Evaluation of microleakage of three different types of pit and fissure sealants using invasive and non-invasive techniques (An in-vitro study)

Abstract

Backgrounds:The aims of this study were to; Evaluate the amount of in-vitro microleakage of three different types of pit and fissure sealants (Vertise Flow, Kerr), (Helioseal-F, IvoclarVivadent), (GC Fuji TRI-AGE, GC corporation) and the effect of occlusal preparation on the leakage value.Methods: Sixty extracted human premolars randomly divided into 6 groups (n=10/group). Teeth fissures of three non-invasive groups (1, 3, 5) left intact, fissures of other three invasive groups (2, 4, 6) were opened up with ¼ round bur. Teeth fissures in group (1, 2) sealed with self-adhesive Vertise Flow, group (3, 4) Helioseal-F, while group (5, 6) fissures sealed with Glass Ionomer GC Fuji TRIAGE. The teeththermocycled between 5±2°C and 55±2°C for 500 cycles with a dwell time of 30 seconds; All teeth sealed apically and coated within 1.5 mm of the sealant margin with two layers of nail varnish, then immersed in 1% Methylene blue solution. Subsequently, two buccolingual sections were made parallel to the long axis of tooth yielding 3 sections and 4 surfaces per tooth for microleakage analysis. The surfaces were scored 0 to 3 for extent of microleakage using a binocular microscope at 25X magnification. Microleakage was analyzed by using paired t-test and ANOVA. Results:Invasive technique produced significantly less microleakagethan Non-Invasive groups (P<0.05). In all six groups Helioseal-F in Invasive technique showed significantly the least degree of microleakage (P<0.05). Conclusion:Helioseal-F was the best material in terms of reducedmicroleakage. Invasive techniquecompared to non-invasive technique had produced less degree of microleakage.

J Clin Exp Dent

“…The effectiveness of pit-fissure sealants depends on various conventional factors such as microleakage, penetration capacity, adaptation, bond strength, and viscosity of the sealant ( 28 ). The sealing ability of the restorative materials is the most important factor against microleakage ( 3 ). Sealing grooves, pits, and fissures is considered to be an important procedure among the strategies for preventing or reducing the risk of caries at the initial stages ( 18 ).…”

“…The complex, irregular, and unpredicTable shape of these parts of the tooth favors the formation of caries and makes diagnosis difficult, complicated, and sometimes impossible through classical methods of assessment and diagnosis. Diets and dental plaque easily accumulate in these areas and cannot be removed effectively by the patient ( 3 ). Moreover, lack of saliva flow to the fissures and insufficient intake of remineralization agents do not compensate for a high incidence of occlusal caries ( 4 ).…”

“…Secondly, due to their potential to act as a fluoride reservoir making enamel is more resistant to de-mineralization ( 10 ). In the case of difficult isolation, the use of glass ionomer cement is recommended ( 3 ). Some studies reported the use of bonding agents to enhance the bond strength of sealant to the tooth surface, especially in the case of saliva-contaminated surfaces, however, the technique is not widely used probably due to extended application time and increased cost ( 2 , 11 , 12 ).…”

“…The efficacy of sealants strongly depends on their penetration capability into occlusal areas and maintaining an intimate adaptation of the sealant to the tooth surface ( 3 , 18 ). The marginal sealing ability of sealing material is extremely important for the success of sealants, which can be assessed by evaluating microleakage ( 19 ).…”