Evaluation of microRNA-223 and microRNA-125a expression association with STAT3 and Bcl2 genes in blood leukocytes of CLL patients: a case–control study

Davari, Nader; Ahmadpour, Fatemeh; Kiani, Ali Asghar; Azadpour, Mojgan; Asadi, Zari Tahannejad

doi:10.1186/s13104-020-05428-0

Cited by 8 publications

(9 citation statements)

References 27 publications

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“…:10.31557/APJCP.2022.23.8.2735 miRNA 223/125a, COBLL1, andROR-1 Expression in B-chronic Lymphocytic Leukemia In our study, we reported that miRNA 223/125a expression was inversely correlated to WBC count and platelet count while it was directly correlated with hemoglobin (Hb) concentration. These findings agreed with Davari et al, (2021). They stated that these miRNAs may have regulatory effects by controlling WBC production based on the inverse correlation between WBC count and Hb concentration.…”

Section: Discussionsupporting

confidence: 84%

“…miRNA 223 and miRNA 125a as tumor suppressor genes target multiple transcription factors like STAT3 (Chen et al, 2012;Fan et al, 2015). Davari et al (2021) found that miRNA-125a and miRNA-223 expression decreased in the patients with CLL compared to the control group. In addition, upregulation of their target genes, BCL-2 and STAT3, was observed in CLL compared to the control subjects.…”

Section: Research Articlementioning

confidence: 97%

“…Dysregulation of the expression of some microRNAs leads to carcinogenesis by influencing cell growth by interfering with cell cycle regulators (Sethi et al, 2014;Nazarian et al, 2019). In CLL, dysregulation of some miRNAs disrupts apoptosis of malignant cells enhancing cell proliferation and disease progression and decreasing patient survival (Davari et al, 2021).…”

Section: Research Articlementioning

confidence: 99%

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Evaluation of miRNA 223/125a and COBLL1 Expressions and ROR-1 Levels as Reliable Markers in B- chronic Lymphocytic Leukemia

Hussein

Abdelazem

Abdelmoneem

et al. 2022

Asian Pac J Cancer Prev

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Background: miRNA 223 /125a and Cordon-bleu Protein Like 1 (COBLL1) are novel biomarkers that can predict prognosis and guide treatment decisions in patients with chronic lymphocytic leukemia (CLL). Also, there is a growing interest in CLL monitoring based on flow cytometry of receptor tyrosine kinase-like orphan receptor-1 (ROR-1).Objective: This study aimed to evaluate the relationship between miRNA 223 /125a and COBLL1 expressions and ROR-1 expression in patients with CLL. Also, the study evaluated the relationship between the expression of these biomarkers with tumor staging and cancer progression. Methods: Our study included 40 patients newly diagnosed with B-CLL. In peripheral blood (PB), miRNA 223/125a and COBLL1 expressions were detected by real-time polymerase chain reaction (real-time PCR) and ROR-1 percentage was detected by flow cytometry before and after treatment. Results: High level of COBLL1 expression was statistically significantly associated with high ROR-1 percentage expression (P= 0.03). However, a high level of miRNA 223/125a expression was statistically significantly associated with low ROR-1 percentage expression (P=0.002). The sensitivity and specificity of ROR-1 as a predictor of high WBCs count after treatment were 96.6 and 81.1%, respectively. There was a statistically significant reduction of ROR-1 percentage after treatment compared to before treatment (P <0.001). Conclusion: ROR-1 percentage expression can be considered a possible prognostic predictor in CLL along with miRNA 223/125a and COBLL1 expressions. This can be explained by the significant correlation between ROR-1 and the studied molecular biomarkers; miRNA 223/125a and COBLL1. In addition, there was a significantly higher ROR-1 percentage in patients with higher WBC counts. Moreover, there was a significant reduction in ROR-1 percentage after treatment.

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Section: Discussionsupporting

confidence: 84%

Section: Research Articlementioning

confidence: 97%

Section: Research Articlementioning

confidence: 99%

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Evaluation of miRNA 223/125a and COBLL1 Expressions and ROR-1 Levels as Reliable Markers in B- chronic Lymphocytic Leukemia

Hussein

Abdelazem

Abdelmoneem

et al. 2022

Asian Pac J Cancer Prev

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“…Its usefulness is illustrated with the GAPDH phylogeny (Data set 5). Briefly, two different trees were built after enriching the same starting set of sequences from high-quality proteomes [ 19 ], one using all 570 samples from plastid-bearing organisms and the other using 172 transcriptomes resulting from our pipeline. The comparison of these two trees shows that the enrichment performed with the data set described here is less contaminated, less redundant and more balanced from a taxonomic point of view, thereby allowing more robust evolutionary interpretations.…”

Section: Data Descriptionmentioning

confidence: 99%

Decontamination, pooling and dereplication of the 678 samples of the Marine Microbial Eukaryote Transcriptome Sequencing Project

et al. 2021

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Objectives Complex algae are photosynthetic organisms resulting from eukaryote-to-eukaryote endosymbiotic-like interactions. Yet the specific lineages and mechanisms are still under debate. That is why large scale phylogenomic studies are needed. Whereas available proteomes provide a limited diversity of complex algae, MMETSP (Marine Microbial Eukaryote Transcriptome Sequencing Project) transcriptomes represent a valuable resource for phylogenomic analyses, owing to their broad and rich taxonomic sampling, especially of photosynthetic species. Unfortunately, this sampling is unbalanced and sometimes highly redundant. Moreover, we observed contaminated sequences in some samples. In such a context, tree inference and readability are impaired. Consequently, the aim of the data processing reported here is to release a unique set of clean and non-redundant transcriptomes produced through an original protocol featuring decontamination, pooling and dereplication steps. Data description We submitted 678 MMETSP re-assembly samples to our parallel consolidation pipeline. Hence, we combined 423 samples into 110 consolidated transcriptomes, after the systematic removal of the most contaminated samples (186). This approach resulted in a total of 224 high-quality transcriptomes, easy to use and suitable to compute less contaminated, less redundant and more balanced phylogenies.

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“…The importance of miRNAs in CLL progno-According to the results of this study, hsa-miR-29a, hsa-miR-150-5p, and hsa-miR-155-5p were moderately predictive biomarkers for CLL risk, while no correlation between hsa-miR-16-5p and hsa-miR-223-3p levels and the risk of CLL was detected[131]. Recently, it was discovered that CLL patients have decreased levels of miR-223 and miR-125a, as well as greater levels of their targets BCL-2 and STAT3[132].…”

mentioning

confidence: 99%

MicroRNAs: Potential prognostic and theranostic biomarkers in chronic lymphocytic leukemia

Ali,

Mahla,

Reza

et al. 2024

eJHaem

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Small noncoding ribonucleic acids called microRNAs coordinate numerous critical physiological and biological processes such as cell division, proliferation, and death. These regulatory molecules interfere with the function of many genes by binding the 3'‐UTR region of target mRNAs to inhibit their translation or even degrade them. Given that a large proportion of miRNAs behave as either tumor suppressors or oncogenes, any genetic or epigenetic aberration changeing their structure and/or function could initiate tumor formation and development. An example of such cancers is chronic lymphocytic leukemia (CLL), the most prevalent adult leukemia in Western nations, which is caused by unregulated growth and buildup of defective cells in the peripheral blood and lymphoid organs. Genetic alterations at cellular and molecular levels play an important role in the occurrence and development of CLL. In this vein, it was noted that the development of this disease is noticeably affected by changes in the expression and function of miRNAs. Many studies on miRNAs have shown that these molecules are pivotal in the prognosis of different cancers, including CLL, and their epigenetic alterations (e.g., methylation) can predict disease progression and response to treatment. Furthermore, miRNAs are involved in the development of drug resistance in CLL, and targeting these molecules can be considered a new therapeutic approach for the treatment of this disease. MiRNA screening can offer important information on the etiology and development of CLL. Considering the importance of miRNAs in gene expression regulation, their application in the diagnosis, prognosis, and treatment of CLL is reviewed in this paper.

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Evaluation of microRNA-223 and microRNA-125a expression association with STAT3 and Bcl2 genes in blood leukocytes of CLL patients: a case–control study

Cited by 8 publications

References 27 publications

Evaluation of miRNA 223/125a and COBLL1 Expressions and ROR-1 Levels as Reliable Markers in B- chronic Lymphocytic Leukemia

Evaluation of miRNA 223/125a and COBLL1 Expressions and ROR-1 Levels as Reliable Markers in B- chronic Lymphocytic Leukemia

Decontamination, pooling and dereplication of the 678 samples of the Marine Microbial Eukaryote Transcriptome Sequencing Project

MicroRNAs: Potential prognostic and theranostic biomarkers in chronic lymphocytic leukemia

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