este comportamiento, puede reflejar dependencia de algunas poblaciones a la disponibilidad de fuentes de carbono, derivadas de la biotransformación de la mezcla de hidrocarburos, llevada a cabo por poblaciones microbianas más versátiles (bandas comunes). Cambios en la composición de las comunidades microbianas y selección de poblaciones durante la biodegradación se han reportado previamente utilizando análisis comparativo de los perfiles genó-micos e intensidad de bandas a través del tiempo, en presencia de hidrocarburos y en diferentes tipos de tratamiento en suelo (Maila et al 2005, Supaphol et al 2006. Para el suelo IAN en el periodo analizado (28 a 56 días), la riqueza estimada (S Chao2 ) para ambos tratamientos intemperizados (Fig.…”

Section: Dinámica De La Comunidad Microbiana Nativa En El Ensayo De Bunclassified

Dinámica De Poblaciones Bacterianas Y Actividad Deshidrogenasa Durante La Biorremediación De Suelo Recién Contaminado E Intemperizado Con Hidrocarburos

Ortíz-Maya¹,

Escalante-Espinosa²,

Ramírez-Saad

et al. 2017

Rev. Int. Contam. Ambie.

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Palabras clave: actividad enzimática, DGGE, restauración de suelos, secuenciación RESUMEN Se evaluó la actividad deshidrogenasa y la dinámica de poblaciones bacterianas nativas en suelo contaminado con hidrocarburos durante su biorremediación por bioestimulación y atenuación natural. Se realizaron ensayos a escala mesocosmos, en la que se compararon dos condiciones: 1) contaminación reciente (28 días) y 2) intemperización (entre 28 a 56 días), con suelo artificialmente contaminado (30 000 mg de hidrocarburos totales de petróleo /kg suelo). Se estimó la riqueza de las comunidades bacterianas con base en los perfiles genómicos generados mediante electroforesis en gel con gradiente de desnaturalizantes (DGGE, por sus siglas en inglés). Además, se identificaron las poblaciones por secuenciación de las bandas dominantes en los perfiles de DGGE. La evolución de la biodegradación se estimó a través de la actividad deshidrogenasa durante los ensayos. El análisis molecular de los extractos de ADN, reveló una riqueza de genotipos similar en ambos tratamientos recién contaminados y una disminución importante de este parámetro por efecto de intemperismo. Los niveles máximos de actividad de la enzima se presentaron en el suelo recién contaminado-bioestimulado (3146.84 mg de iodo-nitro-formazan (INF)/g) en comparación con el suelo recién contaminado bajo atenuación natural (1160.19 µg INF/g). Para los suelos intemperizados, la actividad deshidrogenasa disminuyó en comparación con los demás tratamientos. Se determinó una comunidad bacteriana estable a lo largo del tiempo en el suelo intemperizado, mientras que la adición de nutrientes favoreció el incremento de la riqueza de genotipos tanto en los suelos recién contaminados como en los intemperizados. Se identificó la presencia de bacterias hidrocarbonoclastas (Burkholderia, Pelobacter) en todos los tiempos de muestreo en ambos tratamientos.Key words: enzymatic activity, DGGE, soil restoration, sequencing ABSTRACT Dehydrogenase activity and composition of the native microbial community were evaluated in hydrocarbon-contaminated soil during its bioremediation by biostimulation Rev. Int.

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Evaluation of microbial diversity of different soil layers at a contaminated diesel site

Cited by 32 publications

References 23 publications

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Kinetics of biodegradation of diesel fuel by enriched microbial consortia from polluted soils

Bacterial diversity dynamics in a long-term petroleum-contaminated soil

Dinámica De Poblaciones Bacterianas Y Actividad Deshidrogenasa Durante La Biorremediación De Suelo Recién Contaminado E Intemperizado Con Hidrocarburos

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