Evaluation of micronucleus induction in a Chilean population environmentally exposed to arsenic

Martínez, Valeria P.; Creus, A.; Venegas, W.; Arroyo, Amparo; Beck, Jens; Gebel, Thomas; Surrallés, Jordi; Marcos, Ricard

doi:10.1016/j.mrgentox.2004.07.008

Cited by 41 publications

(25 citation statements)

References 35 publications

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“…The results of other buccal cell micronucleus studies performed on populations exposed to As through drinking water are consistent with our findings [Aposhian et al, 1997;Gonsebatt et al, 1997;Tian et al, 2001;Basu et al, 2002Basu et al, , 2004Ghosh et al, 2006]. Likewise, analysis of people exposed to As in drinking water have demonstrated increased frequencies of MN in exfoliated bladder cells [Warner et al, 1994;Moore et al, 1996Moore et al, , 1997Biggs et al, 1997], and higher frequencies of binucleated micronucleated cells (BNMNs) were found in HPLs from a Chilean population environmentally exposed to As [Martinez et al, 2004]. Increased frequencies of CAs [Nordenson et al, 1978;Nordenson and Beckman, 1982;Nilsson et al, 1993;Dulout et al, 1996;Maki-Paakanen et al, 1998] and SCEs [Lerda, 1994;Hsu et al, 1997;Mahata et al, 2003] have been detected in lymphocytes of individuals exposed to As.…”

Section: Discussionsupporting

confidence: 87%

“…In a Finnish population exposed to As via drinking water, the frequency of CAs in HPLs from persons 30 years of age and above was higher than for younger persons [Maki-Paakanen et al, 1998]. Micronucleus frequencies increased with age among 106 As-exposed individuals and 111 controls from Chile [Martinez et al, 2004]. In contrast, Gonsebatt et al [1997] found higher frequencies of CAs and MN among younger than older As-exposed subjects, and some studies have found no significant associations between cytogenetic endpoints and age [Hsu et al, 1997;Palus et al, 2005].…”

Section: Discussionmentioning

confidence: 92%

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Evaluation of micronucleus frequencies and DNA damage in glass workers exposed to arsenic

Vuyyuri¹,

Ishaq²,

Kuppala³

et al. 2006

Environ and Mol Mutagen

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Arsenic (As) is a known human carcinogen; however, very little is known about the health consequences of occupational exposure to As. In the present study, we assessed the genotoxic damage in the blood cells and in the buccal cells of south Indian glass factory workers who are occupationally exposed to As. The As content in the whole blood of 200 workers and 165 controls was evaluated with inductively coupled plasma mass spectrometry. Blood leukocytes from the subjects were monitored for the level of DNA damage using the Comet assay (mean comet tail length); buccal cells were used to determine the frequency of micronuclei (MN). The mean As concentration was significantly higher in the workers (56.76 microg/L) than in the controls (11.74 microg/L) (P < 0.001). The workers also had increased frequencies of MN in the buccal cells and increased levels of DNA damage in leukocytes compared to the controls (P < 0.001). There were significant correlations between the genotoxicity endpoints that were evaluated and blood As concentration, smoking, age, and the duration of working in the factory. Also, a significant correlation was observed between the frequency of MN and comet tail-length for the worker samples. Our findings indicate that chronic occupational exposure to As is genotoxic and that the Comet assay and micronucleus test are useful assays for evaluating genotoxicity in humans occupationally exposed to As.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 92%

Evaluation of micronucleus frequencies and DNA damage in glass workers exposed to arsenic

Vuyyuri¹,

Ishaq²,

Kuppala³

et al. 2006

Environ and Mol Mutagen

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“…In fact, the group with higher micronuclei frequencies was also the one with the higher mean age. This agrees with previous reports where micronuclei frequencies significantly increase with the age of the analyzed subjects [Barale et al, 1998;Fenech, 1998;Martínez et al, 2004]. Supporting these results, regression analysis showed that age was the only variable that appeared to be significantly linked with the increase of micronuclei frequencies in lymphocytes.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of micronucleus frequencies in blood lymphocytes from smelting plant workers exposed to arsenic

Paiva

Martínez

Creus

et al. 2008

Environ and Mol Mutagen

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Arsenic is a world health problem due to the seriousness of its effects, mainly related with the increase of cancer. In Chile, the geological characteristics of the northern region, associated with the development of the copper mining and smelting activities, have contributed to increase the environmental levels of arsenic. This study has been conducted to investigate the relationship between arsenic occupational exposure and genotoxic effects in smelting plant workers. To determine the genotoxic risk related to the increase of cancer among the chronically exposed population, the frequency of micronuclei in blood (BNMN) has been evaluated. The study was carried out in a total of 207 men, divided into three groups: an exposed group of 105 individuals working in a smelting plant, an internal reference group with 52 employees at the same mine but in the administrative area, and an external reference group of 50 workers from another copper mine where no significant levels of arsenic were detected. As expected, differences between arsenic levels in urine have been detected between groups, with the exposed group being the one with a higher level of total arsenic in urine, followed by the internal reference group. Despite that, no significant differences in micronuclei frequencies in lymphocytes were detected among these groups.

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“…The induction of MN after exposure to As 2 O 3 in our study was found to be dose dependent which is in accordance with the earlier published studies [50,51]. Both CAs and MN are genotoxicity markers [52][53][54][55][56][57]. Although their presence does not lead to adverse effects, their high-level indicates that cell has been exposed to the carcinogens.…”

Section: Sharma and Gillsupporting

confidence: 92%

Restorative Effects of Zingiber Officinale on Arsenic-Induced Genotoxic Effects in Chromosomal Structure of Mice

Sharma

Gill

2018

Asian J Pharm Clin Res

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Objective: The present study investigated the antigenotoxic effect of Zingiber officinale (Ginger) on arsenic-induced genotoxic effects in chromosome structure of Swiss albino mice.Methods: Albino mice were divided into four groups: Group I was kept as control, Group II was administrated an oral dose of arsenic trioxide 5 mg/kg bw for 15 days, Group III was given an oral dose of arsenic trioxide 5 mg/kg bw followed by 20 mg/kg bw of ginger orally, and Group IV was administered with 20 mg/kg bw of ginger orally for 15 days and kept as positive control. The autopsies were done from all the groups on 15-day posttreatment, and bone marrow was removed for chromosomal study and for calculating the frequency of micronuclei (MN). Results:It is concluded from this study that long-term arsenic exposure causes chromosomal anomalies, for example, breaks, gaps, rings, stretching, and stickiness which leads to the fragmentation of chromosomes and increases the frequency of MN. Conclusion:The administration of ginger showed modulatory effects against arsenic-induced genotoxicity.

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Evaluation of micronucleus induction in a Chilean population environmentally exposed to arsenic

Cited by 41 publications

References 35 publications

Evaluation of micronucleus frequencies and DNA damage in glass workers exposed to arsenic

Evaluation of micronucleus frequencies and DNA damage in glass workers exposed to arsenic

Evaluation of micronucleus frequencies in blood lymphocytes from smelting plant workers exposed to arsenic

Restorative Effects of Zingiber Officinale on Arsenic-Induced Genotoxic Effects in Chromosomal Structure of Mice

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